绵羊卵巢腔前卵泡的分离与体外培养

doi:10.3321/j.issn:1000-7091.2004.02.004

华北农学报 ›› 2004, Vol. 19 ›› Issue (2): 13-16. doi: 10.3321/j.issn:1000-7091.2004.02.004

所属专题： 畜牧；

绵羊卵巢腔前卵泡的分离与体外培养

刘海军¹, 薛俊², 徐直¹, 张冬梅¹, 李义海¹

1. 天津市畜牧兽医研究所, 天津 300112;
2. 天津市农业生物技术研究中心, 天津 30092

收稿日期:2003-09-24 出版日期:2004-06-28
作者简介:刘海军(1967-),男,蒙古族,内蒙古赤峰人,博士,副研究员,主要从事动物胚胎生物技术研究工作.
基金资助:
天津市青年科学基金资助项目(973404511)

Isolation and in vitro Culture of Sheep Preantral Follicles

LIU Hai-jun¹, XUE Jun², XU Zhi¹, ZHANG Dong-mei¹, LI Yi-hai¹

1. Tianjin Institute of Animal Science and Veterinary Medicine,Tianjin 300112,China;
2. Tianjin Research Center of Agricultural Biotechnology,Tianjin 300192,China

Received:2003-09-24 Published:2004-06-28

摘要/Abstract

摘要： 分离方法和绵羊年龄对绵羊卵巢腔前卵泡的分离效果有显著影响.从每只羊获得腔前卵泡的总数看,机械分离法(89.5)和酶消化法(103.3)差异不显著,而正常卵泡数和正常卵泡率机械分离法(27.9,31.2%)明显高于酶消化法(19.1,18.5%);采用机械分离法,每只初情期前绵羊获得的腔前卵泡总数和正常卵泡数(125.6,35.1)均高于成年绵羊(60.,16.1),而正常卵泡率(28.1%比26.8%)差异不显著.腔前卵泡在体外培养6 d,培养液中添加20ng/mL的IGF-1,培养结束时卵母细胞直径增幅为3.8 μm,与对照组的29.9 μm差异不显著.

关键词: 绵羊, 腔前卵泡, 分离, 体外培养

Abstract: Isolation method and sheep age had significant effects on the isolation efficiency of sheep preantral follicles.Per sheep mechnical isolation obtained the same total preantral follicles(89.5) with that of enezmy digestion (103.3)(P0.05) per sheep,but normal follicle rate(31.2%) of mechnical isolation was high than that (18.5%) of enezmy digestion (P 0.05).By mechnical isolation, per preputuberty sheep obtained higher total preantral follicles and normal preantral follicles(125.6,35.1)than that of adult sheep (60.4,16.1)(P0.05), but normal follicle rate did not differ(28.1 % vers 26.8% ).Preantral follicles were cultured in vitro for 6 days, no difference was observed in oocyte growth (34.8 μm vs 29.9 μm) between IGF-I (20 ng/mL) treated follicles and controls.

Key words: Sheep, Preantral follicle, Isolation, In vitro culture

中图分类号:

Q813

刘海军, 薛俊, 徐直, 张冬梅, 李义海. 绵羊卵巢腔前卵泡的分离与体外培养[J]. 华北农学报, 2004, 19(2): 13-16. doi: 10.3321/j.issn:1000-7091.2004.02.004.

LIU Hai-jun, XUE Jun, XU Zhi, ZHANG Dong-mei, LI Yi-hai. Isolation and in vitro Culture of Sheep Preantral Follicles[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2004, 19(2): 13-16. doi: 10.3321/j.issn:1000-7091.2004.02.004.

http://www.hbnxb.net/CN/Y2004/V19/I2/13

参考文献

[1] Ireland J J. Control of follicular growth and development[J].J Reprod Fert, 1987,34(增刊):39-54.
[2] Eppig J J,Schroeder A C.Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation and fertilization in vitro[J]. Biol Reprod, 1989,41: 268-276.
[3] Epping J J,OBrien M J.Development in vito of mouse oocytes from primordial follicles[J].Biol Reprod, 1996,54:97-207.
[4] Hiaro Y, Nagai T, Kubo M, et al. In vitro growth and maturation of pig oocytes[J]. J Reprod Fertil, 1994,100:333-339.
[5] Wu J,Emery B R,Carrell D T, et al. In vitro growth,maturation, fertilization, and embryonic development of oocytes from porcine preantral follicles[J]. Biol Reprod.,2001,64:375-381.
[6] Gutierrez C G,Ralph J H, Teller E E, et al. Growth and antrum formation of bovine preantral follicles in long-term culture in vitro[J]. Biol Reprod, 2000,62: 1322-1328.
[7] 周欢敏,张涌.山羊卵巢无腔卵泡卵母细胞的体外生长[J].中国兽医学报,1999,19(3):301-304.
[8] 芮荣,张涌.山羊卵巢腔前卵泡卵母细胞体外发育体外成熟的研究[J].畜牧兽医学报,2000,31(6):481-486.
[9] Cecconi S,Barboni B,Coccia M, et al. In vitro development of sheep preantral follicles[J]. Biol Reprod, 1999,60:594-601.
[10] Newton H,Picton H,Gosden R G. In vitro growth of oocyte-granulosa cell complexes isolated from cryopreserved ovine tissue[J]. J Reprod Fertil, 1999,115:141-150.
[11] Figeiredo J R,Hulshof S C J, Van der Hurk R, et al.Development of a combined new mechanical and enzymatic method for the isolation of intact preantral follicles from fetal, calf and adult bovine ovaries[J]. Theriogenology, 1993,40: 789-799.
[12] McCaffery F H, Leask R, Riley S C, et al.Culture of bovine preantral follicles in a serum-free system:markers for assessment of growth and development[J]. Biol Reprod, 2000,63: 267-273.
[13] Nicosia S V, Evangelista I, Batta S K.Rabbit ovarian follicles. I-Isolation technique and characterization at different stages of development[J]. Biol Reprod, 1975,13:423-447.
[14] Wandji S A,Wood T L,Crawford J, et al. Expression of mouse ovarian insulin-like growth factor system components during follicular development and atresia[J]. Endocrinology, 1998,139: 5205-5214.
[15] Monniaux D,Pisselet C.Control of proliferation and differentiation in ovine granulosa cells by insulin-like growth factor-1 and follicle stimulating hormone in vitro[J].Biol Reprod,1992,46:109-119.
[16] Leeuwenberg B R,Hurst P R, McNatty K P. Expression of IGF-I messenger-RNA in the ovine ovary[J].J Med Endocrinol, 1995,15:251-258.
[17] Yuan W, Lucy M C, Smith M F. Messenger ribonucleic acid for insulin-like growth factors-I and-Ⅱ insulin-like growth factor-binding protein-2 gonadotropin receptors and steroidogenic enzymes in porcine follicles[J ]. Biol Reprod, 1992,55:1045-1054.
[18] Schams D, Berisha B, Kosmann M, et al. Possible role of growth hormone, IGFs, and IGF-bindingproteins in the regulation of ovarian function in large farm animals[J].Domest Anim Endocrinol, 1999,17:279-285.
[19] Gutierrez C G, Armstrong D G, Campbell B K, et al. Insulin-like growth factors(IGF) I production and expression of IGF-Ⅰ and Ⅱ by bovine granulosa and theca cells in vivo and in vitro[J].J Reprod Fertil Abstr Ser, 1996,17 :25 (abstract 66).

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