摘要： 小麦接种高度非亲和性的小麦秆锈菌后,在其细胞间隙液(IWF)中可能存在激发子。通过硫酸铵沉淀法将其分级,再将各分级沉淀蛋白溶液注射到高度非亲和性互作小麦叶片中,观察过敏性坏死反应(HR)和过氧化物酶(POD)及苯丙氨酸解氨酶(PAL)活性变化。结果表明:激发子成分主要集中在50%~80%硫酸铵沉淀蛋白部分中,能明显诱导叶片发生过敏性坏死反应 40μg/mL的该分级沉淀蛋白溶液处理小麦叶片后,PAL和POD活性在早期已有显著增加,分别在诱导处理后36 h和60 h左右出现最高峰,较对照分别增加了37.47%和78.20%。

关键词: 小麦, 激发子, 过敏性坏死反应, 过氧化物酶, 苯丙氨酸解氨酶

Abstract: Elicitors can be found in intercellular washing fluids(IWFs) in highly incompatible interaction betweenwheat and stem rust. The IWFs were classified using Ammonium Sulfate Precipitation and all the proteins dissolved inddH₂0₂ were injected into highly incompatible interaction wheat leaves respectively,then hypersensitive response was ob-served and activity dynamics of peroxidase(POD) and phenylalanine ammonia-lyase(PAL) were measured. The resultshowed that elicitor (s) mainly was (were) included in the 50%-80%classification and obviously induced the hypersensi-five response of wheat leaves. POD and PAL activity had remarkable increase after inducement by 40μg/ mL protein solu-tion of 50%-80%classification. POD activity reached highest point at the time of 36 h and had 37. 47%increase,andthat of PAL activity were 60 h and 78. 20%.

Key words: Wheat, Elicitor, Hypersensitive response, Peroxidase, Phenylalanine ammonia-lyase

中图分类号: 

• S435.121