华北农学报 ›› 2008, Vol. 23 ›› Issue (4): 69-71. doi: 10.7668/hbnxb.2008.04.016

所属专题: 畜牧 生物技术

• 论文 • 上一篇    下一篇

猪GPX5基因、FUT1基因和NCOA1基因的多态性分析

赵金凤1, 张冬杰2, 谷溪1, 谢雨彤1, 刘娣1,2   

  1. 1. 东北农业大学 动物科学技术学院,黑龙江 哈尔滨 150030;
    2. 黑龙江省农业科学院,黑龙江 哈尔滨 150086
  • 收稿日期:2008-03-12 出版日期:2008-08-28
  • 通讯作者: 刘娣(1963-),女,黑龙江哈尔滨人,博士生导师,主要从事动物遗传育种与繁殖研究
  • 作者简介:赵金凤(1984-),女,黑龙江哈尔滨人.在读硕士,主要从事动物遗传育种与繁殖研究

Analysis of Polymorphism on GPX5,FUT1 and NCOA1 Gene in Swine(Pig)

ZHAO Jin-feng1, ZHANG Dong-Jie2, GU Xi1, XIE Yu-tong1, LIU Di1,2   

  1. 1. College of Animal Science and Technology,North East Agricultural University,Harbin 150030,China;
    2. Heilongjiang Academy of Agriculture Sciences,Harbin 150086,China
  • Received:2008-03-12 Published:2008-08-28

摘要: 采用PCR-RFLP法对大白猪、杜洛克、长白猪、民猪和野家杂交猪的谷胱甘肽过氧化物酶5(Glutathion-Peroxi-dase 5,GPX5)基因、岩藻糖转移酶1(Fucosyltransferase1,FUT1)基因和核受体辅激活蛋白1(Nuclear receptor co-activator 1,NCOA1)基因进行多态性检测,结果表明:GPX5基因用HinfⅠ酶切可获得3种基因型,FUT1基因用HhaⅠ酶切可获得3种基因型,NCOA1基因用RsaⅠ酶切可获得3种基因型。同时对不同基因的基因型频率和基因频率进行计算,并分析相应基因的遗传效应。

关键词: 猪, GPX5基因, FUT1基因, NCOA1基因, PCR-RFLP

Abstract: For G lutathion-Peroxidase 5 ( GPX5 ),Fucosyltransferasel ( FUT1 ) and Nuclear receptor co-activator 1 ( NCOA 1) gene,the target segment of those genes were amplified through PCR,and then these amplified segments in five strains of pig were genotyped by PCR-RFLP 1 The strains involved were Landrace Y orkshire,Duroc,Min pig and Crossbred pig.After genotyping by PCR-RFLP with Hinf lin GPX5 gene obtained 3 genotypes;With Hha lin FUT1 gene ob-tained 3 genotypes;With Rsa lin NCOA1 gene obtained 3 genotypes 1 Then the frequency of gene and genotype of these genes were calculated,and analysised for genetic effect.

Key words: Swine, GPX5 gene, FUT1 gene, NCOA1 gene, PCR-RFLP

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引用本文

赵金凤, 张冬杰, 谷溪, 谢雨彤, 刘娣. 猪GPX5基因、FUT1基因和NCOA1基因的多态性分析[J]. 华北农学报, 2008, 23(4): 69-71. doi: 10.7668/hbnxb.2008.04.016.

ZHAO Jin-feng, ZHANG Dong-Jie, GU Xi, XIE Yu-tong, LIU Di. Analysis of Polymorphism on GPX5,FUT1 and NCOA1 Gene in Swine(Pig)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 69-71. doi: 10.7668/hbnxb.2008.04.016.

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