甘蔗E3泛素连接酶基因<i>PRT1</i>的生物信息学及表达模式分析

doi:10.7668/hbnxb.20193378

华北农学报 ›› 2023, Vol. 38 ›› Issue (1): 168-177. doi: 10.7668/hbnxb.20193378

所属专题： 生物技术；

• 资源环境·植物保护 • 上一篇下一篇

甘蔗E3泛素连接酶基因PRT1的生物信息学及表达模式分析

李姗姗¹, 黄梦婷², 青雨虹¹, 许静¹, 黄君梅¹, 凌辉¹, 阙友雄³, 黄宁¹^,

¹ 玉林师范学院生物与制药学院,广西高校亚热带生物资源保护与利用重点实验室,广西玉林 537000
² 太原师范学院生物系,山西晋中 030619
³ 福建农林大学国家甘蔗工程技术研究中心,福建福州 350002

收稿日期:2022-05-18 出版日期:2023-02-28
通讯作者:
黄宁(1989—),女,山东枣庄人,讲师,博士,主要从事植物分子生物学与分子育种研究。
作者简介:
李姗姗(1990—),女,广西陆川人,实验师,硕士,主要从事植物分子生物学与基因克隆研究。
李姗姗、黄梦婷为同等贡献作者。
基金资助:
国家自然科学基金项目(31901592); 国家自然科学基金项目(32160435); 广西自然科学基金项目(2021JJB130026); 广西自然科学基金项目; 玉林师范学院高层次人才启动项目(G2022ZK15)

Bioinformatics and Expression Pattern Analysis of E3 Ubiquitin Ligase Gene PRT1 in Sugarcane

LI Shanshan¹, HUANG Mengting², QING Yuhong¹, XU Jing¹, HUANG Junmei¹, LING Hui¹, QUE Youxiong³, HUANG Ning¹^,

¹ College of Biology and Pharmay,Yulin Normal University,Key Laboratory for Conservation and Utilization of Subtropical Bio-Resources,Education Department of Guangxi Zhuang Autonomous Region,Yulin 537000,China
² Department of Biology,Taiyuan Normal University,Jinzhong 030619,China
³ National Engineering Research Center for Sugarcane,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Received:2022-05-18 Published:2023-02-28
Supported by:
2020GXNSFDA297016

摘要/Abstract

摘要：

为探究甘蔗 PROTEOLYSIS 1(PRT1)基因与黑穗病菌的互作关系,以甘蔗割手密种和栽培品种为研究对象,通过RT-PCR技术对ScPRT1 (GenBank 登录号:MT747433)基因进行克隆,生物信息学分析、定量PCR、亚细胞定位和基因共表达网络分析。生物信息学分析表明,该基因的cDNA全长为1 621 bp,包含一个长度为1 260 bp,编码419个氨基酸的完整开放读码框;其编码蛋白的分子量为46.56 ku,为酸性、不稳定的亲水蛋白,无信号肽,具有核定位信号;该蛋白包含2个RING结构域和1个ZZ结构域;ScPRT1蛋白的高级结构元件多为无规则卷曲。定量PCR 结果显示,ScPRT1在皮、叶片、芽中的表达量相差不大,在蔗髓中表达量最高。同时该基因的表达受脱落酸胁迫后显著上调;受黑穗病菌胁迫后,在甘蔗抗黑穗病品种中上调表达,在感黑穗病品种中下调表达,都达到了显著水平。亚细胞定位结果揭示,ScPRT1定位于细胞核。寄主甘蔗和病原黑穗病菌的基因共表达网络中,与甘蔗ScPRT1共表达的黑穗病菌基因中,有3个为N端具有芳香族氨基酸残基的黑穗病菌效应蛋白,这暗示它们之间存在直接的相互作用。以上结果表明,ScPRT1在甘蔗激素信号传导以及响应黑穗病菌侵染过程中发挥重要作用。

关键词: 甘蔗, ScPRT1, 泛素连接酶, 生物信息学, 表达模式

Abstract:

