摘要： 采用半定量RT-PCR技术检测分析了在体外培养的猪成纤维细胞内分别添加终浓度为0.5,5,10,30μg/μL的维生素A后,分别作用12,24,48和72 h后,视黄酸受体γ基因(RARγ)的表达变化情况。结果表明,不同浓度组间差异不显著,但是分别作用24和72 h后,会出现抑制RARγ基因表达的现象。

关键词: 猪, 视黄酸受体&gamma, 基因, 成纤维细胞, RT-PCR

Abstract: In the present study ,RARγexpression was determined by RT -PCR in fibroblast cells cultured in additional 0.5,5,10,30 g/L vitamin A media and cultured 12,24,48 and 72 h respectively.The results showed that different concentration had no difference,but after affected 24 and 72 h respectively,it would appearing inhibition phenomenan.

Key words: Pig, Retinoicacidreceptorγ, Fibroblastcell, RT-PCR

中图分类号: 

• Q786