摘要： 在盐、碱胁迫条件下检测控制水稻苗期地上部Na⁺、K⁺浓度的QTL位点，为深入研究水稻的耐盐、碱性遗传机制及分子标记辅助育种提供理论依据。利用小白粳子/空育131衍生的含有200个株系的重组自交系群体，及其包含142个SSR标记的遗传连锁图谱对盐、碱胁迫条件下水稻苗期盐、碱害级别、地上部Na⁺浓度、K⁺浓度及Na⁺/K⁺进行QTL分析。在2种胁迫条件下共定位到了15个QTL，其中盐、碱胁迫条件下分别检测到7，8个QTL，分布在第2，3，5，7，8，9，11，12染色体上，LOD值为2.52~6.59，表型贡献率在6.10%~28.00%。其中，盐胁迫下与Na⁺浓度相关的qSSNC3和碱胁迫下与Na⁺/K⁺相关的qASNK3定位在相邻区间；盐胁迫下与Na⁺/K⁺相关的qSSNK2和碱胁迫下与K⁺浓度相关的qASKC2定位在相同区间；碱胁迫下与碱害级别相关的qSAT8和与Na⁺浓度相关的qASNC8、与K⁺浓度相关的qASKC8定位在相同或相邻区间，说明2种胁迫条件下Na⁺、K⁺浓度具有一定的遗传重叠。通过相同标记或比较图谱将本研究结果与前人研究结果进行比较分析，发现有13个QTL为已知QTL，而位于RM547~RM22750的qSAT8和qASNC8为新的QTL位点。

关键词: 水稻, QTL, 盐碱胁迫, Na⁺, K⁺

Abstract: QTL sites controlling the concentrations of Na⁺ and K⁺ in the upper ground of rice seedlings were detected under salt and alkaline stress conditions to provide a theoretical basis for further research on salt tolerance, alkaline tolerance genetic mechanism and molecular marker assisted breeding of rice. A group of 200 recombinant autogenous lines was derived from Xiaobaijingzi/Kongyu 131 and its genetic linkage map containing 142 SSR marks to analyze the QTL of score of salt and alkali toxicity, Na⁺ concentration, K⁺ concentration and Na⁺/ K⁺ in rice seedling stage under salt and alkaline stress. A total of 15 QTLs were detected under two conditions, seven and eight QTLs were detected under salt and alkaline stress, distributed on chromosomes 2, 3, 5, 7, 8, 9, 11 and 12, LOD values range from 2.52 to 6.59, and phenotype contribution rate ranges from 6.10% to 28.00%. Among them, qSSNC3 associated with Na⁺ concentration under salt stress and qASNK3 associated with Na⁺/ K⁺ under alkaline stress were located in adjacent intervals; qSSNK2 associated with Na⁺/K⁺ under salt stress and qASKC2 associated with K⁺ concentration under alkaline stress were located in the same interval; qSAT8 and qASNC8 related to Na⁺ concentration and qASKC8 related to K⁺ concentration were located in the same or adjacent interval under alkaline stress. The results showed that Na⁺ and K⁺ concentrations had some genetic overlap under two stress conditions. The results of this study were compared with those of previous studies by the same marker or comparative map,13 QTLs were found to be known as QTLs, while qSAT8 and qASNC8, located between RM547-RM22750, were the new QTL sites.

Key words: Rice, QTL, Salt and alkaline stress, Na⁺, K⁺

中图分类号: 

• S511.03