In order to explore the interaction between ScPRT1 and Sporisorium scitamineum genes in sugarcane,this study used Saccharum spontaneum and Saccharum as research objects.The ScPRT1(GenBank Accession Number:MT747433)gene was cloned by RT-PCR and was analyzed by bioinformatics,quantitative Real-time PCR and subcellular localization.Bioinformatics analysis showed that the full-length cDNA of the ScPRT1 gene was 1 621 bp,including a complete open reading frame with 1 260 bp in length and encodes 419 amino acids.The ScPRT1 protein with two RING domains and one ZZ domain,was 46.56 ku in molecular weight and was an acidic and unstable hydrophilic protein with nuclear localization signal and no signal peptide.The higher structural elements of protein were mainly random coil.The results of qRT-PCR analysis showed that ScPRT1 was higher expressed in stem pith than in bud,epidermis and leaf.And ScPRT1 was significantly up-regulated under abscisic acid(ABA)stress and in the smut-resistant variety but significantly down-regulated in the smut-susceptible variety under Sporisorium scitamineum stress.The result of subcellular localization showed that ScPRT1 was localized in the nucleus.In the co-expression network between ScPRT1 and Sporisorium scitamineum genes,three genes that encoded effectors with aromatic amino acid residues at the N-terminal were found co-expressing with ScPRT1,and their proteins may interact directly.It is speculated that ScPRT1 plays an important role in sugarcane hormone signal transduction and response to smut infection.

Key words: Sugarcane, ScPRT1, Ubiquitin ligase, Bioinformatics, Expression pattern

李姗姗, 黄梦婷, 青雨虹, 许静, 黄君梅, 凌辉, 阙友雄, 黄宁. 甘蔗E3泛素连接酶基因PRT1的生物信息学及表达模式分析[J]. 华北农学报, 2023, 38(1): 168-177. doi: 10.7668/hbnxb.20193378.

LI Shanshan, HUANG Mengting, QING Yuhong, XU Jing, HUANG Junmei, LING Hui, QUE Youxiong, HUANG Ning. Bioinformatics and Expression Pattern Analysis of E3 Ubiquitin Ligase Gene PRT1 in Sugarcane[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 168-177. doi: 10.7668/hbnxb.20193378.

http://www.hbnxb.net/CN/Y2023/V38/I1/168

图/表 7

表1 本研究中所用引物

Tab.1 Primers used in this study

引物Primer	正义链/反义链引物序列(5'—3') Sense primer/Anti-sense primer (5'—3')
ScPRT1	GTGGCCAGACGAATCGAGG/TTTCGTTCACGCAGTTCATCC
YFP-ScPRT1	CGAACGATACTCGAGGTCGACATGACAAGTTTGTACAAAAAAGTTGGGT/GCTCACCATACTAGTGGATCCGAGTGCCTGATCCTCCTCGT
q-ScPRT1	TCATGCCCTCTCTGTACGGA/AATGCACCAGGAGCTACCAC
SARMp1	TGGACTTGGTCAGTTGGAAACA/TGTTCCTGAAGCCTATGTTGCT
ACAD	CGTGGCATGGATCTGATGGT/AGCCTGCTCCAGTTCAATCC
CUL	TGCTGAATGTGTTGAGCAGC/TTGTCGCGCTCCAAGTAGTC
CAC	ACAACGTCAGGCAAAGCAAA/AGATCAACTCCACCTCTGCG

图1 甘蔗割手密种和栽培品种PRT1基因的鉴定和分析 A.甘蔗割手密种和栽培品种PRT1s保守结构域分析;B.甘蔗割手密种PRT1s染色体分布;C.甘蔗割手密种PRT1s顺式元件预测;D.甘蔗割手密种和栽培品种PRT1s氨基酸序列比对。

Fig.1 Identification and analysis of PRT1 gene in Saccharum spontaneum and Saccharum hybrid cultivar A.Analysis of conserved domain of PRT1s from S.spontaneum and S. hybrid cultivar;B.Chromosomal location of PRT1s in S.spontaneum;C.Prediction of cis-elements of PRT1s in S.spontaneum;D.Sequence alignment of PRT1s from S.spontaneum and S. hybrid cultivar.

