阔褶水蛙皮肤组织抗菌肽latouchiin-1和latouchiin-2的基因克隆和序列分析

doi:10.7668/hbnxb.2010.02.003

华北农学报 ›› 2010, Vol. 25 ›› Issue (2): 12-17. doi: 10.7668/hbnxb.2010.02.003

所属专题： 生物技术；

阔褶水蛙皮肤组织抗菌肽latouchiin-1和latouchiin-2的基因克隆和序列分析

王辉¹, 郧海丽², 赵军锋³, 王多⁴, 冉然⁴, 胡玉红⁵

1. 河北师范大学实验中心, 河北石家庄 050016;河北师范大学生命科学学院, 河北石家庄 050016;
2. 石家庄学院化工学院, 河北石家庄 050035;
3. 河北师范大学汇华学院, 河北石家庄 050091;
4. 河北师范大学生命科学学院, 河北石家庄 050016;
5. 河北师范大学实验中心, 河北石家庄 050016

收稿日期:2010-01-26 出版日期:2010-04-28
通讯作者: 胡玉红(1980-),女,河北石家庄人,实验师,硕士,主要从事功能性蛋白分离及开发应用研究。
作者简介:王辉(1981-),男,河北石家庄人,讲师,博士,主要从事两栖类功能性多肽研究.
基金资助:
河北师范大学青年基金(L2007Q32)

Molecule Cloning and Sequence Analysis of the cDNA Encoding Antimicrobial Peptides latouchiin-1 and latouchiin-2 from Hylarana latouchii Skin Tissue

WANG Hui¹, YUN Hai-li², ZHAO Jun-feng³, WANG Duo⁴, RAN Ran⁴, HU Yu-hong⁵

1. Experimental Center, Hebei Normal University, Shijiazhuang 050016, China;
2. College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, China;
3. College of Chemical Engineering, Shijiazhuang University, Shijiazhuang 050035, China;
4. College of Huihua, Hebei Normal University, Shijiazhuang 050091, China

Received:2010-01-26 Published:2010-04-28

摘要/Abstract

摘要： 为了从生物体中寻找新颖的抗菌肽类药物分子,以阔褶水蛙(Hylarana latouchii)为研究材料,运用3′RACE技术成功从其皮肤组织中筛选出2个抗菌肽前体cDNA编码序列,经blast生物信息学分析,确定其为首次发现,推导出的这2条新颖的抗菌肽序列,命名为latouchiin-1和latouchiin-2。

关键词: 阔褶水蛙, 抗菌肽, 分子克隆, 皮肤组织

Abstract: In this study,we used Hylarana latouchii as the material to find novel antimicrobial peptides from native organism.Two different cDNAs encoding two novel antimicrobial peptide precursors were cloned by 3' RACE technique from skin of H.latouchii.There was no homologous sequence with these two cDNA sequences by blasting analyse.These two novel antimicrobial peptide precursors were named as latouchiin-1 and latouchiin-2.

Key words: Hylarana latouchii, Antimicrobial pep tides, Molecule cloning, Skin tissue

中图分类号:

Q785

王辉, 郧海丽, 赵军锋, 王多, 冉然, 胡玉红. 阔褶水蛙皮肤组织抗菌肽latouchiin-1和latouchiin-2的基因克隆和序列分析[J]. 华北农学报, 2010, 25(2): 12-17. doi: 10.7668/hbnxb.2010.02.003.

WANG Hui, YUN Hai-li, ZHAO Jun-feng, WANG Duo, RAN Ran, HU Yu-hong. Molecule Cloning and Sequence Analysis of the cDNA Encoding Antimicrobial Peptides latouchiin-1 and latouchiin-2 from Hylarana latouchii Skin Tissue[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(2): 12-17. doi: 10.7668/hbnxb.2010.02.003.

