Effect of Enhanced Ammonium Nutrition on Morphological Characteristics and Dry Matter Accumulation of Cotton Seedlings
MA Tongtong1,2, CHEN Li1, WAN Hualong3, LI Jin1, LIU Liantao2, SUN Hongchun2, ZHANG Yongjiang2, BAI Zhiying1, LI Cundong2
1. College of Life Sciences, Hebei Agricultural University, Baoding 071001, China;
2. Key Laboratory of Crop Growth Regulation of Hebei Province, Hebei Agricultural University, Baoding 071001, China;
3. College of Agronomy, Hebei Agricultural University, Baoding 071001, China
Abstract：In order to provide references for nitrogen fertilization in field production, the effects of different NH4+/NO3- ratio of nitrogen nutrition (NH4+-N/NO3--N were 0/100, 25/75, 50/50, 75/25, 100/0)on morphological characteristics and dry matter accumulation, hydroponic experiments were conducted using Nongda 601 as material. The results showed that the plant height, stem diameter, leaf area and main root length of cotton increased gradually with the growth stages changed. In addition to the main root length showed a continuous elongation, the plant height, stem diameter, leaf area and dry weights of cotton increased firstly and then decreased with the increasing of NH4+ concentration in the ammonium nutrient solution. The plant height of cotton seedlings was the highest at the NH4+/NO3- ratio of 50/50 and 25/75 respectively 7, 14, 21 d after enhanced ammonium nutrient solution treatment;both stem diameter and leaf area were the largest at the NH4+/NO3- ratio of 50/50 after enhanced ammonium nutrition treatment 7, 14, 21 d, indicating that the concentration of NH4+ more than 50% could inhibit significantly the growth of cotton seedlings. In terms of dry matter, total dry weight, stem dry weight and leaf dry weight were the highest at the NH4+/NO3- ratio of 75/25, while there was no significant difference in root dry weight and root to shoot ratio, indicating that the effect of enhanced ammonium nutrition on the dry matter weight was positive mainly in the aboveground, while the effect in the underground was not obvious. Moreover, the ratio of root to shoot was the highest, the main root length was the longest when supplied with only NH4+, indicating that single NH4+ could promote root growth. All results showed that ammonium and nitrate nitrogen had different mechanisms for regulating growth and development in cotton underground and aboveground organs, mixed nitrogen was more favorable to the aboveground growth of cotton seedlings.
[1] 喻树迅,范术丽,王寒涛,等.中国棉花高产育种研究进展[J].中国农业科学,2016,49(18):3465-3476.
[2] 林兴军,陈鹏,孙燕,等.不同形态氮素及铵硝比例对咖啡氮吸收和生长的影响[J].热带农业科学,2016,36(7):17-21.
[3] 顾红梅,邓光华,翁贤权,等.丝栗栲幼苗对不同氮含量及形态的营养响应[J].江西农业大学学报,2016,38(2):312-318.
[4] 孙敏红,卢晓鹏,李静,等.不同氮素形态对枳橙幼苗生长特性的影响[J].湖北农业科学,2016,55(8):2014-2018,2022.
[5] 卢颖林.铵硝营养影响番茄幼苗生长和氮素代谢的分子生理机制[D].南京:南京农业大学,1998:488-492.
[6] 戴廷波,曹卫星,李存东.作物增铵营养的生理效应[J].植物生理学通讯,1998,6(34):488-492.
[7] 胡琳莉,廖伟彪,马彦霞,等.弱光下不同铵硝配比氮素对大白菜幼苗生长和抗氧化的影响[J].园艺学报,2016,43(5):897-906.
[8] Sun H J,Zhang H L,Powlson D,et al. Rice production,nitrous oxide emission and ammonia volatilization as impacted by the nitrification inhibitor 2-chloro-6-(trichloromethyl)-pyridine[J]. Field Crops Research,2015,173(9):1-7.
