摘要： 以胡卢巴幼苗为试验材料,研究NaCl胁迫下胡卢巴幼苗叶和根中抗氧化酶SOD、POD、CAT活性及丙二醛(MDA)含量的变化。结果表明:随着NaCl处理浓度的升高,SOD、POD、CAT活性总体呈现先上升后下降的趋势,MDA含量则呈上升趋势。叶片中抗氧化酶活性在NaCl浓度为1.0%时达到峰值,根中抗氧化酶活性在NaCl浓度为0.5%时达到峰值。在0.5%的NaCl浓度处理下,随着处理时间的延长,3种抗氧化酶协同作用,使MDA含量减少并控制在较稳定的阶段。可见,盐胁迫下胡卢幼苗可通过提高抗氧化酶活性,降低膜质过氧化水平,减缓盐胁迫对植株的伤害,从而增强其耐盐性。
关键词:
胡卢巴,
NaCl胁迫,
抗氧化酶,
丙二醛
Abstract: The activities of SOD，POD，CAT and the content of MDA in Trigonella foenum-graecum L． seedlings?were studied under NaCl stress． The results showed that as the NaCl concentration increasing，the activities of SOD, POD，CAT increased at first and then decreased，and the content of MDA showed an increasing trend． Among all?treatments，the activities of SOD，POD，CAT got the peak when NaCl concentration was 1． 0% in the leaves and 0．?5% in the roots． In the treatment of 0． 5% NaCl concentration，three antioxidant enzymes worked together to resist?the injury from NaCl stress． The results indicated that the seedlings of Trigonella foenum-graecum L． could raise the?activities of antioxidant enzymes and decrease the membrane lipid peroxidation under salt stress，which could alleviate the damage of salt stress and improve the salt tolerance．
Key words:
Trigonella foenum-graecum L． ,
NaCl stress,
Antioxidant enzymes,
MDA
中图分类号:
李慧, 王妙媛, 彭立新, 林木强, 阎国荣. NaCl胁迫对胡卢巴幼苗抗氧化酶活性和丙二醛含量的影响[J]. 华北农学报, 2012, 27(2): 185-188. doi: 10.3969/j.issn.1000-7091.2012.02.035.
LI Hui, WANG Miao-yuan, PENG Li-xin, LIN Mu-qiang, YAN Guo-rong. Effects of NaCl Stress on Activities of Antioxidant Enzymes and MDA Content in Trigonella foenum-graecum L． Seedlings[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 185-188. doi: 10.3969/j.issn.1000-7091.2012.02.035.