摘要： 以4个草莓品种的匍匐茎尖为起始材料,试验以MS为基本培养基附加不同激素草莓茎尖诱导植株再生、增殖、生根的培养基,确立了草莓组培快繁技术方法;又以4个草莓品种的组培苗叶片为外植体,探讨了不同激素配比、基因型对诱导草莓不定芽再生的影响;通过进一步试验,以MS+B有机为基本培养基附加6-BA 2.23 mg/L、IAA 1.72 mg/L,探讨草莓不同基因型及不同外植体对诱导不定芽再生的影响,初步建立了一个有效的、较高频率的芽再生系统,也为基因的转化找到一个较好的受体材料.
关键词:
草莓,
匍匐茎尖,
组培,
快繁,
叶片,
诱导
Abstract: The medium of regeneration plantlet from shoot tip of strawberry creeping stem supplemented with different hormones was worked out.The protocol of tissue culture and rapid propagation has been developed. The influences of hormone condition, genotypes on shoot regeneration from leaf disc of strawberry were examined.By further studying, the effects of genotypes and explants on shoot induction were also examined when basal medium was MS+ B5 supplemented with 2.253 mg/L 6-BA and 1.752 mg/L IAA. An efficient and higher frequence method of direct shoot regeneration has been established. Meanwhile, a suitable receptor material of genetic transformation.
Key words:
Strawberry,
Shoot tip of creeping stem,
Tissue culture,
Rapid propagation,
Leaf disc,
Induction
中图分类号:
孙瑞芬, 李天然, 李堃, 邓香兰, 石慧芹, 张颖力, 贾利敏. 草莓组培快繁及叶片诱导植株再生的研究[J]. 华北农学报, 2002, 17(4): 49-53. doi: 10.3321/j.issn:1000-7091.2002.04.011.
SUN Rui-fen, LI Tian-ran, LI Kun, DENG Xiang-lan, SHI Hui-qin, ZHANG Ying-li, JIA Li-min. Study on Tissue Culture and Regeneration Plantlet from Strawberry Leaf[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 49-53. doi: 10.3321/j.issn:1000-7091.2002.04.011.