PbMYB转录因子在鸭梨果心褐变中的表达特征与自激活功能解析

doi:10.7668/hbnxb.20196791

摘要/Abstract

摘要：

为探究不同成熟度鸭梨果心褐变调控的分子机制,以不同采收期鸭梨为材料,采用实时荧光定量PCR技术分析7个PbMYB转录因子基因在贮藏期的表达模式,克隆获得PbMYB1~PbMYB7完整的编码序列,利用酵母单杂交系统检测其转录自激活功能。结果发现,7个编码基因(PbMYBs)表达呈现明显的时序与采收期特异性,与贮藏0 d相比,早采鸭梨贮藏至120 d,果心的PbMYB1、PbMYB4、PbMYB5、PbMYB6、PbMYB7基因显著上调且表达量最高;晚采鸭梨贮藏至210 d时,7个基因呈现上调表达,果心的PbMYB2、PbMYB3成为优势基因;中采鸭梨果心7个基因的表达相对平稳,贮藏至240 d,褐变指数最低(0.59),显著低于早采和晚采鸭梨,与7个转录因子的基因相对表达量密切相关。根据鸭梨MYB转录因子CDS序列分别克隆出PbMYB1至PbMYB7转录因子基因的全长,大小依次为2 937,960,5 088,2 052,891,972,2 133 bp,经过测序比对确定克隆成功。对7个转录因子进行自激活功能验证,3个获得成功,其中转录因子PbMYB2与PbMYB6具有自激活活性;转录因子PbMYB5无自激活活性。PbMYB2与PbMYB6可能作为激活子在鸭梨果心褐变过程中发挥作用,为解析鸭梨果心褐变分子机制及创制抗褐变新种质提供了关键靶点。

关键词: 鸭梨果心, PbMYB转录因子, 成熟度, 表达模式, 转录自激活

Abstract:

To investigate the molecular mechanism underlying core browning regulation in Yali pears at different maturity stages,qRT-PCR was used to analyze the expression patterns of seven PbMYB transcription factor genes during storage in Yali pears.The complete coding sequences of PbMYB1—PbMYB7 were cloned and their transcriptional activation activities were evaluated using a yeast one-hybrid system.PbMYBs exhibited distinct temporal and maturity-specific expression profiles.Compared with those at 0 d,in early-harvested pears,PbMYB1,PbMYB4,PbMYB5,PbMYB6 and PbMYB7 were significantly up-regulated in the core at 120 d,reaching peak expression levels.In late-harvested pears,seven genes were upregulated,PbMYB2 and PbMYB3 became the predominant genes at 210 d.Mid-harvested pears showed relatively stable expression of all seven genes.At 240 d,the browning index of mid-harvested pears was the lowest(0.59),significantly lower than that of early- and late-harvested pears,and was closely correlated with the relative expression levels of the seven transcription factor genes.The full-length CDS of PbMYB1—PbMYB7 were successfully cloned,yielding fragments of 2 937,960,5 088,2 052,891,972,and 2 133 bp,respectively,as confirmed by sequencing.Self-activation assays of the seven transcription factors showed that three were successfully evaluated;PbMYB2 and PbMYB6 exhibited self-activation activity,whereas PbMYB5 did not.The self-activation of PbMYB2 and PbMYB6 suggests their potential roles as transcriptional activators,providing critical target genes for dissecting the molecular mechanisms of core browning and developing novel anti-browning germplasm.

Key words: Yali pear core, PbMYB transcription factor, Maturation, Expression pattern, Transcriptional autoactivation

中图分类号:

S661.2

李玲, 李刚, 梁丽雅. PbMYB转录因子在鸭梨果心褐变中的表达特征与自激活功能解析[J]. 华北农学报, 2026, 41(5): 41-50. doi: 10.7668/hbnxb.20196791.

LI Ling, LI Gang, LIANG Liya. Expression Profiles and Autoactivation Function Analysis of PbMYB Transcription Factors in Core Browning of Yali Pear[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(5): 41-50. doi: 10.7668/hbnxb.20196791.

