cDNA-AFLP技术及其在植物基因表达分析中的应用

doi:10.7668/hbnxb.2009.S1.005

摘要/Abstract

摘要： cDNA-AFLP是一种新的mRNA指纹图谱技术,具有重现性高、准确、可靠的特点,可对生物体转录组进行全面分析,广泛应用于基因表达特性研究、植物遗传标记分析和分离植物基因等方面。近年来随着该技术的不断发展和应用,在研究中取得了很多成果。本研究就cDNA-AFLP技术的原理和特点,在植物基因表达研究中的应用现状和发展前景进行了综述。

关键词: cDNA-AFLP, mRNA, 基因表达

Abstract: cDNA -AFLP is a new technique of fingerprinting of mRNA,and its high reproducibility,accuracy and reliability,so that it comprehensively analyse organism transcriptome,this technique is widely applied in gene expression research about analyzing differential expression,genetic marker analysis and identifying genes.With development and apply of this technique ,many achievement were obtain in research.The paper summarizes the principle,technical features,prospect and appropriate present situation of the technique.

Key words: cDNA-AFLP, mRNA, Geneexpression

中图分类号:

Q786

ZHAOJi-rong, LUOShu-zhen, ZHANGZeng-yan, LIUHong-xia. cDNA -AFLP and Its Application about Gene Expressions Analysis of Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.005.

