华北农学报 ›› 2007, Vol. 22 ›› Issue (6): 50-52. doi: 10.7668/hbnxb.2007.06.010

所属专题: 小麦

• 论文 • 上一篇    下一篇

小麦微卫星标记在中间偃麦草中通用性研究

王黎明1, 李兴锋2, 刘树兵2, 王洪刚2   

  1. 1. 河南科技大学, 农学院, 河南, 洛阳, 471003;
    2. 山东农业大学, 农学院, 国家小麦改良分中心, 作物生物学国家重点实验室, 山东, 泰安, 271018
  • 收稿日期:2007-08-08 出版日期:2007-12-28
  • 通讯作者: 王洪刚(1955-), 男, 山东滕州人, 博士, 教授, 博士生导师, 主要从事现代生物技术与作物遗传改良研究工作
  • 作者简介:王黎明(1976-), 男, 山东日照人, 讲师, 博士, 主要从事作物遗传改良的研究工作
  • 基金资助:
    国家自然科学基金项目(30571156, 30671300); 河南科技大学校基金项目(2007ZY017); 河南科技大学博士基金项目(09001130)

Studies on the Transferability of Common Wheat(T. Aestivum) Microsatellites(SSR)Markers Used in Thinopyrum intermedium

WANG Li-ming1, LI Xing-feng2, LIU Shu-bing2, WANG Hong-gang2   

  1. 1. Agronomy College of Henan University of Science and Technology, Luoyang 471003, China;
    2. Agronomy College of Shandong Agricultural University, Subcentre of National Wheat Improvement Centre, State Key Laboratory of Crop Biology, Tai'an 271018, China
  • Received:2007-08-08 Published:2007-12-28

摘要: 利用分布于普通小麦整个基因组的525对微卫星引物, 对其在普通小麦与中间偃麦草(Thinopyrum intermedi-um)之间的通用性及其亲缘关系进行了分析。结果表明: 202对引物在小麦和中间偃麦草间多态性扩增, 所占比率为38.4%, 小麦的A, B, D3个基因组多态性引物所占比率分别为34.6%, 36.9%和42.2%; 说明普通小麦SSR引物在中间偃麦草之间具有通用性, 小麦D基因组与中间偃麦草亲缘关系要远于A, B基因组与中间偃麦草关系, 表明小麦的A, B, D基因组之间存在遗传差异性。

关键词: 小麦, SSR标记, 中间偃麦草, 通用性, 遗传差异

Abstract: 525 pairs of common wheat SSRs primers distributed in the total genomes of T. aestivum were used to identify the transferability in Thanopyrum intermedaum and to analyze the genetic relationship between T. aestivum Thinopyrum intermedaum.The results were、follows: 202 pairs SSRs primers amplified polymorphism between T.aestivum and Thancpyrum intermedium, which polymorphic percentage was 38.4%.And the polymorphic is percentage between T. aestivum on genome A, B, D and Thanopyrum intermedaum was 34.6%, 36.9% and 42.2% respectively.These resups showed that wheat SSRs pruners can be used in Tlanopyrum intermedaum; the genetic relationship between T. aestiwum on genome D and Thancpyrum intermedaum was far than that of on genome A, B, which indicated that therewas genetic differentiation within T. aestivum genome A, B, and D.

Key words: T. aestivum, SSR, Thincpyrum interniedium, Transferability, Genetic differentiation

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引用本文

王黎明, 李兴锋, 刘树兵, 王洪刚. 小麦微卫星标记在中间偃麦草中通用性研究[J]. 华北农学报, 2007, 22(6): 50-52. doi: 10.7668/hbnxb.2007.06.010.

WANG Li-ming, LI Xing-feng, LIU Shu-bing, WANG Hong-gang. Studies on the Transferability of Common Wheat(T. Aestivum) Microsatellites(SSR)Markers Used in Thinopyrum intermedium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 50-52. doi: 10.7668/hbnxb.2007.06.010.

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