http://www.hbnxb.net/CN/Y2026/V41/I5/41

图/表 13

表1 qRT-PCR引物序列

Tab.1 Primer sequence of qRT-PCR

基因名称 Gene name	引物序列(5'—3') Primer sequences (5'—3')	产物长度/bp Product length
PbMYB1	F:AAGGCTGCTGGCATTGATACTAGG	141
	R:GTTAGGCTGCTCCACTGGTCTATC
PbMYB2	F:AGCAACCAGAACGGCGAGATG	104
	R:GCCATGTAGTTCCTCACCTCCTTC
PbMYB3	F:GGATCGTCTCGGTCTGGTGATAAG	93
	R:CCTACTGTTCCTGCCATACCTTCC
PbMYB4	F:AAGCAGACAGATTCATCCGAGGAC	116
	R:TGTGTAACCTCACCAAGAGTCCAAG
PbMYB5	F:AGGTTGAAGCAGAAGATAGGAGGAG	110
	R:GCCGTTAGATGTTGGACAGTGTTG
PbMYB6	F:CCTGTCCATGAATTTGACCAATCCC	84
	R:TTCCCTGTATCCGCATCCATCTTG
PbMYB7	F:TGACTGGCAAGAAGGAAGGACTAAG	171
	R:CGTCAATTTCATCGGACTTCAAGGG

表2 全长引物序列

Tab.2 Full-length primer sequences

基因名称 Gene name	引物序列(5'—3') Primer sequences(5'—3')	产物长度/bp Product length
PbMYB1	F:ATGAGGATTATGATCAAAGGAGGC	2 937
	R:TTAGATTTGTGGTGCTGCATTTTCAG
PbMYB2	F:ATGGCTTCCACAAAGAAAGTGG	960
	R:CTACTCGATTCTGCTAATCCCG
PbMYB3	F:ATGCCGCCAGAACCATTGCC	5 088
	R:CTACCTGCCTATATCCCCTTTG
PbMYB4	F:ATGGAAACAGTGGTCGGTGTTG	2 052
	R:CTACAAGAAGCCCGATCTCATT
PbMYB5	F:ATGAGAGCTGGTATGTCCACTA	891
	R:CTACATGAAAAAATCACTGTCAAACC
PbMYB6	F:ATGGTATCCAAAAACCCGAACC	972
	R:TTAAGCAACTTTCTCCTGTTGATCG
PbMYB7	F:ATGTCGGATTGGGACGATGTTT	2 133
	R:TTAGTCGGACCATGTAAAATCTTCTT

表3 用于构建PbMYB2/5/6自激活载体的引物

Tab.3 Primers for constructing PbMYB2/5/6 self-activating vectors

引物名称 Primer name	引物序列(5'—3') Primer sequences(5'—3')
pGBKT7-PbMYB2-F	tcagaggaggacctgcatatgATGGCTTCCACAAAGAAAGTGG
pGBKT7-PbMYB2-R	ccgctgcaggtcgacggatccCTACTCGATTCTGCTAATCCCG
pGBKT7-PbMYB5-F	tcagaggaggacctgcatatgATGAGAGCTGGTATGTCCACTA
pGBKT7-PbMYB5-R	ccgctgcaggtcgacggatccCTACATGAAAAAATCACTGTCAAACC
pGBKT7-PbMYB6-F	tcagaggaggacctgcatatgATGGTATCCAAAAACCCGAACC
pGBKT7-PbMYB6-R	ccgctgcaggtcgacggatccTTAAGCAACTTTCTCCTGTTGATCG

图1 鸭梨果心总RNA琼脂糖凝胶电泳图 1—9.早采贮藏0,30,60,90,120,150,180,210,240 d的鸭梨果心;10—18.中采贮藏0,30,60,90,120,150,180,210,240 d的鸭梨果心;19—27.晚采贮藏0,30,60,90,120,150,180,210,240 d的鸭梨果心。

Fig.1 Agarose gel electrophoresis of total RNA in the core of Yali pear 1—9.Fruit core of early-harvested Yali pears stored for 0, 30, 60, 90, 120, 150, 180, 210, 240 d;10—18.Fruit core of mid-harvested Yali pears stored for 0, 30, 60, 90, 120, 150, 180, 210, 240 d;19—27.Fruit core of late-harvested Yali pears stored for 0, 30, 60, 90, 120, 150, 180, 210, 240 d.