图2 甘蔗栽培品种PRT1序列的生物信息学分析 A.甘蔗栽培种PRT1基因PCR产物的琼脂糖电泳结果:M.Marker 1 kb plus DNA Marker(全式金,北京,中国);B.甘蔗栽培品种PRT1保守结构域;C.甘蔗栽培品种PRT1核酸序列及其编码的氨基酸序列;D.甘蔗栽培品种PRT1三级结构。

Fig.2 Bioinformatics analysis of sugarcane ScPRT1 A.The agarose electrophoretic result of PCR product of S.hybrid cultivar PRT1 gene:M.Marker 1 kb plus DNA Marker(Transgen,Beijing,China);B.Conserved domain prediction of PRT1 in S.hybrid cultivar;C.Nucleotide sequence of the PRT1 gene and its encoded amino acid sequence in S. hybrid cultivar;D.Tertiary structure of PRT1 in S. hybrid cultivar.

图3 ScPRT1基因在不同组织中的表达图中不同小写字母表示差异显著(P<0.05)。图4同。

Fig.3 Expression of ScPRT1 gene in different tissues Different lowercase letters indicate significant difference(P<0.05).The same as Fig.4.

图4 ScPRT1基因在不同胁迫下的表达 A.脱落酸、茉莉酸甲酯和水杨酸胁迫;B.黑穗病菌胁迫。

Fig.4 Expression of ScPRT1 gene under different stresses A.Salicylic acid,Methyl jasmonate and abscisic acid stress;B.Sporisorium scitamineum stress.

图5 ScPRT1在本氏烟叶片表皮细胞中的亚细胞定位图片包括荧光场、明场以及两场叠加。35S∷YFP.空载pCAMBIA2300-YFP在本氏烟叶片上的瞬时表达图片。35S∷ScPRT1∷YFP.载体pCAMBIA 2300-ScPRT1-YFP在本氏烟叶片上的瞬时表达图片。

Fig.5 The subcellular localization of ScPRT1 in the epidermal cells of Nicotiana benthamiana leaf The images were taken with fluorescence,bright field,and merge field.35S∷YFP.The transient expression of empty vector pCAMPIA2300-YFP.35S∷ScPRT1∷YFP.The transient expression of vector PCAMBIA2300-ScPRT1-YFP.

图6 ScPRT1与甘蔗黑穗病菌基因共表达网络构建及分析 A.甘蔗栽培品种PRTT与甘蔗黑穗病菌基因共表达网络;B.共表达网络中黑穗病菌效应蛋白结构分析。

Fig.6 Construction and analysis of co-expression network between ScPRT1 and Sporisorium scitamineum A.Co-expression network of S.hybrid cultivar PRT1 and Sporisorium scitamineum genes;B.Structural analysis of effector protein of Sporisorium scitamineum in co-expression network.

