摘要： CACNA2D1基因定位在猪的第9号染色体上，其位置与影响肉质性状的QTL重叠，可作为肉质性状的候选基因。采用PCR-SSCP技术，对160头金华×皮特兰资源家系F2猪CACNA2D1基因3’端非翻译区片段进行扩增分型，共产生2种等位基因，3种基因型。通过测序发现，在CACNA2D1基因3’端非翻译区(序列DQ981407)有一单核苷酸替换(C→A)。对不同标记基因型与肉质性状的最小二乘分析表明，AA基因型猪大腿肌肉的pH4值、大腿肌肉导电率和肌内脂肪含量的最小二乘均数比BB基因型分别高0.169，0.26和0.021，差异显著(P<0.0)；A等位基因的加性效应分别为0.084，0.133 和0.010。

关键词: 猪, CACNA2D1基因, PCR-SSCP, 肉质

Abstract: CACNA2D1 could be a candidate gene for meat quality traits because it has been mapped to porcine chromosome 9 and many QTLs influencing meat quality traits have been also detected in this region. In this research, 160 individuals from the F2 generation of Jinhua×Pietrain resource family had been genotyped on the locus of 3' UTR of CACNA2D1 gene, and 2 alleles, 3 genotypes had been found by PCR-SSCP. One single nucleotide substitution (C→A) at the position of 3' UTR of CACNA2D1 gene (GenBank accession No: DQ981407) was identified by sequencing. The relationship among different genotype from meat quality traits was studied by the least square analysis. The result showed that the least square means of ham muscle pH45 value, ham muscle electric conductivity and eye muscle crude lipid content relative quantity in AA genotype is significant higher than that of BB genotype by 0.169, 0.26 and 0.021 (P<0.05) and the additive effect of A allele is 0.0845, 0.1335 and 0.0105 respectively.

Key words: Pig, CACNA2D1 gene, PCR-SSCP, Meat quality

中图分类号: 

• S828.03