华北农学报 ›› 2009, Vol. 24 ›› Issue (4): 188-192. doi: 10.7668/hbnxb.2009.04.037

所属专题: 甜瓜 蔬菜专题

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HrpNEa诱导甜瓜对蚜虫趋避作用的研究

苟振华, 张书萍, 董汉松   

  1. 南京农业大学, 农业部病虫监测与治理重点开放实验室, 江苏南京 210095
  • 收稿日期:2009-05-07 出版日期:2009-08-28
  • 作者简介:苟振华(1982-),男,山东青岛人,硕士,主要从事植物抗病信号传导研究.
  • 基金资助:
    国家自然科学基金(0771441)

Effects of HrpNEa on Inducing Aphid Repellency on Cucumis melo

GOU Zhen-hua, ZHANG Shu-ping, DONG Han-song   

  1. Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-05-07 Published:2009-08-28

摘要: 通过检测甜瓜中与拟南芥韧皮部特异性表达蛋白的编码基因AtPP2相类似的基因(AtPP2-like)的表达情况,以及甜瓜植株的胼胝质沉积效应,证明了这二者与甜瓜植株对蚜虫抗性之间的联系.利用反转录PCR (RT-PCR)的方法,检测了甜瓜两个AtPP2类似蛋白的编码基因lec17和lec26,受到HrpNEa的诱导表达增强,同时通过苯胺蓝染色鉴定了胼胝质在HrpNEa处理的甜瓜植株中沉积增加.接种蚜虫后的表型观测结果表明HrpNEa处理的甜瓜生长加快,抗虫效应增加.口针刺吸活动的电波信号图(Electrical penetration graph,EPG)结果显示,HrpNEa处理的甜瓜与对照相比蚜虫的取食活动明显减少,刺探性活动增加,取食频率大幅降低.这些结果表明HrpNEa处理增强了甜瓜对蚜虫的抗性.

关键词: HrpNEa, 甜瓜, 蚜虫, 趋避效应

Abstract: :It has been reported that HrpNEa could induce the insect resistance of Arabidopsis. Here we demonstrated that HrpNEa also showed potential to enhance the aphid repellency on Cucumis melo. The expression of AtPP22like gene ( lec17 and lec26) in Cucumis melo determined by RT2PCR and the callose deposition in phloem measured by aniline blue staining were both induced by HrpNEa. The action of aphids after placed on Cucumis melo indicated that HrpNEa could in2 duce insect resistance on Cucumis melo. EPG (electrical penetration graph) results revealed that HrpNEa could decrease the feeding frequency of aphids. Thus,we draw the conclusion that HrpNEa could enhance the resistance of Cucumis melo to aphids.

Key words: Cucumis melo, Aphid, Repellance

中图分类号: 

引用本文

苟振华, 张书萍, 董汉松. HrpNEa诱导甜瓜对蚜虫趋避作用的研究[J]. 华北农学报, 2009, 24(4): 188-192. doi: 10.7668/hbnxb.2009.04.037.

GOU Zhen-hua, ZHANG Shu-ping, DONG Han-song. Effects of HrpNEa on Inducing Aphid Repellency on Cucumis melo[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 188-192. doi: 10.7668/hbnxb.2009.04.037.