参考文献 51

[1]	Arruda P. Genetically modified sugarcane for bioenergy generation[J]. Current Opinion in Biotechnology, 2012, 23(3):315-322.doi:10.1016/j.copbio.2011.10.012. doi: 10.1016/j.copbio.2011.10.012 pmid: 22093808
[2]	班权伦. 甘蔗种植现状和发展对策浅议[J]. 南方农业, 2016, 10(5):29-30.doi:10.19415/j.cnki.1673-890x.2016.15.015. doi: 10.19415/j.cnki.1673-890x.2016.15.015
	Ban Q L. Ban Q L. Discussion on the present situation and development countermeasures of sugarcane planting[J]. South China Agriculture, 2016, 10(5):29-30.
[3]	Que Y X, Su Y C, Guo J L, Wu Q B, Xu L P. A global view of transcriptome dynamics during Sporisorium scitamineum challenge in sugarcane by RNA-Seq[J]. PLoS One, 2014, 9(8):e106476.doi:10.1371/journal.pone.0106476. doi: 10.1371/journal.pone.0106476 URL
[4]	Ling H, Fu X Q, Huang N, Zhong Z F, Su W H, Lin W X, Cui H T, Que Y X. A sugarcane smut fungus effector simulates the host endogenous elicitor peptide to suppress plant immunity[J]. The New Phytologist, 2022, 233(2):919-933.doi:10.1111/nph.17835. doi: 10.1111/nph.17835 URL
[5]	Xu F Q, Xue H W. The ubiquitin-proteasome system in plant responses to environments[J]. Plant,Cell & Environment, 2019, 42(10):2931-2944.doi:10.1111/pce.13633. doi: 10.1111/pce.13633
[6]	Wang J, Qin H, Zhou S R, Wei P C, Zhang H W, Zhou Y, Miao Y C, Huang R F. The ubiquitin-binding protein OsDSK2a mediates seedling growth and salt responses by regulating gibberellin metabolism in rice[J]. The Plant Cell, 2020, 32(2):414-428.doi:10.1105/tpc.19.00593. doi: 10.1105/tpc.19.00593 pmid: 31826965
[7]	Ma X Y, Zhang C, Kim D Y, Huang Y Y, Chatt E, He P, Vierstra R D, Shan L B. Ubiquitylome analysis reveals a central role for the ubiquitin-proteasome system in plant innate immunity[J]. Plant Physiology, 2021, 185(4):1943-1965.doi:10.1093/plphys/kiab011. doi: 10.1093/plphys/kiab011 pmid: 33793954
[8]	Kats I, Khmelinskii A, Kschonsak M, Huber F, Knieβ R A, Bartosik A, Knop M. Mapping degradation signals and pathways in a eukaryotic N-terminome[J]. Molecular Cell, 2018, 70(3):488-501.doi:10.1016/j.molcel.2018.03.033. doi: S1097-2765(18)30236-3 pmid: 29727619
[9]	Timms R T, Zhang Z Q, Rhee D Y, Harper J W, Koren I, Elledge S J. A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation[J]. Science, 2019, 365(6448):eaaw4912.doi:10.1126/science.aaw4912. doi: 10.1126/science.aaw4912 URL
[10]	Deng F Y, Guo T W, Lefebvre M, Scaglione S, Antico C J, Jing T, Yang X, Shan W X, Ramonell K M. Expression and regulation of ATL9,an E3 ubiquitin ligase involved in plant defense[J]. PLoS One, 2017, 12(11):e0188458.doi:10.1371/journal.pone.0188458. doi: 10.1371/journal.pone.0188458 URL
[11]	Li W, Zhong S H, Li G J, Li Q, Mao B Z, Deng Y W, Zhang H J, Zeng L J, Song F M, He Z H. Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence[J]. Cell Research, 2011, 21(5):835-848.doi:10.1038/cr.2011.4. doi: 10.1038/cr.2011.4
[12]	Han P L, Dong Y H, Gu K D, Yu J Q, Hu D G, Hao Y J. The apple U-box E3 ubiquitin ligase MdPUB29 contributes to activate plant immune response to the fungal pathogen Botryosphaeria dothidea[J]. Planta, 2019, 249(4):1177-1188.doi:10.1007/s00425-018-03069-z. doi: 10.1007/s00425-018-03069-z
