华北农学报 ›› 2010, Vol. 25 ›› Issue (3): 73-75. doi: 10.7668/hbnxb.2010.03.017

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绍兴鸭微卫星DNA遗传多样性分析

袁青妍1, 陶争荣1, 李国勤1, 沈军达1, 陈奕春2, 陈文标3, 王德前1, 虞永亮3, 田勇1, 原爱平1, 卢立志1   

  1. 1. 浙江省农业科学院, 畜牧兽医研究所, 浙江 杭州 310021;
    2. 缙云县畜牧兽医局, 浙江 缙云 321400;
    3. 浙江五莲农牧有限公司, 浙江 缙云 321403
  • 收稿日期:2009-12-23 出版日期:2010-06-28
  • 作者简介:袁青妍(1971-),女,陕西韩城人,博士,主要从事家禽分子遗传育种研究.
  • 基金资助:
    国家“十一五”科技支撑计划项目(2006BAD14B06);浙江省重大科技攻关项目(2005C12005-01);浙江省重大国际合作项目(2006C14015)

The DNA Genetic Diversity of Shaoxing Duck Populations

YUAN Qing-yan1, TAO Zheng-rong1, LI Guo-qin1, SHEN Jun-da1, CHEN Yi-chun2, CHEN Wen-biao3, WANG De-qian1, YU Yong-liang3, TIAN Yong1, YUAN Ai-ping1, LU Li-zhi1   

  1. 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agriculture Sciences, Hangzhou 310021, China;
    2. Jinyun Bureau of Animal Husbandry and Veterinary, Jinyun 321400, China;
    3. Wulian Agriculture and Livestock Corporation of Zhejiang, Jinyun 321403, China
  • Received:2009-12-23 Published:2010-06-28

摘要: 采用鸭的6个高度多态且分布在不同连锁群上的微卫星标记,对原种绍兴鸭种群中48个体和青壳II系绍兴鸭种群中34个体分别进行遗传多样性分析,共检测到152个等位基因,每个微卫星座位上等位基因数介于20~30,基因频率分布在0~0.2,在两个种群中每个微卫星标记的杂合度及多态信息含量均在0.8以上。原种绍兴鸭在3个座位上处于Hardy-Weinberg不平衡状态,而青壳II系绍兴鸭在2个座位上处于Hardy-Weinberg不平衡状态,而且它们都在AY493314座位处于Hardy-Weinberg不平衡状态。研究结果表明:原种绍兴鸭与青壳II系绍兴鸭群体内均存在丰富的遗传多样性。

关键词: 绍兴鸭, 遗传多样性, 微卫星标记

Abstract: Six microsatellite markers with high polymorphisms locating at different linkage groups were used to analyze the microsatellite DNA genetic polymorphisms of the 48 and 34 Shaoxing ducks sampled from element breed population and blue-eggshelled II strain population, respectively.A total of 152 alleles were detected, and the numbers of alleles at each locus varied from 20 to 30.The gene frequencies were 0 to 0.2.Both the hererozygosity( H) and polymorphic information content( PIC) were more than 0.8 at each microsatellite locus in the two populations.In the element breed population and blue-egg shelled II strain population, there were 3 and 2 loci, respectively,which were not in Hardy-Weinberg equilibrium, and they were all at AY493314 locus.The results revealed that the genetic variabilities in the element breed and blue-eggshelled II strain populations analysed were rich.

Key words: Shaoxing duck, Genetic diversity, Microsatellite DNA

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引用本文

袁青妍, 陶争荣, 李国勤, 沈军达, 陈奕春, 陈文标, 王德前, 虞永亮, 田勇, 原爱平, 卢立志. 绍兴鸭微卫星DNA遗传多样性分析[J]. 华北农学报, 2010, 25(3): 73-75. doi: 10.7668/hbnxb.2010.03.017.

YUAN Qing-yan, TAO Zheng-rong, LI Guo-qin, SHEN Jun-da, CHEN Yi-chun, CHEN Wen-biao, WANG De-qian, YU Yong-liang, TIAN Yong, YUAN Ai-ping, LU Li-zhi. The DNA Genetic Diversity of Shaoxing Duck Populations[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 73-75. doi: 10.7668/hbnxb.2010.03.017.