摘要： 用4×4完全双列杂交研究了甘蓝型油菜小孢子培养胚状体发生能力的遗传规律。结果表明：多数F1的胚产量与具高胚发生能力的亲本相近，部分F₁的胚产量与双亲中亲值接近。胚状体发生能力主要由基因的加性效应控制。高胚发生能力由显性核基因控制。广义和狭义遗传力分别为97.2％和81.1％。由Lisandra（高胚发生能）×Kamikita（低胚发生能）的F₂群体胚发生数的分离结果得出小孢子胚状体发生能力由具加性效应特点的两个基因位点控制。

关键词: 甘蓝型油菜(Brassica napus L.), 小孢子培养, 胚状体发生能力, 遗传

Abstract: Inheritance of microspore embryogenic ability in B.napus was examined by 4×4 diallel crosses using 2 high responsive cultivars (`Topas' and `Lisandra') and 2 low and no responsive ones (`Kizakino' and `Kamikita').Most of F₁ hybrids showed that embryo yields similar to the high responsive parent and some showed embryo yields similar to the mid parent value.Diallel analyses showed that both additive and dominant effects were significant at 1% level in the genetic control of microspore embryogenic ability.Additive gene effects were predominant.High embryogenic ability was controlled by dominant nuclear genes.The broad-and narrow-sense heritabilities were 97.2% and 81.1%,respectively.From the results of segregation of embryo yields in F₂ population of`Lisandra'×`Kamikita',it is concluded that the microspore embryogenic ability was controlled by two loci with additive effects.

Key words: Brassica napus L., Microspore culture, Embryogenic ability, Inheritance, Diallel analysis

中图分类号: 

• S34.3