[9] Duan Y H,Yin X M,Zhang Y L,et al. Mechanisms of enhanced rice growth and nitrogen uptake by nitrate[J]. Pedosphere,2007,17(6):697-705.
[10] 张淑英,褚贵新,梁永超.增铵营养对低温胁迫下棉花幼苗氮代谢的影响[J].植物营养与肥料学报,2017,23(4):983-990.
[11] 钟丽华,宋世威,刘厚诚,等.不同铵硝配比对芥蓝产量和品质的影响[J].中国蔬菜,2012,1(4):63-67.
[12] Tian Z,Wang J J,Liu S,et al. Application effects of coated urea and urease and nitrification inhibitors on ammonia and greenhouse gas emissions from a subtropical cotton field of the Mississippi delta region[J]. Science of the Total Environment,2015,533:329-338.
[13] Liu C,Wang K,Zheng X. Effects of nitrification inhibitors(DCD and DMPP)on nitrous oxide emission,crop yield and nitrogen uptake in a wheat-maize cropping system[J]. Biogeosciences,2013,10(4):2427-2437.
[14] Soares J R,Cantarella H,De Campos Menegale M L. Ammonia volatilization losses from surface-applied urea with urease and nitrification inhibitors[J]. Soil Biology & Biochemistry,2012,52(8):82-89.
[15] Zaman M,Saggar S,Blennerhassett J D,et al. Effect of urease and nitrification inhibitors on N transformation,gaseous emissions of ammonia and nitrous oxide,pasture yield and N uptake in grazed pasture system[J]. Soil Biology & Biochemistry,2009,41(6):1270-1280.
[16] Hammer P A,Tibbitts T W,Langhans R W,et al. Base-line growth studies of grand rapids lettuce in controlled environments[J]. Journal American Society for Horticultural Science,1978,103(5):649-655.
[17] 刘英杰,李友军,吴金芝,等.田间增铵条件下不同基因型小麦早期生长的差异研究[J].河南农业科学,2012,41(6):29-33.
[18] 赵丽莉,邓光存,吴晓玲.不同铵态氮和硝态氮配比对黄芩幼苗生长及生理特性的影响[J].北方园艺,2010(5):191-193.
[19] 董宝珠,李宏光,杨红武,等.不同氮素形态及配比营养液对烟苗生长及生理特性的影响[J].作物研究,2015,29(1):26-31,36.
[20] 宋世威,章笑潫,刘厚诚,等.不同铵硝配比对芥蓝品质影响的综合评价[J].热带作物学报,2015,36(8):1525-1529.
[21] 王岚,黄承和,陈玉子,等.铵硝配比对巴西香蕉生长和氮素营养的影响[J].热带作物学报,2016,37(1):26-29.
[22] 雷玉玲,陈紫妍,宋世威,等.不同铵硝配比对叶用莴苣生长及氮磷钾养分积累的影响[J].中国蔬菜,2015,1(9):48-52.
[23] 杨阳,郑秋玲,裴成国,等.不同硝铵比对霞多丽葡萄幼苗生长和氮素营养的影响[J].植物营养与肥料学报,2010,16(2):370-375.
[24] 李凯,郭宇琦,刘楚楠,等.铵硝配比对大豆生长及结瘤固氮的影响[J].中国油料作物学报,2014,36(3):349-356.
[25] Chen W,Luo J K,Shen Q R. Effect of NH4+-N/NO3--N ratios on growth and some physiological parameters of Chinese cabbage cultivars[J]. Pedosphere,2005,15(3):310-318.
[26] 乔旭,褚贵新,梁永超,等. NaCl胁迫下不同铵硝配比对棉花苗期生长发育的影响[J].新疆农业科学,2009,46(6):1206-1211.
[27] 邓兰生,齐庆振,龚林,等.滴施不同铵硝比例氮肥对马铃薯生长的影响[J].中国土壤与肥料,2011(4):13-16.
2018, Vol. 33 
