摘要： 以20个不同萝卜品种为试材，采用高效液相色谱法（HPLC）研究了幼苗期硫代葡萄糖苷的含量与组成。结果表明：不同品种幼苗中总硫苷含量不同，其中，秋白萝卜〉春白萝卜〉樱桃萝卜。进一步对硫苷组成进行分析，发现了9种不同的硫苷，其中，4种硫苷属于脂肪族，4种属于吲哚族，1种属于芳香族硫苷。不同类型品种幼苗中的硫苷组成不尽相同。秋白及4个春白萝卜品种中含有上述9种硫苷；春白萝卜品种二年子和花知中含有8种硫苷，缺少1个吲哚族硫苷-1-甲氧基吲哚-3-甲基硫代葡萄糖苷；而所有樱桃萝卜品种中均缺少1个脂肪族硫苷-4-甲硫基丁基硫代葡萄糖苷。在对不同萝卜品种幼苗中各种硫苷所占总硫苷百分比分析时发现：秋白萝卜品种（除夏季外）及春白萝卜品种的幼苗中均以4-甲硫基-3-丁烯基硫代葡萄糖苷为主，该类型的硫苷占总量的45％-77％，其次是吲哚-3-甲基硫代葡萄糖苷，夏季品种中上述两种硫苷的含量相当。樱桃萝b幼苗中的硫苷以吲哚一3．甲基硫代葡萄糖苷为主（42％～72％）。在以上几种硫苷中，4-甲硫基-3-丁烯基硫苷是秋白萝卜与春白萝卜中的主要硫苷，在硫代葡萄糖苷酶的作用下，可被转化成异硫氰酸盐。该物质是一种解毒酶的诱发剂，在肝癌细胞系中起解毒作用。上述研究结果为萝卜的品种改良及高硫苷含量萝卜新品种的选育提供了重要信息。

关键词: 萝卜, 幼苗, 硫代葡萄糖苷

Abstract: Glucosinolates composition and contents were evaluated in radish seedlings of 20 genotypes including three groups- autumn-white radish, spring-white radish and cherry radish. The total glucosinolate content varied with the groups. The autumn-white varieties had the highest total glueosinolate content, followed by spring-white varieties and cherry radishes. Nine types of glucosinolates were identified in the radish seedlings. And the aromatic glucosino- lates were reported in radish for the first time. The main glucosinolate in autumn-white radish and spring-white rad- ish was 4-methylthio-3-butenyl-glucosinolates which made up 45% -77% of the total amount. In cherry radish,the indol-3-mehtyl glucosinolate was the predominant glucosinolate which made up 42% -72% of the total amount. 4- methyhhio-3-butenyl-glucosinolates,the main glueosinolate in white radish seedlings,is benefit for human＇s health as a detoxification in the hepatoma cell line.

Key words: Radish, Seedlings, Glucosinolates

中图分类号: 

• S631.1