[13]	李晓辉. 黄瓜泛素E3连接酶基因的克隆及表达分析[D]. 郑州: 河南农业大学, 2014.
	Li X H. Cloning and expression analysis of E3Ubiquitin ligases in Cucumis sativus L.[D]. Zhengzhou: Henan Agricultural University, 2014.
[14]	Gibbs D J, Bailey M, Tedds H M, Holdsworth M J. From start to finish:amino-terminal protein modifications as degradation signals in plants[J]. The New Phytologist, 2016, 211(4):1188-1194.doi:10.1111/nph.14105. doi: 10.1111/nph.14105 URL
[15]	Holdsworth M J, Vicente J, Sharma G, Abbas M, Zubrycka A. The plant N-degron pathways of ubiquitin-mediated proteolysis[J]. Journal of Integrative Plant Biology, 2020, 62(1):70-89.doi:10.1111/jipb.12882. doi: 10.1111/jipb.12882
[16]	Stary S, Yin X J, Potuschak T, Schlögelhofer P, Nizhynska V, Bachmair A. PRT1 of Arabidopsis is a ubiquitin protein ligase of the plant N-end rule pathway with specificity for aromatic amino-terminal residues[J]. Plant Physiology, 2003, 133(3):1360-1366.doi:10.1104/pp.103.029272. doi: 10.1104/pp.103.029272 URL
[17]	Dong H, Dumenil J, Lu F H, Na L, Vanhaeren H, Naumann C, Klecker M, Prior R, Smith C, McKenzie N, Saalbach G, Chen L L, Xia T, Gonzalez N, Seguela M, Inze D, Dissmeyer N, Li Y H, Bevan M W. Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation in Arabidopsis[J]. Genes & Development, 2017, 31(2):197-208.doi:10.1101/gad.292235.116. doi: 10.1101/gad.292235.116 URL
[18]	Chen I H, Chang J E, Wu C Y, Huang Y P, Hsu Y H, Tsai C H. An E3 ubiquitin ligase from Nicotiana benthamiana targets the replicase of Bamboo mosaic virus and restricts its replication[J]. Molecular Plant Pathology, 2019, 20(5):673-684.doi:10.1111/mpp.12784. doi: 10.1111/mpp.12784 pmid: 30924604
[19]	张玉叶, 黄宁, 肖新换, 黄珑, 苏炜华, 许莉萍, 阙友雄. 甘蔗B12D基因全长cDNA克隆与表达分析[J]. 热带生物学报, 2014, 5(2):111-119.doi:10.15886/j.cnki.rdswxb.2014.02.011. doi: 10.15886/j.cnki.rdswxb.2014.02.011
	Zhang Y Y, Huang N, Xiao X H, Huang L, Su W H, Xu L P, Que Y X. Cloning and expression analysis of full cDNA of B12D gene in sugarcane[J]. Journal of Tropical Biology, 2014, 5(2):111-119.
[20]	Ling H, Wu Q B, Guo J L, Xu L P, Que Y X. Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative rt-PCR[J]. PLoS One, 2014, 9(5):e97469.doi:10.1371/journal.pone.0097469. doi: 10.1371/journal.pone.0097469 URL
[21]	Que Y X, Xu L P, Wu Q B, Liu Y F, Ling H, Liu Y H, Zhang Y Y, Guo J L, Su Y C, Chen J B, Wang S S, Zhang C G. Genome sequencing of Sporisorium scitamineum provides insights into the pathogenic mechanisms of sugarcane smut[J]. BMC Genomics, 2014, 15(1):996.doi:10.1186/1471-2164-15-996. doi: 10.1186/1471-2164-15-996 URL
[22]	Zhang J S, Zhang X T, Tang H B, Zhang Q, Hua X T, Ma X K, Zhu F, Jones T, Zhu X, Bowers J, Wai C M, Zheng C F, Shi Y, Chen S, Xu X M, Yue J J, Nelson D R, Huang L X, Li Z, Xu H M, Zhou D, Wang Y J, Hu W C, Lin J S, Deng Y J, Pandey N, Mancini M, Zerpa D, Nguyen J K, Wang L M, Yu L, Xin Y H, Ge L, Arro J, Han J O, Chakrabarty S, Pushko M, Zhang W P, Ma Y H. Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L.[J]. Nature Genetics, 2018, 50(11):1565-1573.doi:10.1038/s41588-018-0237-2. doi: 10.1038/s41588-018-0237-2
