花生AFLP遗传图谱构建及青枯病抗性QTL分析

doi:10.7668/hbnxb.2010.06.015

摘要/Abstract

摘要： 青枯病抗性分子标记能为花生抗青枯病育种提供辅助选择技术,以抗青枯病品种远杂9102与感病品种Chico杂交构建的重组自交系群体(RIL)为材料,用66对具多态性的引物(EcoR I/MseI引物组合35对,MluI/MseI引物组合14对,P_stI/MseI引物组合17对)对其进行扩增,共检测到324个多态性位点。应用JoinMap(3.0软件对这些多态性位点进行遗传连锁分析,构建了一张栽培种花生的AFLP遗传连锁图。该图谱包含98个AFLP标记,涉及20个连锁群,覆盖总距离285 cM,标记间平均图距为2.90 cM。结合RIL群体的青枯病抗性鉴定结果,利用分析软件QTLNetwork(2.0共检测到与青枯病抗性相关的3个QTL(qBWr1、qBWr2和qBWr3)。其中,qBWr1和qBWr2均位于第4连锁群,qBWr3位于第14连锁群。这3个QTL形成2对具有加性×加性上位性互作效应的QTL区段(qBWr1/qBWr3和qBWr2/qBWr3),贡献率分别为12.81%和16.56%,共解释青枯病抗性总变异的21.62%。

关键词: 花生, AFLP, 连锁图, 青枯病抗性, QTL

Abstract: Eighty—two recombinant lines(RILs)derived from the cross between peanut genotypes Yuanza9 1 02 and Chico were employed as genetic linkage mapping population.324 segregating loci were amplifide by 66 AFLP polymorphic primer pairs.Based on the AFLP data.a linkage map of cultivated peanut was constructed using JoinMap3.0 software, which consisted of 98 AFLP markers involvde in 20 linkage groups and covered a total of 285 cM of the genome with an average interval distance of2.90 cM.Combined with the result of bacterial wilt resistance(BW。)evaluated, 3 QTLs(qBW’I, qBW2, qBWr3)for peanut BW。were detected using QTLNetwork2.0 software.qBIVl andqBIV2 were located in group4 while qBW'3 in group 14.These 3 QTLs formed 2 QTL combinations(qBW'I/qBW'3&qBW'2/qBW73)with additive by additive epistasis effects which contributed to qBIV with 12.81%and 16.56%respectively, and explained 21.62%of the total phonotypic variance for BW’.

Key words: Peanut(Arachis hypogaea L.), AFLP, Genetic linkage map, Bacterial wih resistance, QTL

中图分类号:

S565.2

彭文舫, 姜慧芳, 任小平, 吕建伟, 赵新燕, 黄莉. 花生AFLP遗传图谱构建及青枯病抗性QTL分析[J]. 华北农学报, 2010, 25(6): 81-86. doi: 10.7668/hbnxb.2010.06.015.

PENG Wen-fang, JIANG Hui-fang, REN Xiao-ping, LU Jian-wei, ZHAO Xin-yan, HUANG Li. Construction of AFLP Genetic Linkage Map and Detection of QTLs for Bacterial Wilt Resistance in Peanut(Arachis hypogaea L.)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 81-86. doi: 10.7668/hbnxb.2010.06.015.

http://www.hbnxb.net/CN/Y2010/V25/I6/81

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