摘要： 采用SDS法、尿素法、改良SDS法、氯化苄法和CTAB法对黄瓜的基因组DNA进行提取.结果表明,改良SDS法提取的黄瓜叶片DNA具有典型的天然DNA分子的标准紫外吸收光谱特点,其A₂₆₀/A₂₈₀在1.7～1.9之间,DNA的产量为50 μg/g.用该法提取的黄瓜DNA适合进行RAPD分析.

关键词: 黄瓜, DNA提取方法, RAPD分析

Abstract: The preparat ion of DNA samples with good quality is the basis for researches of plant molecular biology.The DNA samples of cucumber male sterile line YS and maintainer line MC were prepared by SDS, Urea, Improved SDS, Benzyl chloride and CTAB method, respectively. The results showed that DNA samples obtained by improved SDS method possessed the standard ultraviolet absorbance spectrum of the pure natural DNA and the A₂₆₀/ A₂₈₀ was between 1.7- 1.9, the yield of DNA obtained by improved SDS method was 450Lg/g young leaves, and the DNA samples prepared by this procedure were suitable for RAPD analysis.

Key words: Cucumber, DNA ext ract ion, RAPD analysis

中图分类号: 

• S62.2