摘要： 为了阐明红麻耐盐机理，以耐盐性较强的中红麻16号为材料，从红麻耐盐转录组测序结果中获得了1个与AVP1基因高度相似的Unigene，根据该片段的序列设计引物，直接进行PCR扩增，经Sanger测序获得该基因全长cDNA序列。对该基因序列进行生物信息学分析表明：该基因全长2 298 bp，开放阅读框为2 298 bp，编码765个氨基酸，其编码蛋白质的分子量和等电点分别为80.2 kDa和5.25，与雷蒙德氏棉、甜橙、毛果杨、烟草和大豆的H⁺-PPase基因核苷酸序列的相似性分别为90%，85%，85%，83%，83%，蛋白质序列的相似性分别为96%，93%，91%，93%，94%，说明该基因与AVP1为同源基因，命名为 Hcvp1。qRT-PCR分析表明，该基因的表达量随着NaCl浓度的增加而增加。这将为Hcvp1基因的功能验证和红麻耐盐机理的研究奠定坚实的基础。

关键词: 红麻, H⁺-PPase基因, 耐盐

Abstract: In order to elucidate the mechanism of salt tolerance, taking Zhonghongma 16 with salinity tolerance as an experimental material, we obtained a kenaf Unigene highly identified with AVP1 gene from the kenaf transcriptome sequence. According to the known fragment, we developed primer to amplify directly by PCR, and the cDNA sequence of the gene was obtained by Sanger sequencing. The results of bioinformatics analysis showed that the full length of the Hcvp1 gene was 2 298 bp, the open reading frame was 2 298 bp, coding 765 amino acids, the molecular weight and the isoelectric point of coded protein were 80.2 kDa and 5.25.Nucleotide sequence Blast indicated that the Unigene of kenaf shared an identity of was 90%, 85%, 85%, 83%, 83%,with AVP1 of Gossypium raimondii, Citrus sinensis, Populus trichocarpa, Nicotiana tabacum, Glycine max, respectively, and the similarity in protein Blast was 96%, 93%, 91%, 93%, 94% respectively. The results showed that it was a homologous gene of AVP1 in kenaf and named Hcvp1.qRT-PCR analysis indicated that the expression level of Hcvp1 went up while the rise of NaCl concentration. This study will lay a solid foundation for the Hcvp1 gene function validation and the research of salt tolerance mechanism in kenaf.

Key words: Kenaf, H⁺-PPase gene, Salinity tolerance

中图分类号: 

• Q78