粳稻糖基转移酶Ⅰ基因家族全基因组鉴定及功能分析

doi:10.7668/hbnxb.20193138

摘要/Abstract

摘要：

糖基转移酶Ⅰ(GT Ⅰ)家族是糖基转移酶中的一类,其主要功能涉及黄酮类化合物糖基的转移、淀粉和蔗糖的合成等。为揭示GTⅠ基因家族在粳稻生长发育以及抗逆中的作用,为粳稻GTⅠ基因家族成员的功能研究提供基础,为水稻的品种改良和提高水稻耐高温提供思路,采用生物信息学方法鉴定出30个GTⅠ基因家族成员,并分析了粳稻GTⅠ基因家族成员的基因结构、蛋白质理化性质、染色体定位及基因进化、系统发育关系、保守结构域和蛋白质三维结构建模等。结果显示,粳稻的30个GT Ⅰ基因家族成员在水稻12条染色体上均有分布,其基因结构、保守基序和遗传进化相对保守,家族成员启动子均预测到脱落酸响应元件,家族蛋白质的三维结构保守含有6个β-折叠和7个α-螺旋。通过转录组数据与实时荧光定量分析,GTⅠ基因家族大部分成员对高温胁迫的响应出现在高温处理后第6小时,其中OS-GT29表达量呈持续高表达。GTⅠ基因家族在拟南芥和粳稻功能存在相似。

关键词: 粳稻, 糖基转移酶Ⅰ, 基因家族, 生物信息学, 高温胁迫响应

Abstract:

GlycosyltransferaseⅠ(GT Ⅰ)family is one of the glycosyltransferases,whose main functions involve the glycosyltransferase transfer of flavonoids and the synthesis of starch and sucrose.In order to reveal the role of GTⅠ gene family in the growth and development and high temperature tolerance of japonica rice, and to provide a basis for the functional study of GT Ⅰ gene family members in japonica rice,and provide ideas for rice variety improvement and high temperature tolerance. 30 members of GT Ⅰ gene family were identified by bioinformatics methods.The gene structure,physicochemical properties of proteins,chromosome location,gene evolution,phylogenetic relationship,conserved domain and protein three-dimensional structure modeling of members of GTⅠ genes in japonica rice were analyzed by bioinformatics.The results showed that 30 GT Ⅰ family members of japonica rice were distributed on 12 chromosomes of rice and the promoters of GT Ⅰ family members predicted abscisic acid response.The three-dimensional structure of family proteins was conserved and contained 6 β-folds and 7 α-helix.Transcriptome data and Real-time fluorescence quantitative analysis showed that most members of GT Ⅰ gene family responded to high temperature stress at 6 h after high temperature treatment.The expression level of OS-GT29 was continuously high.GT Ⅰ family was functionally similar in Arabidopsis thaliana and rice.

Key words: Japonica rice, Glycosyltransferase Ⅰ, Gene family, Bioinformatics, High temperature stress response

周镇中, 罗彪, 骆鹰, 夏敏, 汪启明, 饶力群. 粳稻糖基转移酶Ⅰ基因家族全基因组鉴定及功能分析[J]. 华北农学报, 2022, 37(6): 50-62. doi: 10.7668/hbnxb.20193138.

ZHOU Zhenzhong, LUO Biao, LUO Ying, XIA Min, WANG Qiming, RAO Liqun. Genome-wide Identification and Functional Analysis of GlycoltransferaseⅠGene Family in Japonica Rice[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(6): 50-62. doi: 10.7668/hbnxb.20193138.