[23]	Yang Y Y, Gao S W, Su Y C, Lin Z L, Guo J L, Li M J, Wang Z T, Que Y X, Xu L P. Transcripts and low nitrogen tolerance:Regulatory and metabolic pathways in sugarcane under low nitrogen stress[J]. Environmental and Experimental Botany, 2019, 163:97-111.doi:10.1016/j.envexpbot.2019.04.010. doi: 10.1016/j.envexpbot.2019.04.010 URL
[24]	Chen C J, Chen H, Zhang Y, Thomas H R, Frank M H, He Y H, Xia R. TBtools:An integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant, 2020, 13(8):1194-1202.doi:10.1016/j.molp.2020.06.009. doi: 10.1016/j.molp.2020.06.009 URL
[25]	Letunic I, Khedkar S, Bork P. SMART:Recent updates,new developments and status in 2020[J]. Nucleic Acids Research, 2021, 49(D1):D458-D460.doi:10.1093/nar/gkaa937. doi: 10.1093/nar/gkaa937 pmid: 33104802
[26]	Lescot M, Déhais P, Thijs G, Marchal K, Moreau Y, van de Peer Y, Rouz P, Rombauts S. PlantCARE,a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J]. Nucleic Acids Research, 2002, 30(1):325-327.doi:10.1093/nar/30.1.325. doi: 10.1093/nar/30.1.325 URL
[27]	Wilkins M R, Gasteiger E, Bairoch A, Sanchez J C, Williams K L, Appel R D, Hochstrasser D F. Protein identification and analysis tools in the ExPASy server[J]. Methods in Moleular Biology, 1999, 112:531-552.doi:10.1385/1-59259-584-7:531. doi: 10.1385/1-59259-584-7:531
[28]	Sapay N, Guermeur Y, Deléage G. Prediction of amphipathic in-plane membrane anchors in monotopic proteins using a SVM classifier[J]. BMC Bioinformatics, 2006, 7:255.doi:10.1186/1471-2105-7-255. doi: 10.1186/1471-2105-7-255 pmid: 16704727
[29]	Zhou X G, Li Y, Zhang C X, Zheng W, Zhang G J, Zhang Y. Progressive and accurate assembly of multi-domain protein structures from cryo-EM density maps[J]. Nat Complct Sci, 2022, 2(4):265-275.doi:10.1038/s43588-022-00232-1. doi: 10.1038/s43588-022-00232-1
[30]	Nielsen H, Tsirigos K D, Brunak S, von Heijne G. A brief history of protein sorting prediction[J]. The Protein Journal, 2019, 38(3):200-216.doi:10.1007/s10930-019-09838-3. doi: 10.1007/s10930-019-09838-3
[31]	Nair R, Carter P, Rost B. NLSdb:Database of nuclear localization signals[J]. Nucleic Acids Research, 2003, 31(1):397-399.doi:10.1093/nar/gkg001. doi: 10.1093/nar/gkg001 URL
[32]	黄宁, 惠乾龙, 方振名, 李姗姗, 凌辉, 阙友雄, 袁照年. 甘蔗β-胡萝卜素异构酶基因家族的鉴定、定位和表达分析[J]. 作物学报, 2021, 47(5):882-893.doi:10.3724/SP.J.1006.2021.04128. doi: 10.3724/SP.J.1006.2021.04128
	Huang N, Hui Q L, Fang Z M, Li S S, Ling H, Que Y X, Yuan Z N. Identification,localization and expression analysis of beta-carotene isomerase gene family in sugarcane[J]. Acta Agronomica Sinica, 2021, 47(5):882-893. doi: 10.3724/SP.J.1006.2021.04128 URL
[33]	Huang N, Ling H, Liu F, Su Y C, Su W H, Mao H Y, Zhang X, Wang L, Chen R K, Que Y X. Identification and evaluation of PCR reference genes for host and pathogen in sugarcane-Sporisorium scitamineum interaction system[J]. BMC Genomics, 2018, 19(1):479.doi:10.1186/s12864-018-4854-z. doi: 10.1186/s12864-018-4854-z pmid: 29914370
