小麦类钙调素<em>TaCML79</em>基因的克隆和表达分析

doi:10.7668/hbnxb.2016.06.001

华北农学报 ›› 2016, Vol. 31 ›› Issue (6): 1-6. doi: 10.7668/hbnxb.2016.06.001

所属专题： 小麦；生物技术；

小麦类钙调素TaCML79基因的克隆和表达分析

周硕, 刘永伟, 董福双, 杨帆, 赵和, 柴建芳, 吕孟雨, 孙果忠, 王海波

河北省农林科学院遗传生理研究所, 河北省植物转基因中心, 河北石家庄 050051

收稿日期:2016-08-26 出版日期:2016-12-28
通讯作者: 王海波(1958-),男,河北保定人,研究员,博士,主要从事作物遗传、生理、育种及转基因研究。
作者简介:周硕(1985-),男,河北邢台人,助理研究员,博士,主要从事作物分子生物学研究。
基金资助:
河北省农林科学院博士基金项目（F15E0008）；北京全式金生物技术有限公司Trans助研梦想基金（Trans-RasDF-003）；国家自然科学基金项目（31600216）；河北省农林科学院科学技术研究与发展计划项目（A2015110102）

Cloning and Expression Analysis of TaCML79 in Wheat

ZHOU Shuo, LIU Yongwei, DONG Fushuang, YANG Fan, ZHAO He, CHAI Jianfang, LÜ Mengyu, SUN Guozhong, WANG Haibo

Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China

Received:2016-08-26 Published:2016-12-28

摘要/Abstract

摘要： 为了研究小麦类钙调素基因的功能和在植物逆境响应中的分子机制，以电子克隆结合RT-PCR的方法，在小麦中克隆到一个类钙调素基因TaCML79。该基因的开放阅读框长度为588 bp，编码的蛋白长度为195个氨基酸，推测的分子量为20.45 kDa，等电点为4.6，属于酸性蛋白，具有2个典型的和一个不完整的EF-hand结构域。多序列比对和系统进化树分析表明，TaCML79与野生稻的ObCML35和水稻的OsCML10亲缘关系较近，相似性分别为84.6%和86.8%。该基因的表达在根和地上部分受到热激、NaCl、渗透和冷胁迫的诱导或抑制，初步推断该基因可能与植物抗逆有密切的关系。

关键词: 小麦, 类钙调素, EF-hand结构域, 基因克隆, 基因表达

Abstract: In order to study the function and molecular mechanism of wheat calmodulin-like protein gene(CML) in plant stress response,one CML gene TaCML79 was cloned in wheat based on electron cloning and RT-PCR.Sequence analysis showed that the open reading frame (ORF) of TaCML79 was 588 bp,encoding 195 amino acids of protein containing two typical and one incomplete EF-hand domains,with predicted molecular weight of 20.45 kDa and isoelectric point of 4.6.Multiple sequence alignment and phylogenetic tree analysis showed that TaCML79 was closely related to the ObCML35 and OsCML10,with 84.6% and 86.8% identity respectively.The expression of TaCML79 was induced or inhibited by heat shock,NaCl,osmotic and cold stress in root and shoot tissue,suggesting that TaCML79 might be closely related to plant resistance to abiotic stress.

Key words: Triticum aestivum, Calmodulin-like proteins, EF-hand domain, Gene cloning, Gene expression

中图分类号:

Q78
S512.1

周硕, 刘永伟, 董福双, 杨帆, 赵和, 柴建芳, 吕孟雨, 孙果忠, 王海波. 小麦类钙调素TaCML79基因的克隆和表达分析[J]. 华北农学报, 2016, 31(6): 1-6. doi: 10.7668/hbnxb.2016.06.001.

ZHOU Shuo, LIU Yongwei, DONG Fushuang, YANG Fan, ZHAO He, CHAI Jianfang, LÜ Mengyu, SUN Guozhong, WANG Haibo. Cloning and Expression Analysis of TaCML79 in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 1-6. doi: 10.7668/hbnxb.2016.06.001.

http://www.hbnxb.net/CN/Y2016/V31/I6/1

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