http://www.hbnxb.net/CN/Y2010/V25/I2/12

参考文献

[1] Conlon J M,Al-Ghaferi N,Abraham B,et al.Strategies for transformation of naturally-occurring amphibian antimicrobial peptides into therapeutically valuable anti-infective agents[J].Methods,2007,42(2):349-357.
[2] 王辉,张秀清,胡玉红,等.阔褶水蛙皮肤组织cDNA文库的构建及抗菌肽brevinin-2LTs的cDNA克隆和序列分析[J].华北农学报,2009,24(增刊):9-14.
[3] Park Y,Lee D G,Jang S H,et al.A Leu-Lys-rich antimicrobial peptide:activity and mechanism[J].Biochim Biophys Acta,2003,1645(2):172-182.
[4] Skerlavaj B,Benincasa M,Risso A,et al.SMAP-29:A potent antibacterial and antifungal peptide from sheep leucocytes[J].FEBS Lett,1999,463(1-2):58-62.
[5] Wang H X,Ng T B.Isolation and characterization of an antifungal peptide with antiproliferative activity from seeds of Phaseolus vulgaris cv.′Spotted Bean′[J].Appl Microbiol Biotechnol,2007,74(1):125-130.
[6] Wu S,Jia S,Sun D,et al.Purification and characterization of two novel antimicrobial peptides Subpeptin JM4-A and subpeptin JM4-B produced by Bacillus subtilis JM4[J].Curr Microbiol,2005,51(5):292-296.
[7] Zhou J,McClean S,Thompson A,et al.Purification and characterization of novel antimicrobial peptides from the skin secretion of Hylarana guentheri[J].Peptides,2006,27(12):3077-3084.
[8] Chen T,Li L,Zhou M,et al.Amphibian skin peptides and their corresponding cDNAs from single lyophilized secretion samples:identification of novel brevinins from three species of Chinese frogs[J].Peptides,2006,27(1):42-48.
[9] Suzuki H,Iwamuro S,Ohnuma A,et al.Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii[J].Peptides,2007,28(3):505-514.
[10] Conlon J M,Kolodziejek J,Nowotny N.Antimicrobial peptides from ranid frogs:taxonomic and phylogenetic markers and a potential source of new therapeutic agents[J].Biochim Biophys Acta,2004,1696(1):1-14.
[11] Conlon J M,Sonnevend A,Patel M,et al.A family of brevinin-2 peptides with potent activity against Pseudomonas aeruginosa from the skin of the Hokkaido frog,Rana pirica[J].Regul Pept,2004,118(3):135-141.
[12] Park J M,Jung J E,Lee B J.Antimicrobial peptides from the skin of a Korean frog,Rana rugosa[J].Biochem Biophys Res Commun,1994,205(1):948-954.
[13] Conlon J M,Al-Kharrge R,Ahmed E,et al.Effect of aminoisobutyric acid(Aib)substitutions on the antimicrobial and cytolytic activities of the frog skin peptide,temporin-1DRa[J].Peptides,2007,28(10):2075-2080.
[14] 赵炜,郑佐华,毛裕民.全长cDNA的获得-RACE及其他方法进展[J].生命科学,1999,11(2):92-96.
[15] Bevins C L,Zasloff M.Peptides from frog skin[J].Annu Rev Biochem,1990,59:395-414.
[16] 谢锋,刘惠宁,Stuart N S,等.中国两栖动物保护需求总述[J].中国科学:C辑,2006,36(6):570-581.
[17] 赖仞,赵宇,刘衡,等.两栖类动物皮肤活性物质的利用兼论中国两栖类资源开发的策略[J].动物学研究,2002,23(1):65-70.

选择文件类型/文献管理软件名称

选择包含的内容

阔褶水蛙皮肤组织抗菌肽latouchiin-1和latouchiin-2的基因克隆和序列分析

Molecule Cloning and Sequence Analysis of the cDNA Encoding Antimicrobial Peptides latouchiin-1 and latouchiin-2 from Hylarana latouchii Skin Tissue

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	谢昆, 李密杰, 蒋成砚, 鲁海菊, 孔琼, 何超, 沈登荣. *抗菌肽Omiganan的克隆、表达和抑菌活性*[J]. 华北农学报, 2021, 36(2): 62-67.
[2]	谢昆, 王靖, 王丽仙, 朱灵明, 尹建华, 叶青霞, 孙艳. 家蚕抗菌肽基因的克隆及重组蛋白的自诱导表达[J]. 华北农学报, 2018, 33(1): 33-38.
[3]	陈晓武, 施志仪, 李倩, 江山. 牙鲆肝脏抗菌肽-2基因序列和表达分析[J]. 华北农学报, 2012, 27(S1): 12-17.
[4]	胡瑞萍, 张铎, 张丽萍, 张根伟, 张蕾, 赵宝华. 枯草芽孢杆菌BSD-2一种抗菌肽的分离纯化与鉴定[J]. 华北农学报, 2011, 26(6): 201-206.
[5]	杨凯, 韩伟, 温莹, 刘丹丹, 薛春蕾, 逯晓萍. 转Bt和抗菌肽融合基因油菜植株的获得与鉴定研究[J]. 华北农学报, 2011, 26(2): 49-54.
[6]	王辉, 张秀清, 胡玉红, 孙君社. 虎纹蛙皮肤组织cDNA文库的构建及抗菌肽基因tigerinin-HRs的克隆[J]. 华北农学报, 2010, 25(1): 61-67.
[7]	王辉,张秀清,胡玉红,刘敬兰,孙君社. 阔褶水蛙皮肤组织cDNA文库的构建及抗菌肽brevinin-2LTs的cDNA克隆和序列分析[J]. 华北农学报, 2009, 24(S1): 9-14.
[8]	索相敏, 陆秀君, 董建臻, 李静, 李瑞军, 刘顺. 2种昆虫高活性抗菌肽制备方法的优化[J]. 华北农学报, 2007, 22(3): 119-122.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

阔褶水蛙皮肤组织抗菌肽latouchiin-1和latouchiin-2的基因克隆和序列分析

Molecule Cloning and Sequence Analysis of the cDNA Encoding Antimicrobial Peptides latouchiin-1 and latouchiin-2 from Hylarana latouchii Skin Tissue

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价