摘要： 谷子弯孢病菌(Cochliobolus lunatus)引起的谷子叶斑病是谷子生产上的重要病害之一,常造成严重经济损失.本研究利用候选基因法和RACE技术克隆了谷子弯孢病菌中的1个Ga基因,命名为Clga-1,在GeneBank登陆号为HQ699081.Clga-1基因开放阅读框为l 062 bp,编码353个氨基酸多肽,该开放阅读框被3个大小分别为57,9,59 bp的内含子隔开.聚类分析结果表明,谷子弯孢病菌Clga-1基因与其他植物病原真菌Ga基因的同源性很高,Southern杂交结果表明该基因以单拷贝形式存在.Clga-1基因具有其他病原真菌Ga基因具有的保守结构,如在氮端有豆蔻酰化位点,碳端具有ADP核糖基化位点.谷子弯孢病菌Clga-1基因的克隆,为进一步研究Clga-1基因在该菌致病过程中的功能奠定基础.

关键词: 谷子弯孢病菌, 异三聚体G蛋白, RACE, 序列分析

Abstract: Cochliobolus lunatus is a destructive pathogen of foxtail millet and causes significant yield losses.A G protein alpha protein genes named cfga-J(Genbank accession number：HQ69908 1)was cloned using degenerate PCR and RACE method from Cochliobolus lunatus.The predicated C/as-J gene contains a 1062 bp ORF and it was interrupted by three small introns of 57,49,59 bp.The Ctga-J gene encodes a polypeptide of 353 amino acids and phylogenetic analysis showed CtgoL-1 gene was similarity to the Ga subunit of other fungi.The analysis of Southern bloting indicated that there was a single copy of Clgct-j gene in the genome.Clga-Z gene also contained conservative motifs among the Got protein family,such as sites for myristoylation at the N-terminus,ADP-ribosylation by pertussis toxin at the C-terminus.The cloning of the gene will lay afoundation for further functional analysis of heterotrimericG protein signal transduction pathway in Cochliobolus lunatus.

Key words: Cochliobolus lunatus, Heterotrimeric G protein, RACE, Equence analysis

中图分类号: 

• Q785