http://www.hbnxb.net/CN/Y2022/V37/I6/50

图/表 13

参考文献 51

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基因名 Gene name	基因ID Gene ID	正向引物(F)(5'—3') Forward primer(5'—3')	反向引物(R)(5'—3') Reverse primer(5'—3')
OS-GT01	Os04g0589600	TCCTCTCAATTAACCGGTTTGA	CCACCTGCTACTGTTAAGGTTA
OS-GT03	Os01g0262600	CCTCTCTTCCCCAATGGAC	GAGACGGCGATTAGTGGTTAG
OS-GT07	Os06g0229800	GAATTTGCAGAGGATAAGAGCG	CGATCACGTTCATGACATTCTC
OS-GT09	Os07g0412100	GGTAGATTTGCCAGAGAAGACT	GGACTAACCGTCATAACCAGAT
OS-GT11	Os01g0720600	ACTAGTTCTGGGTTTAATGGGG	CATTCCCACAAAAACTAACGGT
OS-GT14	Os11g0158400	ATTCATTTCCAGCAAGTGAACC	CTTTGCACGACACTAGCTATTC
OS-GT16	Os03g0268300	TTGATGTTCGAGTCTATCCTGG	ATTGTACATGTGGCAGTTAGGA
OS-GT19	Os03g0265100	AATGCCTTCAGAATCTGAGACA	CTCATTGCGTATTGTCTTCGAG
OS-GT22	Os11g0236100	GCTTCACTTCACTCTTGCAAAT	TATTAGTGACACGTCTGCTTGT
OS-GT29	Os03g0401300	TCAGCGTATCGAGGAGAAATAC	AATGCTCTCCTCTTTCAGAGTC

基因名 Gene name	基因ID Gene ID	正向引物(F)(5'—3') Forward primer(5'—3')	反向引物(R)(5'—3') Reverse primer(5'—3')
OS-GT01	Os04g0589600	TCCTCTCAATTAACCGGTTTGA	CCACCTGCTACTGTTAAGGTTA
OS-GT03	Os01g0262600	CCTCTCTTCCCCAATGGAC	GAGACGGCGATTAGTGGTTAG
OS-GT07	Os06g0229800	GAATTTGCAGAGGATAAGAGCG	CGATCACGTTCATGACATTCTC
OS-GT09	Os07g0412100	GGTAGATTTGCCAGAGAAGACT	GGACTAACCGTCATAACCAGAT
OS-GT11	Os01g0720600	ACTAGTTCTGGGTTTAATGGGG	CATTCCCACAAAAACTAACGGT
OS-GT14	Os11g0158400	ATTCATTTCCAGCAAGTGAACC	CTTTGCACGACACTAGCTATTC
OS-GT16	Os03g0268300	TTGATGTTCGAGTCTATCCTGG	ATTGTACATGTGGCAGTTAGGA
OS-GT19	Os03g0265100	AATGCCTTCAGAATCTGAGACA	CTCATTGCGTATTGTCTTCGAG
OS-GT22	Os11g0236100	GCTTCACTTCACTCTTGCAAAT	TATTAGTGACACGTCTGCTTGT
OS-GT29	Os03g0401300	TCAGCGTATCGAGGAGAAATAC	AATGCTCTCCTCTTTCAGAGTC

基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT01	Os04g0589600	418	46.58	7.73	质膜
OS-GT02	Os12g0583000	256	29.06	6.74	线粒体
OS-GT03	Os01g0262600	482	52.73	9.45	线粒体
OS-GT04	Os09g0296700	487	53.96	6.38	叶绿体
OS-GT05	Os02g0744700	677	74.15	6.48	叶绿体
OS-GT06	Os10g0437600	749	83.31	5.96	叶绿体
OS-GT07	Os06g0229800	810	88.37	5.14	叶绿体
OS-GT08	Os06g0160700	641	70.95	6.24	叶绿体
OS-GT09	Os07g0412100	608	67.35	6.69	叶绿体
OS-GT10	Os06g0133000	609	66.47	8.22	叶绿体
基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT11	Os01g0720600	975	109.89	5.92	胞质
OS-GT12	Os05g0533600	915	104.18	6.42	胞质
OS-GT13	Os01g0653200	503	55.85	8.48	线粒体
OS-GT14	Os11g0158400	736	82.01	7.25	叶绿体
OS-GT15	Os03g0214400	476	54.17	9.06	胞质
OS-GT16	Os03g0268300	463	53.30	8.27	胞质
OS-GT17	Os07g0100300	479	53.91	9.91	质膜
OS-GT18	Os01g0142300	436	49.15	7.16	胞质
OS-GT19	Os03g0265100	415	46.56	9.28	胞质
OS-GT20	Os07g0270950	471	53.08	7.19	质膜
OS-GT21	Os08g0301500	1 066	118.72	6.35	胞质
OS-GT22	Os11g0236100	509	53.89	7.19	叶绿体
OS-GT23	Os02g0184400	1 011	113.25	7.94	叶绿体
OS-GT24	Os04g0249500	844	96.47	8.09	胞质
OS-GT25	Os04g0309600	844	96.55	7.93	胞质
OS-GT26	Os02g0831500	846	96.15	6.35	胞质
OS-GT27	Os03g0340500	809	92.21	6.52	胞质
OS-GT28	Os07g0616800	816	93.11	6.59	胞质
OS-GT29	Os03g0401300	816	92.90	6.34	胞质
OS-GT30	Os06g0194900	808	92.12	6.35	胞质

