摘要： 通过大量的接种实验,分析了影响病毒接种效率的各种因素,经ELISA和RT-PCR方法进行检测,确立了较为稳定有效的小麦黄花叶病毒机械传毒体系.采用新鲜病叶或用氯化钙干燥保存的病叶作为接种毒源,在0.2 mol*L-1磷酸缓冲液中研磨后,对低温下(11～13 ℃)培养的小麦幼苗(1～2叶龄)进行叶面接种,并将接种后的小麦低温培养可获得较高的接种效率(12.7%～13.43%).对毒源类型、小麦苗龄和接种部位等其他可能影响接种效率的因素进行了测定.
关键词:
小麦黄花叶病毒,
机械接种,
病毒检测
Abstract: Factors affecting artificial inoculation of WYMV on susceptible wheat variety were analyzed.The result indicated that the highest incidence could be gained by using the dried diseased-leaf or fresh wheat to inoculate wheat cultivated at low temperature.The diseased-leaf should be grounded in phosphate buffer. The effective inoculation method was developed and WYMV in wheat leaves was tested by ELISA and RT-PCR.
Key words:
Wheat yellow mosaic virus,
Mechanical inoculation,
Virus detection
中图分类号:
尚巧霞, 韩成贵, 于嘉林, 杨莉莉, 刘仪. 小麦黄花叶病毒人工侵染体系的研究[J]. 华北农学报, 2002, 17(4): 54-58. doi: 10.3321/j.issn:1000-7091.2002.04.012.
SHANG Qiao-xia, HAN Cheng-gui, YU Jia-lin, YANG Li-li, LIU Yi. Artificial Inoculation of Wheat Yellow Mosaic Virus to Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(4): 54-58. doi: 10.3321/j.issn:1000-7091.2002.04.012.