[34]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using Real-time quantitative PCR and the 2^-ΔΔCT method[J]. Methods, 2001, 25(4):402-408.doi:10.1006/meth.2001.1262. doi: 10.1006/meth.2001.1262 pmid: 11846609
[35]	郑清雷, 余陈静, 姚坤存, 黄宁, 阙友雄, 凌辉, 许莉萍. 甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析[J]. 作物学报, 2020, 46(6):844-857.doi:10.3724/SP.J.1006.2020.94171. doi: 10.3724/SP.J.1006.2020.94171
	Zheng Q L, Yu C J, Yao K C, Huang N, Que Y X, Ling H, Xu L P. Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC[J]. Acta Agronomica Sinica, 2020, 46(6):844-857. doi: 10.3724/SP.J.1006.2020.94171 URL
[36]	Rocafort M, Fudal I, Mesarich C H. Apoplastic effector proteins of plant-associated fungi and oomycetes[J]. Current Opinion in Plant Biology, 2020, 56:9-19.doi:10.1016/j.pbi.2020.02.004. doi: S1369-5266(20)30022-4 pmid: 32247857
[37]	Marchler-Bauer A, Lu S N, Anderson J B, Chitsaz F, Derbyshire M K, Deweese-Scott C, Fong J H, Geer L Y, Geer R C, Gonzales N R, Gwadz M, Hurwitz D I, Jackson J D, Ke Z X, Lanczycki C J, Lu F, Marchler G H, Mullokandov M, Omelchenko M V, Robertson C L, Song J S, Thanki N, Yamashita R A, Zhang D C, Zhang N G, Zheng C J, Bryant S H. CDD:A Conserved Domain Database for the functional annotation of proteins[J]. Nucleic Acids Research, 2010, 39(S1):D225-D229.doi:10.1093/nar/gkq1189. doi: 10.1093/nar/gkq1189 URL
[38]	Berezuk A M, Glavota S, Roach E J, Goodyear M C, Krieger J R, Khursigara C M. Outer membrane lipoprotein RlpA is a novel periplasmic interaction partner of the cell division protein FtsK in Escherichia coli[J]. Scientific Reports, 2018, 8(1):12933.doi:10.1038/s41598-018-30979-5. doi: 10.1038/s41598-018-30979-5 pmid: 30154462
[39]	Jorgenson M A, Chen Y, Yahashiri A, Popham D L, Weiss D S. The bacterial septal ring protein RlpA is a lytic transglycosylase that contributes to rod shape and daughter cell separation in Pseudomonas aeruginosa[J]. Molecular Microbiology, 2014, 93(1):113-128.doi:10.1111/mmi.12643. doi: 10.1111/mmi.12643 pmid: 24806796
[40]	Turek I, Wheeler J, Bartels S, Szczurek J, Wang Y H, Taylor P, Gehring C, Irving H. A natriuretic peptide from Arabidopsis thaliana (AtPNP-A)can modulate catalase 2 activity[J]. Scientific Reports, 2020, 10(1):19632.doi:10.1038/s41598-020-76676-0. doi: 10.1038/s41598-020-76676-0
[41]	Han X W, Altegoer F, Steinchen W, Binnebesel L, Schuhmacher J, Glatter T, Giammarinaro P I, Djamei A, Rensing S A, Reissmann S, Kahmann R, Bange G. A kiwellin disarms the metabolic activity of a secreted fungal virulence factor[J]. Nature, 2019, 565(7741):650-653.doi:10.1038/s41586-018-0857-9. doi: 10.1038/s41586-018-0857-9
[42]	Zylka M J, Sowa N A, Taylor-Blake B, Twomey M A, Herrala A, Voikar V, Vihko P. Prostatic acid phosphatase is an ectonucleotidase and suppresses pain by generating adenosine[J]. Neuron, 2008, 60(1):111-122.doi:10.1016/j.neuron.2008.08.024. doi: 10.1016/j.neuron.2008.08.024 pmid: 18940592
[43]	Labourel A, Frandsen K E H, Zhang F, Brouilly N, Grisel S, Haon M, Ciano L, Ropartz D, Fanuel M, Martin F, Navarro D, Rosso M N, Tandrup T, Bissaro B, Johansen K S, Zerva A, Walton P H, Henrissat B, Leggio L L, Berrin J G. A fungal family of lytic polysaccharide monooxygenase-like copper proteins[J]. Nature Chemical Biology, 2020, 16(3):345-350.doi:10.1038/s41589-019-0438-8. doi: 10.1038/s41589-019-0438-8 pmid: 31932718
