摘要： 采用紫外诱变、化学诱变(DES诱变)及复合诱变的方法对产纤维素酶枯草芽孢杆菌(Bacillus subtilis)B6的原生质体进行诱变,选育出10个高纤维酶活突变株.摇瓶发酵试验结果表明,所选突变株酶活都显著高于B6,其中,紫外诱变处理的突变株Z12,化学诱变得到的突变株H1以及复合诱变处理的突变株F12的产酶能力相对较强,且产酶能力稳定,酶活值分别为8.3,50.9,91.8 U/mL,分别为对照的176%,178%,19%.试验结果说明,对枯草芽孢杆菌的原生质进行诱变可以提高菌株产纤维素酶的能力,而原生质体的复合诱变可提高诱变效应.
关键词:
枯草芽孢杆菌,
原生质体,
紫外诱变,
化学诱变,
复合诱变,
纤维素酶
Abstract: The Bacillus subtilis B6 isolated from environment was used for protoplast Ultraviolet(UV) mutagenesis,diethyl sulfate(DES) mutagenesis and combination mutagenesis to screen high cellulase activity mutant strains. The results showed that the cellulase production of 10 excellent mutant strains chosen from 342 mutants was significant higher than B6 through fermentation culture in shaking flasks. The cellulase activities of Z12 from UV mutagenesis,H1 from DES mutagenesis and F12 from combination mutagenesis were 448. 3 ,450. 9 and 491. 8 U /mL respectively, which were more than 70% than that of original strain,further more the mutants producing cellulase abilities had no change over 5 generations. The experimental results showed that the producing cellulase ability of Bacillus Subtilis could be increased through protoplast mutagenesis and combination mutagenesis could improve the mutagenic effect.
Key words:
Bacillus subtilis,
Protoplast,
UV mutagenesis,
DES mutagenesis,
Combination mutagenesis,
Cellulase
中图分类号:
谢凤行, 张峰峰, 周可, 赵玉洁, 刘韵娅. 原生质体诱变选育高纤维素酶活性枯草芽孢杆菌的研究[J]. 华北农学报, 2010, 25(5): 211-214. doi: 10.7668/hbnxb.2010.05.043.
XIE Fengxing, ZHANG Fengfeng, ZHOU Ke, ZHAO Yujie, LIU Yunya. The Mutagenesis of Bacillus subtilis Protoplast for Screening Highly Producing Cellulase Strains[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 211-214. doi: 10.7668/hbnxb.2010.05.043.