酶解酪蛋白法测定猪回肠内源性氨基酸排泄量的研究

doi:10.3321/j.issn:1000-7091.2006.z1.043

华北农学报 ›› 2006, Vol. 21 ›› Issue (S1): 181-185. doi: 10.3321/j.issn:1000-7091.2006.z1.043

所属专题： 畜牧；

酶解酪蛋白法测定猪回肠内源性氨基酸排泄量的研究

李美香^1,2, 李铁军¹, 宾石玉^1,3, 印遇龙¹, 黄瑞林¹, 伍国耀¹

1. 中国科学院亚热带农业生态研究所亚热带农业生态重点实验室, 湖南长沙 410125;
2. 中国科学院研究生院, 北京 100039;
3. 广西师范大学生命科学院, 广西桂林 541000

收稿日期:2006-05-12 出版日期:2006-12-31
作者简介:李美香(1982-)，女，福建福州人，硕士研究生，主要从事动物生态营养研究；李铁军为通讯作者。
基金资助:
国家“973”项目(2004CB117502);国家自然科学基金(30371038;30528006)中科院知识创新主要方向项目(KSCX2-SW-323);中国科学院海外杰出学者基金项目(2005-1-4)

Study on Enzyme-hydrolysed Casein Method for Determining Endogenous Ileal Amino Acid Excretion in the Pig

LI Mei-xiang^1,2, LI Tie-jun¹, BIN Shi-yu^1,3, YIN Yu-long¹, HUANG Rui-lin¹, WU Guo-yao¹

1. Key Laboratory of Subtropical Agro-ecology, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, China;
2. The Graduate School of the Chinese Academy of Sciences, Beijing 100039, China;
3. College of Life Science, Guangxi Normal University, Guilin 541000, China

Received:2006-05-12 Published:2006-12-31

摘要/Abstract

摘要： 分别从超滤管用于酶解酪蛋白测定的可行性、不同规格超滤管对酶解酪蛋白测定的影响、酶解酪蛋白的适宜添加量、酶解酪蛋白应用的动物试验步骤以及酶解酪蛋白测定效果等方面，对酶解酪蛋白方法测定猪回肠内源性氨基酸排泄量进行了综述。

关键词: 内源性氨基酸, 酶解酪蛋白, 超滤管, 猪

Abstract: In this paper, it was summarized that effectiveness of an ultrafiltration device, different sizes of an ultrafiltration device, the concentration of dietary enzyme-hydrolysed casein, animal trial process and results of using enzyme-hydrolysed casein, respectively, on enzyme-hydrolysed casein method for determining endogenous ileal amino acid excretion in the pig.

Key words: Endogenous amino acid, Enzyme-hydrolysed casein, Ultrafiltration device, Pig

中图分类号:

S828

李美香, 李铁军, 宾石玉, 印遇龙, 黄瑞林, 伍国耀. 酶解酪蛋白法测定猪回肠内源性氨基酸排泄量的研究[J]. 华北农学报, 2006, 21(S1): 181-185. doi: 10.3321/j.issn:1000-7091.2006.z1.043.

LI Mei-xiang, LI Tie-jun, BIN Shi-yu, YIN Yu-long, HUANG Rui-lin, WU Guo-yao. Study on Enzyme-hydrolysed Casein Method for Determining Endogenous Ileal Amino Acid Excretion in the Pig[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(S1): 181-185. doi: 10.3321/j.issn:1000-7091.2006.z1.043.

