摘要： 为了筛选出对番茄溃疡病菌有拮抗作用的内生细菌,从健康番茄茎样本中分离得到186株内生细菌。用抑菌圈法测定,其中9株内生细菌对番茄溃疡病菌有拮抗作用。通过形态特征观察、生理生化特性测定和16 S rDNA序列分析,对拮抗作用较强的7个菌株进行了鉴定。结果表明,BM-31、BT-10、BT-9、BT-7、BT-3为微杆菌(Microbacterium spp.),QB-22和QB-8为假单胞菌(Pseudomonas spp.)。
关键词:
番茄,
内生细菌,
溃疡病菌,
拮抗作用
Abstract: For filtrating endophytic bacteria and its inhibition on Clavbacter michiganensis subsp. michiganensis, one hundred and eighty -six endophytic bacterium strains were isolated from the stems of healthy tomato plants collected from some cities( counties) of Inner Mongolia at the fruiting stage1According to the test of inhibiting the growth of Clavbacter michiganensis subsp. michiganensis, seven strains have antagonistic activity against the plant pathogen1The results showed that BM -31, BM -18, BM -14, BT -10, BT -9, BT -7, BT -3were classified as Microbacterium spp. ;QB -22,QB -8 were class- i fied as Pseudomonas spp. according to them morphological, physiological and biochemical characteristics and 16 S rDNA sequence analysis.
Key words:
Tomato,
Endophytic bacterial,
Clavbacter michiganensis subsp. michiganensis,
Inhibition
中图分类号:
袁亮, 胡俊, 高润蕾, 苏高升. 番茄内生细菌对番茄溃疡病菌拮抗作用的研究[J]. 华北农学报, 2008, 23(4): 41-44. doi: 10.7668/hbnxb.2008.04.010.
YUAN Liang, HU Jun, GAO Run-lei, SU Gao-sheng. Identification of Endophytic Bacteria in Tomato Plant and Its Inhibition on Clavbacter michiganensis subsp.michiganensis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 41-44. doi: 10.7668/hbnxb.2008.04.010.