摘要： 以小麦品种东农7742的基因组DNA为模板，用PCR的方法克隆了小麦贮藏蛋白中高分子量谷蛋白12亚基基因（HMW-GS12）的上游调控序列。序列测定结果表明：所克隆的启动子片段大小为917 bp，该片段的498~922 bp序列与Thomspon报道的同一亚基对应区段序列比较，同源性为97.9%，有9个核苷酸发生了改变。推测的TATA box位于836~841 bp，有3个CAAT-like box，分别是693 bp处的CCAA；730 bp处的CAAAT和808 bp处的CCAT。另外，在684 bp存在E-box（TGCAAAG），还有2个E-like box，分别是608 bp处的TGCAAAC和510 bp处的TGCAAAA。认为该元件可能与转录速率和胚乳特异性的表达调控有关。

关键词: 小麦, 高分子量谷蛋白, 上游调控序列, 克隆

Abstract: The specific primers were designed according to the published sequence of wheat glutenin gene.The upstream regulatory region of a high molecular weight glutenin 12 subunit gene was amplified from wheat cultivar NE7742 genomic DNA by polymerase chain reaction.Sequencing analysis showed the cloned fragment contained 917 nucleotides and which located in 498-922 bp region shared a homology of 97.9% with the sequence published on genbank.There are 9 bp differences when compared with the reported sequence.The putative TATA box was present at position 836 to 841 bp and three possible CAAT-like boxes in 693 bp,730 bp and 808 bp respectively.A putative E-box and two E-likE-boxes were found.It was speculated that the presence of E-box were in responsible of gene endosperm-specific expression.

Key words: Wheat, HMW glutenin, Upstream regulatory region, Cloning

中图分类号: 

• Q33