摘要： 根据GenBank中山羊SRY基因序列设计一对引物，采用PCR在雄性辽宁绒山羊个体中扩增出了包含SRY基因整个编码区的特异片段，将特异PCR产物克隆到pMD18-T 载体中，转化DHα菌，筛选出SRY基因的阳性克隆，进行了测序，将其序列与GenBank中部分偶蹄目动物的SRY基因序列进行同源性比较，用NJ法构建了系统进化树。结果表明，辽宁绒山羊SRY基因编码区长度为723 bp，共编码240个氨基酸，其中包含长度为231 bp 的HMG-box，位于编码区的第190至420位核苷酸之间，编码77个氨基酸(H⁶⁴-A¹⁴⁰)。辽宁绒山羊SRY基因编码区序列与GenBank中山羊、绵羊、牛、牦牛、水牛、梅花鹿、麝香鹿及猪等偶蹄目动物的同源性分别为100%，97.09%，89.21%，89.21%，88.38%，87.%，86.16%和68.04%；基于SRY基因编码区的核苷酸序列，构建的物种间系统树结果基本上与这些偶蹄目动物的传统系统分类相一致。

关键词: 辽宁绒山羊, SRY基因, 编码区, 序列分析

Abstract: The fragment ofSRYgene containing entire encoding region from Liaoning cashmere goat genomic DNA was amplified by the polymerase chain reaction(PCR)with a pair of designed primers based on the sequence of goatSRYgene sequence from GenBank.The amplified fragment was cloned to the pMD18-T vector,and transformed to the bacteria DH5α.The identified positive clone with Liaoning cashmere goatSRYgene fragment was sequenced.The sequences iden-tities ofSRYgene encoding region of some artiodactyla from GenBank were compared with the Liaoning cashmere goatSRYgene fragment,and the phylogenetic tree was constructed by NJ method.The results are as follows.The entire coding region of Liaoning cashmere goatSRYgene is 723 bp,encoding a peptide with 240 amino acid residues,and it contains the HMG-box with 231 bp between position 190 and 420,encoding 77 amino acid residues(H⁶⁴-A¹⁴⁰).The corresponding nucleotide sequences in GenBank of goat,sheep,cattle,yak,water buffalo,sika deer,musk deer,and pig had identities of 100%,97.09%,89.21%,89.21%,88.38%,87.55%,86.16% and 68.04%,respectively,with the Liaoning cash-mere goat.The clustering based on the sequences ofSRYgene encoding region among species is generally in agreement with the classic taxonomic relationship.

Key words: Liaoning cashmere goat, SRYgene, Encoding region, Sequence analysis

中图分类号: 

• S827