基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT01	Os04g0589600	418	46.58	7.73	质膜
OS-GT02	Os12g0583000	256	29.06	6.74	线粒体
OS-GT03	Os01g0262600	482	52.73	9.45	线粒体
OS-GT04	Os09g0296700	487	53.96	6.38	叶绿体
OS-GT05	Os02g0744700	677	74.15	6.48	叶绿体
OS-GT06	Os10g0437600	749	83.31	5.96	叶绿体
OS-GT07	Os06g0229800	810	88.37	5.14	叶绿体
OS-GT08	Os06g0160700	641	70.95	6.24	叶绿体
OS-GT09	Os07g0412100	608	67.35	6.69	叶绿体
OS-GT10	Os06g0133000	609	66.47	8.22	叶绿体
基因名 Gene name	基因ID Gene ID	蛋白质长度/aa Protein length	分子质量/ku Molecular weight	等电点 pI	亚细胞定位 Subcellular localization
OS-GT11	Os01g0720600	975	109.89	5.92	胞质
OS-GT12	Os05g0533600	915	104.18	6.42	胞质
OS-GT13	Os01g0653200	503	55.85	8.48	线粒体
OS-GT14	Os11g0158400	736	82.01	7.25	叶绿体
OS-GT15	Os03g0214400	476	54.17	9.06	胞质
OS-GT16	Os03g0268300	463	53.30	8.27	胞质
OS-GT17	Os07g0100300	479	53.91	9.91	质膜
OS-GT18	Os01g0142300	436	49.15	7.16	胞质
OS-GT19	Os03g0265100	415	46.56	9.28	胞质
OS-GT20	Os07g0270950	471	53.08	7.19	质膜
OS-GT21	Os08g0301500	1 066	118.72	6.35	胞质
OS-GT22	Os11g0236100	509	53.89	7.19	叶绿体
OS-GT23	Os02g0184400	1 011	113.25	7.94	叶绿体
OS-GT24	Os04g0249500	844	96.47	8.09	胞质
OS-GT25	Os04g0309600	844	96.55	7.93	胞质
OS-GT26	Os02g0831500	846	96.15	6.35	胞质
OS-GT27	Os03g0340500	809	92.21	6.52	胞质
OS-GT28	Os07g0616800	816	93.11	6.59	胞质
OS-GT29	Os03g0401300	816	92.90	6.34	胞质
OS-GT30	Os06g0194900	808	92.12	6.35	胞质

组 Group	基因ID组 Gene ID group	非同义突变率 Ka	同义突变率 Ks	核酸分子进化 Ka/Ks	P值 P-value(Fisher)
OS-GT29 vs OS-GT30	Os03g04013 & Os06g01949	0.980	1.067	0.919	0.003
OS-GT29 vs OS-GT25	Os03g04013 & Os04g03096	1.018	0.937	1.087	0.005
OS-GT29 vs OS-GT24	Os03g04013 & Os04g02495	1.019	0.936	1.088	0.005
OS-GT28 vs OS-GT30	Os07g06168 & Os06g01949	0.985	1.051	0.937	0.024
OS-GT28 vs OS-GT25	Os07g06168 & Os04g03096	1.017	0.943	1.078	0.011
OS-GT28 vs OS-GT24	Os07g06168 & Os04g02495	1.016	0.946	1.075	0.015

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