http://www.hbnxb.net/CN/Y2006/V21/IS1/181

参考文献

[1] Moughan P J, Darragh A J, Smith W C, et al. Perehloric and trichloroacetic acids as precipitants of protein in endogenous ileal digesta from the rat[J]. J Sci Food Agric, 1990, 52: 13-21.
[2] CENTRIPREP Centrifugal Filter Devices User Guide[Z]. Millipore Corporation (USA). 2004, 1-27.
[3] Hodgkinson S M, Moughan P J. Effectiveness of an ultrafiltration device for use with the enzyme-hydrolysed protein method for determining endogenous ileal nitrogen and amino acid excretion in pig[J]. J Sci Food Agric, 2001, 81: 1592-1596.
[4] Hodgkinson S M, Moughan P J, Gordon W R, et al. The effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig[J]. British Journal of Nutrition, 2000, 83: 421-430.
[5] Hodgkinson S M, Moughan P J. The enzyme hydrolysed protein method for the determination of endogenous ileal nitrogen and amino acid flows-a modification[J]. Animal Feed Science and Technology, 2003, 108: 207-214.
[6] Moughan P J, Schuttert G, Leenaars M. Endogenous amino acid flow in the stomach and small intestine of the young growing pig[J]. Journal of the Science of Food and Agriculture, 1992, 60: 437-442.
[7] Butts C A, Moughan P J, Smith W C, et al. Endogenous lysine and other amino acid flows at the terminal ileum of the growing pig (20 kg bodyweight): the effect of protein-free, synthetic amino acid, peptide and protein alimentation[J]. Journal of the Science of Food and Agriculture, 1993, 61: 31-40.
[8] Butts C A, Moughan P J, Smith W C, et al. The effect of food dry matter intake on endogenous ileal amino acid excretion determined under peptide alimentation in the 50 kg liveweight pig[J]. Journal of the Science of Food and Agriculture, 1993, 62: 235-243.
[9] Schulze H, Butts C A, Moughan P J, et al. The15N-isotope dilution method for determining ileal endogenous nitrogen excretion in the young (10 kg liveweight) pig[J]. Journal of the Science of Food and Agriculture, 1995, 69: 41-50.
[10] 印遇龙. 猪回肠-盲肠"桥"式瘘管技术与回肠末端"T"型瘘管的比较实验[J]. 中国农业科学, 1989, 5: 81-85.
[11] Fan M Z, Li T J, Yin Y L, et al. Effect of phytase supplementation with two levels of phosphorus diets on ileal and faecal digestibilities of nutrients and phosphorus, calcium, nitrogen and energy balances in growing pigs[J]. Animal Science, 2005, 81: 67-75.
[12] National Research Council, Nutrient Requirements of Swine[S], 10th Edition (Revised). 1998.
[13] Lange C F M, Sauer W C, Mosenthin R, et al. The effect of feeding different protein-free diets on the recovery and amino acid composition of endogenous protein collected from the distal ileum and feces in pigs[J]. J Anim Sci, 1989, 67: 746-754.
[14] Fenton T W, Fenton M. An improved procedure for the determination of chromic oxide in feed and feces[J]. Can J Anim Sci, 1979, 59: 631-634.
[15] Mason V C, Bech-Andersen S, Rudemo M. Hydrolysate preparation for amino acid determinations in feed constituents[J]. J Anim Physiol Anim Nutr, 1980, 43: 146-164.
[16] Butts C A, Moughan P J, Smith W C. Protein nitrogen, peptide nitrogen and free amino acid nitrogen in endogenous digesta nitrogen at the terminal ileum of the rat[J]. Journal of the Science of Food and Agriculture, 1992, 59: 291-298.
[17] Leterme P, P van Leeumen, Thewis A J Huisman, et al. Determination of the true ileal digestibility of pea amino acid by means of15N-labeled diets or animals[A]. In: Souffrant W B, Hagenmeister H. Proceedings of the VIth International Symposium on Digestive Physiology in Pigs[C]. EAAP Publication no. 80, Bad Doberan. Germany, 1994: 21-24.
[18] Leterme P, Monmart T, Morandi P, et al. Effect of oral and parenteral N nutrition vs N-free nutrition on the endogenous amino acid flow at the ileum of the pigs[A]. In: Souffrant W B, Hagenmeister H. Proceedings of the VIth International Symposium on Digestive Physiology in Pigs[C]. EAAP Publication no. 80, Bad Doberan. Germany, 1994: 31-34.
[19] Butts C A, Moughan P J, Smith W C. Endogenous amino acid flow at the terminal ileum of the rat determined under condition of peptide alimentation[J]. J Sci Food Agric, 1991, 52: 179-192.
[20] Schulze H, Butts C A, Moughan P J, et al. Endogenous ileal nitrogen flow in pigs determined using the15N-isotope dilution and peptide alimentation ultrafiltration methods[A]. In: Souffrant W B, Hagenmeister H. Proceedings of the VIth International Symposium on Digestive Physiology in Pigs[C]. EAAP Publication no. 80, Bad Doberan. Germany, 1994: 14-16.
[21] Yin Y L, Mccracken K J. Methological aspects of the in vivo measurement of ileal amino acid digestibility in pigs: a review[J]. Asia J Anim Sci, 1996, 9: 495-502.

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酶解酪蛋白法测定猪回肠内源性氨基酸排泄量的研究

Study on Enzyme-hydrolysed Casein Method for Determining Endogenous Ileal Amino Acid Excretion in the Pig

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酶解酪蛋白法测定猪回肠内源性氨基酸排泄量的研究

Study on Enzyme-hydrolysed Casein Method for Determining Endogenous Ileal Amino Acid Excretion in the Pig

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