表4 鸭梨果心组织总RNA浓度与质量

Tab.4 Total RNA concentration and quality of Yali pear core tissue

泳道 Lanes	贮藏时期/d Storage period		浓度/(ng/μL) Concentration	A_260/280	A_260/230
1	早采	0	1 050.0	1.85	1.41
2		30	425.1	2.03	1.66
3		60	244.1	1.87	1.37
4		90	326.7	1.96	1.55
5		120	312.7	1.89	1.99
6		150	635.4	1.86	1.89
7		180	654.9	1.86	2.06
8		210	652.4	1.98	1.96
9		240	265.6	1.92	1.68
10	中采	0	467.0	1.83	1.28
11		30	369.7	2.01	1.80
12		60	400.3	1.93	1.44
13		90	203.8	2.00	1.55
14		120	250.6	1.87	1.87
15		150	365.4	2.06	2.12
16		180	634.8	1.97	2.16
17		210	458.9	1.86	2.10
18		240	354.7	1.91	1.26
19	晚采	0	361.5	1.93	1.41
20		30	416.8	2.05	2.13
21		60	430.5	1.88	1.94
22		90	410.8	2.00	1.80
23		120	564.2	1.92	1.82
24		150	456.2	1.86	1.96
25		180	395.6	2.01	2.06
26		210	548.3	1.97	2.04
27		240	612.6	2.00	1.83

图2 鸭梨果心PbMYB基因相对表达量分析图中柱上不同小写字母表示差异显著(P<0.05);相同字母表示差异不显著。图3同。

Fig.2 Analysis of the relative expression of PbMYB genes in the core of Yali pear Different lowercase letters above bars indicate significant differences at P<0.05;while the same letters indicate no significant difference.The same as Fig.3.

图3 不同成熟度鸭梨贮藏期间果心褐变指数

Fig.3 Core browning index of Yali pear with different maturity during storage

图4 鸭梨果心褐变指数与PbMYB基因相对表达量的相关性分析 A.早采鸭梨果心褐变指数与PbMYB基因相对表达量的相关性分析;B.中采鸭梨果心褐变指数与PbMYB基因相对表达量的相关性分析;C.晚采鸭梨果心褐变指数与PbMYB基因相对表达量的相关性分析。

Fig.4 Correlation between core browning index and relative expression levels of PbMYB genes in Yali pear A.Correlation analysis between core index and relative expression of PbMYB gene in early harvest Yali pear;B.Correlation analysis between the core index and the relative expression of PbMYB gene in middle harvest Yali pear;C.Correlation analysis between the core index and the relative expression of PbMYB gene in late harvest Yali pear.

图5 PbMYB基因全长PCR扩增电泳图 M₁.DNA Marker(DL5000);M₂.DNA Marker(DL2000); 1—7 为不同PbMYB 基因的扩增产物。点样按片段大小相近原则分组上样,各泳道对应基因:1.PbMYB1基因,2.PbMYB3基因,3.PbMYB4基因,4.PbMYB7基因,5.PbMYB2基因,6.PbMYB5基因,7.PbMYB6基因。图6同。

Fig.5 Electrophoretogram of PbMYB gene by full-length PCR M₁.DNA Marker(DL5000);M₂.DNA Marker(DL2000);1—7 correspond to the amplification products of different PbMYB genes.Samples were grouped by fragment size rather than loaded sequentially.The gene assignment for each lane:1.PbMYB1 gene,2.PbMYB3 gene,3.PbMYB4 gene,4.PbMYB7 gene,5.PbMYB2 gene,6. PbMYB5 gene,7.PbMYB6 gene.The same as Fig.6.

图6 PbMYB菌落PCR阳性克隆电泳图

Fig.6 Electrophoresis of PbMYB colony PCR positive clones

表5 质粒浓度

Tab.5 Plasmid concentration

基因名称 Gene name	浓度/(ng/μL) Concentration	A_260/280	A_260/230
PbMYB1	198.3	1.96	2.13
PbMYB2	313.5	1.97	2.01
PbMYB3	234.1	1.97	2.17
PbMYB4	189.3	1.93	1.91
PbMYB5	356.4	1.94	2.03
PbMYB6	364.2	1.99	2.01
PbMYB7	206.3	1.97	1.98

图7 二缺培养基上的生长情况 A-1.空载体;B-1.pGBKT7-PbMYB2;C-1.pGBKT7-PbMYB5;D-1.pGBKT7-PbMYB6。右图平板为左图平板的重复。30 ℃恒温培养箱倒置培养2~3 d,直至长出清晰的单菌落,观察自激活情况。图8同。

Fig.7 Growth on two deficiency culture A-1.Empty vector;B-1.pGBKT7-PbMYB2;C-1.pGBKT7-PbMYB5;D-1.pGBKT7-PbMYB6.Right panel is a replicate of the left panel.The transformed yeast cells were grown at 30 ℃ for 2—3 days until distinct colonies appeared,followed by self-activation analysis.The same as Fig.8.

图8 四缺培养基上的生长情况

Fig.8 Growth on four deficiency culture

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