摘要： 以麝香百合叶片愈伤组织为受体，利用根瘤农杆菌介导法将美洲商陆蛋白（PAP）基因和抗卡那霉素筛选基因以共转化的方式转入百合叶片愈伤组织中，然后在含有MS培养基中筛选愈伤组织并得到再生植株，在建立的农杆菌转化百合的遗传转化体系中，获得49株再生植株，经PCR检测表明PAP基因已经转移到有抗性愈伤组织再生出百合植株中。

关键词: PAP基因, 麝香百合, 转化, 表达

Abstract: We report an efficient transformation protocol for stable introduction of PAP gene into callus of lily leaf.Calli of Lilium longiflorum leaf were inoculated with Agrobacterium tumefaciens strain LBA4404 containing the binary vector PBI121-1 harboring pokeweed antiviral protein gene,which was isolated from pokeweed leaves.In the selection of antibiotics,the calli of lily were inoculated with Agrobacterium containing PBI121-1 produced small amount of callus on 75mg/L kanamycin and 250mg/L cefotasimine selection plates.Plants were regenerated from resistant calli gained from the slectable medium with MS.An optimum protocol of transformation has been established and 49 regenerative plants from transformations have been obtained.The PAP cDNA integrated into the calli of lily was assayed by PCR,The PCR analyses demonstrated stable integration of PAP gene into the Lilium longiflorum genomes.Expression of the genes was particularly eminent in leaf tissue of primary transformant plants.The establishment of an efficient transformation method may facilitate the improvement of lily in terms of the levels of antiviral protein.

Key words: Pokeweed antiviral protein(PAP), Lilium longif lorum, Transformation, Expression

中图分类号: 

• S644.1