http://www.hbnxb.net/CN/Y2009/V24/IS1/18

参考文献

[1] Liang P,Pardee A B.DiITerential display of eukaryotic messengerRNA by menas of the polymerase chain reaction[J].Science,1992,257 :967-971.
[2] 李子银,张劲松,陈受宜.水稻盐胁迫应答基因的克隆、表达及染色体定位[J].中国科学C 辑,1999,29(6):561-571.
[3] 王学德,朱英国.水稻雄性不育与可育花粉的mRNA 差异显小和cDNA 片段的分析[J].中国科学C 辑,1998 ,28(3):257 -263.
[4] Bachem C W B,Hoeven R S van der,Bruijn S M de,et al.Visualiza -tion of differential gene expression using a novelmethod of RNA fingerprinting based on AFLP:analysis of geneexpression during potato tuber development[J].Plant Journal ,1996,9 :745-753.
[5] 付凤玲,李晚忱.用cDNA -AFLP 技术构建基因组转录图谱[J].分子植物育种,2003,1(4):523 -529.
[6] 卢　钢,曹家树.cDNA-AFLP 技术在植物表达分析上的应用[J].植物学通报,2002,19(1):103 -108.
[7] 姜立杰,张开春,张晓明.cDNA-AFLP 技术及其在基因表达中的应用[J].中国生物工程杂志,2003,23(12):82-86.
[8] Bachem C W B,Oomen R J F J,Visser R G F.Transcriptimagjng with cDNA -AFLP:a step -by -step protocol[J].Plant Mnlecu-lar Biology Reporter,1998,16:157 -173.
[9] 王　锋.cDNA-AFLP 技术原理及其在研究植物基因差异表达中的应用[J].生物学通报,2005(40)7 :59-60.
[10] Money T,Reader S,Qu L J,et al.AFLP-based mRNA fingerprinting[J].Nucleic Acids Resemch,1996,24:2616 -2617.增刊赵继荣等:cDNA -AFLP 技术及其在植物基因表达分析中的应用 2　1
[11] Habu Y,Fukada -Tanaka S,Hisatomi Y,et al,Amplifiedrestriction fragment length polymrphisnr based mRNA fingerprint-ing using a single restriction enzyme that recognizes a4b psequence[J].Birxhemical and Binph3zsical ResearchCommunications,1997,234:516-521.
[12] Donson J,Fang Y,Espiritu Santo G.et al.Comprehensivegeneexpression analysis by transcript profiling [J].PlantMolecular Biology,2002,48:75 -97.
[13] 吴敏生,高志环,戴景瑞.利用cDNA-AFLP 技术研究玉米基因的差异表达[J].作物学报,2002,27(3):339-342.
[14] Tian Zengyuan,DAI Jingrui.Relationship between differentialgene expression pat-terns in functional leaves of maize(Zea mays L.)at milk filling stage and heterosis using cDNA-AFLP(英文)[J].中国科学通报,2003,48(1):76-81.
[15] Tian Zengyuan,DAI Jingrui.Relationship between differentialgene expression patterns in functional leaves 　of maizeinbreds & hybrids at spikelet differentiation stage and heterosis(英文)[J].遗传学报,2003,30(2):154-162.
[16] 吴才君,曹家树,董德坤.芸薹种蔬菜杂交种及其亲本莲座期基因差异表达与杂种优势的关系[J].中国农业科学,2004,37(11):1654-1659.
[17] Zheng Xian -wu,Chen Xue -wei,Zhang Xiao -hong,etal.Isolation and identification of a gene in response to riceblast disease in rice[J].Plant Mol Biology,2004,54:99 -109.
[18] Tatiana Suprunova,Tamar krugman,Assaf Distelfeld,et al.Identification of a novel gene(hsdr4) involved in water -stress tolerance in wild barly.[J].PlantMol Biology,2007,64 :17-34.
[19] Brugmans B,Carmen A F D,Bachem C W B,et al.A novelmethod for the construction of genome wide transcriptomemaps[J].The Plant Journal,2002,31(2):211 -222.
[20] Luo M,Jia JZ.Progress in expressed sequence tags (EST)project of plant genome[J].Progress in Biochemistry andBiophysics,2001,28:494-497.
[21] Boventius H ,WellerJ I.Mapping and analysis of dairy cattlequantitative trait loci by maximum likelikehood methodologyusing milk protein gene as genetic markers[J].Genetics,1994,137 :267-280.
[22] Suhrez M C,Bernal A,GutiErrez J,et al.Developing expressedsequence tags(ESTs)from polymorphic transcript -derived fragments(TDFs) in cassava (Manihot esculentaCrantz)[J].Genome,2000,43:62 -67.
[23] Bart B1,Asun Fldel C1,Christian Wl,et al.A novel methodfor the construction of genome wide tran scriptome maps[J],Plant,2002,31(2):211 -222.
[24] Kehoe D M,Pomerville S.DNA microarrays for studies ofhigher plants and other phntosynthethic organisms [J].Trends Plant Sci,1999,4 :38-41.
[25] Terryn N,Kouze P,Montagu M V.Plant genomics[J].FEBSLetters,1999,452:3-6.
[26] 肖月华,罗　明,韦宇拓,等.棉花纤维起始期基因表达的cDNA -AFLP 分析[J].农业生物技术学报,2003,11(1):20-24.
[27] Huang J,Bachem C,Jacobsen E,et al,Molecular analysis ofdifferentially expressed genes during postharvest deteriorationin cassava(Manihot esculenta Crantz)tuberous roots[J].Euphytica,2001,120:85 -93.
[28] Juliette Leymarie,Elisabeth Bruneaux,Stephanie Gibot -Leclerc,et al.Identification of transcripts potentially involvedin barley seed germination and dormancy using cdna-aflp[J].Journal of Experimental Botany,2007,58(3) :425-437.
[29] Chuanzao Mao,Keke Yi,Ling Yang,et al.Identification ofaluminium -regulated genes by cDNA -AFLP in rice(Oryzasativa L.):aluminium - regulated genes for themetabolism of cell wall components[J].Journal of ExperimentalBotany,2004,55(394):137-143.
[30] Habu Y,Fukada -Tanaka S,Hisatomi Y,et al.Amplifiedrestriction fragment length polymrphism -based mRNA fingerprint-ing using a single restriction enzyme than recognizesa 4 -bp sequence[J].Biochemical and BiophysicalResearch Communications,1997,234:516 -521.
[31] 侯　磊,肖月华,李先碧,等.棉花洞A 雄性不育系花药发育的mRNA 差别显示[J].遗传学报,2002,29(4):359-363.
[32] 李德谋,肖月华,罗明,等.棉花PTS2 受体基因(GhPex7)的克隆及表达分析[J].遗传学报,2003,30(9):823 -829.
[33] 陈桂平,黄占景,马闻师,等.盐胁迫下小麦耐盐突变体后代差异cDNA 的分离和鉴定[J].中国农业科学,2003,36(9):996-999.
[34] 陈桂平,马闻师,黄占景,等.小麦耐盐突变休盐胁迫下SIR73 基因片段的分离和鉴定[J].遗传,2003,25(2):173 -176.
[35] Gui Ping Chen ,Wen Shi Ma,Zhan Jing Huang,et al.Isolationand characterization of TaGSKl involved in wheat salttolerance[J].Plant Science,2003,165:1369-1375.
[36] Christina Eckey,Michael Korell,Katja Leib,et al.Identificationof powdery mildew induced barley genes by cDNAAFLP:functional assessment of an early expressed MAP kinase[J].Plant Molecular Biology,2004,55:1 -15.
[37] 郭　军,屈冬玉,王晓武,等.cDNA -AFLP 结合BSA研究马铃薯晚疫病菌小种特异无毒基因差异表达片段[J].中国马铃薯,2004,18(1) :1-3.
[38] Gabriels S H,Takken F L,Vossen J H,et al,cDNA-AFLPcombined with functional analysis reveals novel genes involvedin the hypersensitive response[J].Molecular Plant -Microbe Interaction,2006,19(6):567-76.
[39] Johnes J T,Rower H.A comparison the efficiency of differentialdisplay and cDNA-AFLPs as a tools for the isolationof differ -entially expressed parasite genes[J].Fundamentaland Applied Nemadity,1998,21:81-88.
[40] Vanderbiezen E A,Juwana H,Parker J E,et al.cDNA -AFLP display for the isolation of Peronospora -Parasiticagenes expressed during infection in Arabidapsis thaliana[J].Molecular Plant -Microbe Interactions,2000,13(8):895 -89.
[41] Parniske M.Intracellular accommodation of microbes byplants:a common developmental program for symbiosis anddisease[J].Current Opinion in Plant Biology,2000,3:320-328.
[42] Besse P,Taylor G,Carroll B,et al.Assessing genetic diversityin a Sugarcane germplasm collection using an automatedAFLP analysis[J].Genetics,1998,104 :143-153.

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