外源NO浸种对NaCl胁迫下沙葱种子萌发和生理特性的影响

doi:10.7668/hbnxb.2011.02.043

摘要/Abstract

摘要： 为了探究外源NO浸种对盐胁迫下沙葱种子萌发及抗氧化物酶系活性的影响,以沙葱种子为材料,观察不同浓度和浸种时间的外源NO处理对NaCl胁迫下沙葱种子萌发、子叶、胚根长度及干质量、抗氧化酶系和MDA变化的影响.研究结果表明,经0.40 mmol⁄L SNP浸种3 h能显著(P＜0.05)提高沙葱种子在NaCl胁迫下的萌发率、萌发速率、萌发指数、活力指数、以及子叶和胚根的长度和干质量;该浸种处理能有效地提高NaCI胁迫下种子内SOD、CAT、POD和APX抗氧化酶的活性,从而增强种子萌发抗NaCl胁迫的能力;NaCl胁迫处理促进了沙葱种子细胞的膜脂过氧化,使其MDA含量提高;但0.40 mmol⁄L外源NO处理明显缓解了这种氧化损伤,降低了种子的膜脂过氧化作用.

关键词: 沙葱, 种子萌发, NaCl胁迫, 抗氧化物酶, 外源N0

Abstract: In order to investigate the effects of seed soaking with exogenous NO on seed germination,activities of antioxidant enzymes of AUium mongolicum Regel.under NaCI stress.The experiment used the seed of A.mongolicum as experiment material to explore the effect of different concentrations of NO soaking different times on A.mongolicum seed germination,hypocotyl,radicle length and dry weight,a8 well as the effect of SOD,CAT,POD,APX and content of MDA.In our study,the soaked seeds with 0.40 mmoL/L NO for 3 hours could significantly(P<0.05)enhance the germination percentage,germination rate,germination index,vigor index,length and dry weigh of cotyledon and radicle,meanwhile this treatment could increase the activitvies of antioxidant enzymes：SOD,CAT,POD,APX additionally under NaCI stress,lipid proxidation of the seed of A.mongolicum W88 promoted,therefore the content of MDA was increased,but oxidative damage of seed is relieved under the treatment of 0.40 mmol/L exogenous NO and lipid proxidation was reduced.

Key words: AUium mongolicum Regel, Seed germination, NaCI stress, Antioxidant enzymes, Exogenous NO

中图分类号:

Q949.71

路莹, 王萍, 宋兆伟. 外源NO浸种对NaCl胁迫下沙葱种子萌发和生理特性的影响[J]. 华北农学报, 2011, 26(2): 207-212. doi: 10.7668/hbnxb.2011.02.043.

LU Ying, WANG Ping, SONG Zhao-wei. Effect of Seed Soaking with Exogenous NO on Seed Germination and Physiological Characteristics of Allium mongolicum Regel.under NaCl Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 207-212. doi: 10.7668/hbnxb.2011.02.043.

http://www.hbnxb.net/CN/Y2011/V26/I2/207

参考文献

[1] Bethke P C,Libourel I G L,Jones R L.Nitric oxide reduces seed dormancy in Arabidopsis[J].Experimental Botany,2006,57:517-526.

[2] St(o)hr C,Stremlau S.Formation and possible roles of nitric oxide in plant roots[J].Journal of Experimental Botany,2006,57:463-470.

[3] Simontacchi M,Jasid S,Puntarulo S.Nitric oxide generation during early germination of sorghum seeds[J].Plant Science,2004,167:839-847.

[4] Hu K D,Hu L Y,Li Y H,et al.Protective roles of nitric oxide on germination and antioxidant metabolism in wheat seeds under copper stress[J].Plant Growth Regulation,2007,53:173-183.

[5] Guo Y Q,Tian Z Y,Yan D L,et al.Effects of nitric oxide on salt stress tolerance in Kosteletzkya virginica[J].Life Science Journal,2009,6 (1):67-75.

[6] Li W,Liu X,Khan M A,et al.The effect of plant growth regulators,nitric oxide,nitrate,nitrite and light on the germination of dimorphic seeds of Suaeda salsa under saline conditions[J].Plant Research,2005,118:207-214.

[7] Zheng C F,Jiang D,Liu F,et al.Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity[J].Environmental and Experimental Botany,2009,67:222-227.

[8] 马全林,刘世增,严子柱,等.人工栽培沙葱的光合生理特征[J].西北植物学报,2006,26(1):127-132.

[9] 张瑞霞.野生蔬菜简介[J].内蒙古农业科技,1995(5):34.

[10] 李红,王六英,那顺.内蒙古西泉乡山野菜资源的调查[J].内蒙古农业科技,2003(5):26-28.

[11] 张美丽,高聚林.蒙古韭生物学特性及营养价值初探[J].内蒙古农业科技,1997(5):25-26.

[12] 马强,张汉达.丰富的山野蔬菜[J].内蒙古农业科技,1996(2):38.

[13] 严子柱,刘世增,李爱德,等.野生蔬菜资源-沙葱的开发价值初探[J].甘肃林业科技,2006,31(1):8-11.

[14] 蒋涛,敖长金.沙葱的研究进展[J].现代农业科技,2008,9:10-11,14.

