小麦黄花叶病毒河南驻马店分离物的鉴定与全序列分析

doi:10.7668/hbnxb.2010.02.002

华北农学报 ›› 2010, Vol. 25 ›› Issue (2): 5-11. doi: 10.7668/hbnxb.2010.02.002

所属专题： 小麦；

小麦黄花叶病毒河南驻马店分离物的鉴定与全序列分析

张宗英¹, 徐建美¹, 韩成贵¹, 张振臣², 李大伟³, 于嘉林³

1. 中国农业大学植物病理系, 北京 100193;中国农业大学农学与生物技术国家重点实验室, 北京 100193;
2. 河南省农业科学院植物保护研究所, 河南郑州 450002;
3. 中国农业大学农学与生物技术国家重点实验室, 北京 100193

收稿日期:2009-12-18 出版日期:2010-04-28
通讯作者: 韩成贵(1966-),男,辽宁法库人,教授,博士,主要从事植物病毒的研究。
作者简介:张宗英(1982-),女,山东聊城人,在读博士,主要从事植物病毒学研究.
基金资助:
农业部行业专项(nyzx07-051)

Detective and Complete Sequence Analysis of Wheat Yellow Mosaic Virus from Zhumadian in Henan Province

ZHANG Zong-ying¹, XU Jian-mei¹, HAN Cheng-gui¹, ZHANG Zhen-chen², LI Da-wei³, YU Jia-lin³

1. Department of Plant Pathology, China Agricultural University, Beijing 100193, China;
2. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China;
3. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received:2009-12-18 Published:2010-04-28

摘要/Abstract

摘要： 采集河南省驻马店市13个不同田块小麦样品,利用RT-PCR、ELISA和Western Blotting检测技术进行检测,结果表明,该地有小麦黄花叶病毒的存在与分布。对检测为阳性的一个分离物全序列进行cDNA克隆和测序,与已报道WYMV河南潢川、江苏扬州、四川雅安和日本分离物的全序列进行比对,RNA1核苷酸一致性为97.1%~98.4%;RNA2为95.1%~98.4%;氨基酸一致性分别为94.8%~97.8%和94.2%~98.2%。利用软件MEGA4.1对上述五个RNA1和RNA2的全序列分别进行进化树分析,结果表明,驻马店分离物与潢川亲缘关系最近,而与四川雅安亲缘关系最远。

关键词: 小麦黄花叶病毒, 驻马店分离物, 病毒检测, 基因组全序列

Abstract: A total of 13 wheat samples from different fields in Zhumadian of Henan Province were investigated,using RT-PCR,ELISA and Western Blotting,the results showed that WYMV existed there.Complete RNA genomic cDNA of one positive isolate(WYMV-ZMD)was cloned and sequenced,and was compared with those of isolates from Huangchuan,Yangzhou,Ya'an and Japan.Sequence comparisons showed that WYMV-ZMD shared 97.1%-98.4% nucleotide sequence identity for RNA1 with the reported isolates,and 94.2%-98.2% identity for RNA2.At the amino acid level,WYMV-ZMD had 94.8%-97.8% identity for RNA1,and 94.2%-98.2% identity for RNA2,respectively.Further phylogenetic analysis of five different isolates based on RNA1 and RNA2 by MEGA 4.1 indicated that WYMV-ZMD was more closely related to WYMV-HC,and had a more distant relationship with Ya'an isolate.

Key words: Wheat yellow mosaic virus, Zhumadian isolate, Detection, Complete sequence

中图分类号:

张宗英, 徐建美, 韩成贵, 张振臣, 李大伟, 于嘉林. 小麦黄花叶病毒河南驻马店分离物的鉴定与全序列分析[J]. 华北农学报, 2010, 25(2): 5-11. doi: 10.7668/hbnxb.2010.02.002.

ZHANG Zong-ying, XU Jian-mei, HAN Cheng-gui, ZHANG Zhen-chen, LI Da-wei, YU Jia-lin. Detective and Complete Sequence Analysis of Wheat Yellow Mosaic Virus from Zhumadian in Henan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 5-11. doi: 10.7668/hbnxb.2010.02.002.

http://www.hbnxb.net/CN/Y2010/V25/I2/5

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