草莓生长素响应基因<i>FvIAA17</i>的克隆及表达分析

doi:10.7668/hbnxb.201751591

摘要/Abstract

摘要： Aux/IAA蛋白作为转录抑制因子在植物生长发育过程中有重要的调控作用。为了研究草莓生长素诱导基因FvIAA17在草莓生长发育过程中的功能，以森林草莓为试验材料，克隆草莓FvIAA17基因，并对其序列特征进行生物信息学分析，利用Real-time PCR技术分析了FvIAA17在草莓不同组织及外源激素处理下的表达模式。结果表明，FvIAA17基因开放阅读框为594 bp，编码197个氨基酸；预测其分子质量和等电点分别为21.85 ku和7.56，具有Aux/IAA家族蛋白典型结构域和保守序列；该基因启动子区含有脱落酸、茉莉酸甲酯及胁迫相关的顺时作用元件。进化分析表明，FvIAA17基因与苹果生长素诱导基因MdIAA1亲缘关系最近；亚细胞定位预测分析表明，FvIAA17蛋白定位于周质内。Real-time PCR结果表明，FvIAA17基因表达量受到外源激素IAA、ABA的显著诱导，说明该基因可能参与了生长素、脱落酸调控草莓发育的信号转导过程。本研究为后继研究草莓FvIAA17的功能和作用机制提供参考依据。

关键词: 草莓, 生长素, FvIAA17, 基因克隆

Abstract: Aux/IAA protein plays an important role in regulating plant development as a transcriptional inhibitor. In order to identify the function of FvIAA17 gene in strawberry development, it was cloned by RT-PCR from strawberry. The sequence feature of FvIAA17 gene was analyzed by bioinformatics methods, and its expression patterns were analyzed by Real-time quantitative PCR(qPCR)in different tissues and under different phytohormone treatments. The results showed that FvIAA17 contained a 594 bp open reading frame and encoded 197 amino acid, with a molecular mass of 21.85 ku and an isoelectric point of 7.56. The amino acid sequence alignment showed that FvIAA17 had the typical structure domain and conservative basic sequence of Aux/IAA gene families. Promoter analysis revealed that the sequence of FvIAA17 contained the related response elements to auxin, ABA, MeJA and other stresses. Phylogenetic tree analysis indicated that FvIAA17 was most closely related to MdIAA1 from Malus domestica. FvIAA17 was an auxin response gene and was located in periplasmic space according to subcellular localization. Real-time PCR analysis showed FvIAA17 had specific expression differences in different organs, and it could be induced by exogenous IAA and ABA significantly, indicating that FvIAA17 might play an important role in auxin and abscisic acid signal transduction during strawberry development. This research provided a theoretical basis for further studying the function and molecular regulation mechanism of FvIAA17.

Key words: Strawberry, Auxin, FvIAA17, Gene clone

中图分类号:

Q78
S668.03

苏丽艳. 草莓生长素响应基因FvIAA17的克隆及表达分析[J]. 华北农学报, 2019, 34(4): 46-52. doi: 10.7668/hbnxb.201751591.

SU Liyan. Cloning and Expression Analysis of Auxin Response Gene FvIAA17 in Strawberry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 46-52. doi: 10.7668/hbnxb.201751591.

http://www.hbnxb.net/CN/Y2019/V34/I4/46

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草莓生长素响应基因FvIAA17的克隆及表达分析

Cloning and Expression Analysis of Auxin Response Gene FvIAA17 in Strawberry

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草莓生长素响应基因FvIAA17的克隆及表达分析

Cloning and Expression Analysis of Auxin Response Gene FvIAA17 in Strawberry

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