华北农学报 ›› 2002, Vol. 17 ›› Issue (4): 5-8. doi: 10.3321/j.issn:1000-7091.2002.04.002

所属专题: 甜瓜

• 论文 • 上一篇    下一篇

甜瓜作图亲本间分子标记的多态性

王建设1, 孟淑春1, 宋曙辉1, 陈贵林2, 孙振英2, 李梅2   

  1. 1. 国家蔬菜工程技术研究中心,北京,100089;
    2. 河北农业大学,园艺系,河北,保定,071001
  • 收稿日期:2001-08-15 出版日期:2002-12-28
  • 作者简介:王建设(1966-),男,副研究员,农学博士,主要从事甜瓜分子标记辅助育种研究.

Molecular Marker′s Polymorphism Between Mapping Parents on Cucumis melo L.

WANG Jian-she1, MENG Shu-chun1, SONG Shu-hui1, CHEN Gui-lin2, SUN Zhen-ying2, LI Mei2   

  1. 1. Beijing Vegetable Research Center, Beijing 100089, China;
    2. Hebei Agricultural University, Baoding 071001, China
  • Received:2001-08-15 Published:2002-12-28

摘要: 利用DAMD,ISSR和RAPD 3种不同类型的分子标记研究了作图亲本栽培甜瓜黄旦子与野生甜瓜PI1723间的多态性,结果表明,DAMD和ISSR揭示作图亲本间多态性的效率较高,而RAPD标记揭示作图亲本间多态性的效率较低,说明为了提高甜瓜作图效率,除了选择遗传差异相对较大杂交组合外,关键是选择高效揭示多态性的分子标记类型.

关键词: 甜瓜, 作图亲本, 分子标记, 多态性

Abstract: Polymorphism of mapping parents between cultivated variety Huangdanzi and wild var-iety PI414723 on C.melo L. were analyzed by using 3 different types of molecular markers- RAPD, DAMD and ISSR. The results showed that DAMD and ISSR revealed polymorphism eff-iciently, and RAPD detected polymorphism ineff iciently. This indicated that the key for mapping on C.melo L. is to select the right type of molecular marker which can reveal polymorphism eff-iciently, besides selecting the cross with larger genetic difference.

Key words: Cucumis melo L., Mapping parents, Molecular marker, Polymorphism

中图分类号: 

引用本文

王建设, 孟淑春, 宋曙辉, 陈贵林, 孙振英, 李梅. 甜瓜作图亲本间分子标记的多态性[J]. 华北农学报, 2002, 17(4): 5-8. doi: 10.3321/j.issn:1000-7091.2002.04.002.

WANG Jian-she, MENG Shu-chun, SONG Shu-hui, CHEN Gui-lin, SUN Zhen-ying, LI Mei. Molecular Marker′s Polymorphism Between Mapping Parents on Cucumis melo L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 5-8. doi: 10.3321/j.issn:1000-7091.2002.04.002.

使用本文