摘要： 从影响黄瓜转基因体系的外植体类型、农杆菌共浸染时间、是否加入乙酰丁香酮等因素摸索,建立了黄瓜遗传转化体系;将抗除草剂基因bar以农杆菌共浸染法导入到黄瓜父本品种M1子叶中,经历愈伤组织分化、芽诱导和生根等过程获得落地转化株系,通过特异性引物的PCR鉴定,13个株系扩增出bar基因片段。T0自交获得T1转基因植株,经1000倍除草剂巴士达喷施处理,其中84%表现为较高的抗性。
关键词:
黄瓜,
基因bar,
遗传转化,
抗性鉴定
Abstract: The procedure of cucumber trans formation was established by s tudying factors such as types of explants,cotransformation with Agrobacterium tume fac iens and adding acetosyringone or not etc. The antiherbicide gene bar was transferred into the c otyledons of the cucumber father M1 by Agrobacterium tume faciens and the t rans formed cucumber lines were obtained by taking through the proces s of cal lus di fferentiation, shooting and rooting.The PCR detection showed 13 lines showed the existence of the bar gene fragment. T1 plants were got by selfcrossing of T0 plants, 84% of which showed higher resis tance to 1 000 dilution of the herbicide of BASTA.
Key words:
Cucumber,
Bar gene,
Transformation,
Resistance characterization
中图分类号:
金红, 杜胜利, 陈峥, 魏爱民. 抗除草剂转基因黄瓜的获得及T1植株抗性鉴定[J]. 华北农学报, 2003, 18(1): 44-46. doi: 10.3321/j.issn:1000-7091.2003.01.013.
JIN Hong, DU Sheng li, CHEN Zheng, WEI Ai min. The Obtaining of Transgenic Cucumbers Resistant to Herbicide and Resistance Detection of T1 Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 44-46. doi: 10.3321/j.issn:1000-7091.2003.01.013.