我国小麦主推品种穗发芽抗性鉴定及相关分子标记的评价

doi:10.7668/hbnxb.2010.04.002

华北农学报 ›› 2010, Vol. 25 ›› Issue (4): 6-11. doi: 10.7668/hbnxb.2010.04.002

所属专题： 小麦；

我国小麦主推品种穗发芽抗性鉴定及相关分子标记的评价

孙果忠^1,2, 游光霞¹, 孙京燕², 张秀英¹, 武淑祯², 苑菲¹, 王海波², 肖世和¹

1. 中国农业科学院作物科学研究所, 北京 100081;
2. 河北省农林科学院遗传生理研究所, 河北石家庄 050051

收稿日期:2010-06-19 出版日期:2010-08-28
通讯作者: 肖世和(1955- )，男，四川广汉人，研究员，博士，主要从事小麦遗传育种研究。王海波(1960- )，男，河北满城人，研究员，博士，主要从事小麦遗传育种研究。
作者简介:孙果忠(1973- ),男,河北青县人,副研究员,博士,主要从事小麦遗传育种研究.
基金资助:
国家重点基础研究发展计划项目(2009CB118306);中国农业科学院委托课题(nycytx-03);河北省财政专项(2009055001)

Identification on Pre-harvest Sprouting Resistance and Evaluation to Related Molecular Markers in Chinese Wheat Cultivars

SUN Guo-zhong^1,2, YOU Guang-xia¹, SUN Jing-yan², ZHANG Xiu-ying¹, WU Shu-zhen², YUAN Fei¹, WANG Hai-bo², XIAO Shi-he¹

1. Institute of Crop Science, Chinese Academy of Agriculture Science, Beijing 100081, China;
2. Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

Received:2010-06-19 Published:2010-08-28

摘要/Abstract

摘要： 为了培育抗穗发芽品种,对我国10个小麦主产省份的75个品种进行了穗发芽抗性鉴定,同时利用3个分子标记Vp1B3、Xbarc310和Barc294对上述品种进行了分子检测.结果表明,不同品种间穗发芽抗性存在明显差异.Vp1基因的等位变异与穗发芽抗性关系不密切,Vp1B3标记难以用于品种的穗发芽抗性筛选.主效QTL (QPhs-3AS)与种子休眠关系密切,Xbarc310和Barc294可作为穗发芽抗性选择的重要参考,且Barc294较Xbarc310的选择效果好.上述结果为抗穗发芽小麦品种的改良和推广提供了重要信息.

关键词: 小麦, 穗发芽, 分子标记

Abstract: Pre-harvest sprouting (PHS) in wheat is an important problem that results in significant economic loss to farmers. In this study，the PHS resistances of 76 Chinese wheat cultivars collected from ten wheat growing provinces were identified; Moreover，the distribution of three molecular markers Vp1B3，Xbarc310 and Barc294 associated with PHS resistance in the 75 cultivars was identified，aimed to evaluate the efficiency of these markers in selecting genotypes with higher PHS resistances. The results indicated that there is significant difference of PHS resistances in above wheat cultivars. The allelic variations of Vp1 gene are not associated with the PHS resistance; Vp1B3 was non-specific and unsuitable for marker assisted selection (MAS) in breeding. The 3As QTL (QPhs-3AS) is a critical component of seed dormancy，Xbarc310 and Barc294 were specific and suitable for MAS. The usefulness of current information in wheat breeding is discussed.

Key words: Wheat, Pre-harvest sprouting, Molecular marker

中图分类号:

孙果忠, 游光霞, 孙京燕, 张秀英, 武淑祯, 苑菲, 王海波, 肖世和. 我国小麦主推品种穗发芽抗性鉴定及相关分子标记的评价[J]. 华北农学报, 2010, 25(4): 6-11. doi: 10.7668/hbnxb.2010.04.002.

SUN Guo-zhong, YOU Guang-xia, SUN Jing-yan, ZHANG Xiu-ying, WU Shu-zhen, YUAN Fei, WANG Hai-bo, XIAO Shi-he. Identification on Pre-harvest Sprouting Resistance and Evaluation to Related Molecular Markers in Chinese Wheat Cultivars[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 6-11. doi: 10.7668/hbnxb.2010.04.002.

http://www.hbnxb.net/CN/Y2010/V25/I4/6

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Identification on Pre-harvest Sprouting Resistance and Evaluation to Related Molecular Markers in Chinese Wheat Cultivars

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Identification on Pre-harvest Sprouting Resistance and Evaluation to Related Molecular Markers in Chinese Wheat Cultivars

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