[15] 赵文智,荔克让,何欣东.我国不同地带沙地盐分特征研究[J].地理科学,1995,15(4):315-320.

[16] Ueno K.Effect of desiccation and a change in temperature on germination of immature grains of wheat (Triticum aestivum L.)[J].Euphytica,2002,126:107-113.

[17] Song J,Feng G,Zhang F.Salinity and temperature effects on germination for three salt-resistant euhalophytes,Halostachys caspica,Kaliolium foliatum and Halocnemum strobilaceum[J].Plant and Soil,2006,279:201-207.

[18] Br(a)ndel M.Effect of temperature on dormancy and germination in three species in the Lamiaceae occurring in northern wetlands[J].Wetlands Ecology and Management,2006,14:11-28.

[19] Baker K S,Steadman K J,Plummer J A,et al.Seed dormancy and germination responses of nine Australian fire ephemerals[J].Plant and Soil,2005,277:345-358.

[20] Kepczytiski J,Bihum M.Induction of secondary dormancy in Amaranthas caudatus seeds[J].Plant Growth Regulation,2002,38:135-140.

[21] Timson J.New method of recording germination data[J].Nature,1965,207:216-217.

[22] Khan M A,Ungar I A.The effect of salinity and temperature on germination of polymorphic seeds and growth of Atriplex triangularis Willd[J].American Journal of Botany,1984,71:481-489.

[23] 苗春乐,郝丽珍,王萍,等.NaCI胁迫对沙葱种子生活力及抗氧化酶活性的影响[J].华北农学报,2008,23(4):172-175.

[24] Cakmak I,Marschner H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase ascorbate peroxidase and glutathione reductase in bean leaves[J].Plant Physiology,1992,98:1222-1227.

[25] Schoner S,Krause H.Protective systems against active oxygen species in spinach:response to cold acclimation in excess light[J].Planta,1990,180:383-389.

[26] 郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004:110-113.

[27] 张立军,樊金娟.植物生理学实验教程[M].北京:中国农业大学出版社,2007:103-104.

[28] Kozi A.Ascorbate peroxidase-a hydrogen peroxide scavenging enzyme in plants[J].Physiologia Plantarum,1992,85:235-241.

[29] 郑炳松.现代植物生理生化研究技术[M].北京:气象出版社,2006:90-92.

[30] 王学奎.植物生理生化实验原理与技术[M].北京:高等教育出版社,2006:280-281.

[31] Khan M A,Ungar I A.Influence of salinity and temperature on the germination of Haloxylon recurvum Bunge ex.Boiss[J].Annals of Bothany,1996,78:547-551.

[32] Khan M A,Gul B,Weber D J.Influence of salinity and temperature on the germination of Kochia scoparia[J].Wetlands Ecology and Management,2001,9:483 -489.

[33] 聂惠,于海峰,刘浩明.向日葵对盐胁迫的反应及其抗盐机理的研究进展[J].内蒙古农业科技,2008(6):17-19.

[34] 宋晓艳,安君.盐胁迫对野大豆种子萌发特性的影响[J].内蒙古农业科技,2008,1:41-43.

[35] Egan T P,Ungar I A,Meekins J F.The effect of different salts of sodium and potassium on the germination of Atriplex prostrata (Chenopodiaceae)[J].Plant Nutrition,1997,20:1723-1730.

[36] 黄雅琴.几种作物发芽的耐盐力试验[J].内蒙古农业科技,1979,5:29-32.

[37] 孙方行,孙明高.盐胁迫对臭椿的生长与光合的影响[J].内蒙古农业科技,2009,5:35-36.

[38] Kopyra M,Gw(o)(z)d(z) E A.Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus[J].Physiology and Biochemistry,2003,41:1011-1017.

[39] Gould K S,Lamotte O,Klinguer A,et al.Nitric oxide production in tobacco leaf cells:a generalized stress response?[J].Plant Cell Environment,2003,26:1851 -1862.

[40] Zhao L,Zhan F,Guo J,et al.Nitric oxide functions as a signal in salt resistance in the calluses from two ecotypes of reed[J].Plant Physiology,2004,134:849-857.

[41] Shi Q H,Ding F,Wang X F,et al.Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress[J].Plant Physiology and Biochemistry.2007.45:542-550.

[42] 王宪叶,沈文飚,徐朗莱.外源一氧化氮对渗透胁迫下小麦幼苗叶片膜脂过氧化的缓解作用[J].植物生理与分子生物学学报,2004,30:195-200.

[43] 阮海华,沈文飚,叶茂炳,等.一氧化氮对盐胁迫下小麦叶片氧化损伤的保护效应[J].科学通报,2001,46:1993-1997.

[44] Clark D,Durner J,Navarre D A,et al.Nitric oxide inhibition of tobacco catalase and ascorbate peroxidase[J].Molecular Plant-Microbe Interactions,2000,13:1380 -1384.

[45] Uchida A,Andre T J,Takashi H,et al.Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice[J].Plant Science,2002,163:515 -523.

[46] 汤绍虎,周启贵,孙敏,等.外源NO对渗透胁迫下黄瓜种子萌发、幼苗生长和生理特性的影响[J].中国农业科学,2007,40(2):419-425.

选择文件类型/文献管理软件名称

选择包含的内容