摘要： 鸭瘟是鸭的一种急性传染和高致死的病毒性疾病。根据GenBank收录中鸭瘟病毒gG基因序列设计并合成1对引物,以鸭瘟病毒河南分离株DNA为模板,PCR扩增出1条约1 600 bp的含gG基因特异性片段,并将其克隆至PMD19-T载体上。经酶切鉴定,得到含gG基因重组质粒,并对重组阳性质粒进行序列测定。结果表明,gG基因长1 380 bp,该基因编码459个氨基酸,预测分子量为50.5 kDa。gG基因的克隆为进一步研究其生物学功能奠定了基础。

关键词: 鸭瘟病毒, gG基因, 克隆, 测序

Abstract: Duck Plague is an acute contagious and highly lethal virus disease of ducks． According to published Duck Plague Virus gGgene sequences，one pair of specific primers was designed and synthesized． The genomic DNA of DPV Henan strain，which was isolated from Henan，was used as template． Full-length gGgene was amplified by polymerase chain reaction(PCR) and cloned into the PMD19-T vector． The gene was sequenced and the open reading frame of 1 380 bp was determined． it encode 459 amino acids and the predicted protein has an molecular mass of 50． 5 kDa. The clone of gGgene provides foundation for study of its biological function．

Key words: Duck Plague Virus(DPV), gGgene, Cloning, Sequencing

中图分类号: 

• S858． 31