[44]	田丽, 包满珠, 张蔚. 植物逆境相关RING finger蛋白的研究进展[J]. 湖北农业科学, 2018, 57(6):5-11,19.doi:10.14088/j.cnki.issn0439-8114.2018.06.001. doi: 10.14088/j.cnki.issn0439-8114.2018.06.001
	Tian L, Bao M Z, Zhang W. Research progress on stress-related RING finger proteins in plants[J]. Hubei Agricultural Sciences, 2018, 57(6):5-11,19.
[45]	Chen M J, Ni M. Red and far-red insensitive 2,a ring-domain zinc finger protein,mediates phytochrome-controlled seedling deetiolation responses[J]. Plant Physiology, 2006, 140(2):457-465.doi:10.1104/pp.105.073163. doi: 10.1104/pp.105.073163 URL
[46]	Liu K M, Wang L, Xu Y Y, Chen N, Ma Q B, Li F, Chong K. Overexpression of OsCOIN,a putative cold inducible zinc finger protein,increased tolerance to chilling,salt and drought,and enhanced proline level in rice[J]. Planta, 2007, 226(4):1007-1016.doi:10.1007/s00425-007-0548-5. doi: 10.1007/s00425-007-0548-5 URL
[47]	Xia Z L, Liu Q J, Wu J Y, Ding J Q. ZmRFP1,the putative ortholog of SDIR1,encodes a RING-H2 E3 ubiquitin ligase and responds to drought stress in an ABA-dependent manner in maize[J]. Gene, 2012, 495(2):146-153.doi:10.1016/j.gene.2011.12.028. doi: 10.1016/j.gene.2011.12.028 URL
[48]	Park C H, Chen S B, Shirsekar G, Zhou B, Khang C H, Songkumarn P, Afzal A J, Ning Y S, Wang R Y, Bellizzi M, Valent B, Wang G L. The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 ubiquitin ligase APIP₆ to suppress pathogen-associated molecular pattern-triggered immunity in rice[J]. The Plant Cell, 2012, 24(11):4748-4762.doi:10.1105/tpc.112.105429. doi: 10.1105/tpc.112.105429 URL
[49]	Ma A F, Zhang D P, Wang G X, Wang K, Li Z, Gao Y H, Li H C, Bian C, Cheng J K, Han Y N, Yang S H, Gong Z Z, Qi J S. Verticillium dahliae effector VDAL protects MYB6 from degradation by interacting with PUB25 and PUB26 E3 ligases to enhance Verticillium wilt resistance[J]. The Plant Cell, 2021, 33(12):3675-3699.doi:10.1093/plcell/koab221. doi: 10.1093/plcell/koab221 URL
[50]	孙伟娜, 徐菁, 殷丽华, 徐晓丹, 柯希望, 左豫虎. 小豆EG45基因的亚细胞定位及其在抗病中的功能分析[M]// 彭友良,王文明,陈雪伟. 中国植物病理学会2019年学术年会论文集. 北京: 中国农业科技出版社, 2019:465.doi:10.26914/c.cnkihy.2019.020468. doi: 10.26914/c.cnkihy.2019.020468
	Sun W N, Xu Q, Yin L H, Xu X D, Ke X W, Zuo Y H. Subcellular localization and functional analysis of Adzuki bean EG 45 gene in disease resistance[M]// Peng Y L,Wang W M,Chen X W. Proceedings of the annual meeting of chinese society for plant pathology. Beijing: China Science and Technology Press, 2019:465.
[51]	Bange G, Altegoer F. Plants strike back:Kiwellin proteins as a modular toolbox for plant defense mechanisms[J]. Communicative & Integrative Biology, 2019, 12(1):31-33.doi:10.1080/19420889.2019.1586049. doi: 10.1080/19420889.2019.1586049

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甘蔗E3泛素连接酶基因PRT1的生物信息学及表达模式分析

Bioinformatics and Expression Pattern Analysis of E3 Ubiquitin Ligase Gene PRT1 in Sugarcane

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