低温后不同光强对烤烟幼苗叶片活性氧代谢和叶绿素荧光参数的影响

doi:10.7668/hbnxb.2015.S1.040

摘要/Abstract

摘要： 为揭示低温和光强对我国北方烤烟幼苗移栽期生长的影响,采用室内盆栽方法,模拟研究我国北方早春低温(8±2)℃和光强(模拟弱光:200 μmol/(m²·s);模拟强光:1 000 μmol/(m²·s))不同组合,对移栽期烤烟幼苗叶片活性氧代谢和叶绿素荧光参数的影响.结果表明:低温胁迫1,2 h后弱光处理下烤烟幼苗叶片的O₂^·速率没有明显变化,但低温后强光处理下O₂^·产生速率明显增加,低温后不同光强下烤烟幼苗叶片的H₂O₂含量均明显增加,并且低温胁迫1,2 h后强光处理下O₂^·产生速率和H₂O₂含量增加幅度均明显大于弱光处理.低温胁迫1,2 h后200 μmol/(m²·s)光强处理下以及低温胁迫1 h后强光处理下烤烟幼苗叶片的SOD和APX活性明显增加,但低温胁迫2 h后强光处理下SOD和APX活性却有所降低,即(8±2)℃低温胁迫2 h后强光处理抑制了烤烟幼苗叶片抗氧化酶的活性.不同处理下烤烟幼苗叶片活性氧的变化导致了叶片MDA大量积累,膜质过氧化程度的增加,同样MDA含量的变化幅度也表现为强光处理大于弱光处理,低温处理2 h大于低温处理1 h.低温1,2 h后弱光下烤烟幼苗叶片的Fv/Fm和Fv/Fo没有发生明显变化,但低温后强光下Fv/Fm和Fv/Fo却明显降低,同样低温2 h处理的降低幅度均大于低温1 h处理.

关键词: 低温, 光强, 烤烟, 活性氧, 叶绿素荧光

Abstract: In this paper,the changes of reactive oxygen species and chlorophyll fluorescence parameters in leaves of flue-curing tobacco seedlings in different combinations of simulating low temperature (8±2)℃ and light intensity(weak light:200 μmol/(m²·s),strong light:1 000 μmol/(m²·s)) in the early spring of northern China after chilling stress were studied,the aim was to exploit response mechanism of regular low temperature and strong light on survival and growth of transplanting tobacco seedlings.The results showed that after low temperature for 1,2 h,the rates of production of O₂^· had not changed significantly in the weak light treatment,while,the rates of production of O₂^· had increased significantly in the treatment of strong light.Meanwhile the produce rate of O₂^· and increasing contents of H₂O₂ in strong light were obviously higher than in weak light.The activity of SOD and APX were increased significantly in weak light after low temperature for 1,2 h and in strong light after low temperature for 1 h,while,the activity of SOD and APX were decreased mostly in the strong light after low temperature for 2 h.The changes of reactivity oxygen species in tobacco leaves under different stress resulted in the numerous MDA accumulation,the extent of membranous peroxide had been increased.Samely,the MDA contents in strong light treatment were more than in weak light treatment,and the MDA contents in low temperature for 2 h were more than for 1 h.The parameters of Fv/Fm and Fv/Fo were not changed significantly in weak light after low temperature for 1,2 h,while,parameters of Fv/Fm and Fv/Fo were decreased mostly in the strong light after low temperature,and degree of decreasing in low temperature for 2 h were more than for 1 h.

Key words: Low temperature, Light intensities, Flue-curing tobacco, Reactive oxygen species, Chlorophyll fluorescence

中图分类号:

郭瑞, 曾榕, 张金鑫, 张秀丽, 孙广玉. 低温后不同光强对烤烟幼苗叶片活性氧代谢和叶绿素荧光参数的影响[J]. 华北农学报, 2015, 30(S1): 225-230. doi: 10.7668/hbnxb.2015.S1.040.

GUO Rui, ZENG Rong, ZHANG Jin-xin, ZHANG Xiu-li, SUN Guang-yu. Effects of Different Light Intensities on Reactive Oxygen Species Metabolism and Chlorophyll Fluorescence Parameters in Leaves of Flue-curing Tobacco Seedlings after Chilling Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 225-230. doi: 10.7668/hbnxb.2015.S1.040.

http://www.hbnxb.net/CN/Y2015/V30/IS1/225

参考文献

[1] 张会慧,包卓,许楠,等.钙对低温高光锻炼下烤烟幼苗光合的促进效应[J].核农学报,2011,25(3):582-587.
[2] Ben-Hua J I,Zhu S Q,Jiao D M.Photochemical Efficiency of PSⅡ and Membrane Lipid Peroxidation in Leaves of indica and japonica Rice (Oryza sativa) Under Chilling Temperature and Strong Light Stress Conditions[J].Acta Botanica Sinica,2002,44(2):139-146.
[3] Long S P,Humphries S,Falkowski P G.Photoinhibition of photosynthesis in nature[J].Annual Review of Plant Physiology and Plant MolecularBiology,1994,45:633-662.
[4] Egerton J G,Banks J C,Gibson A,et al.Facilitation of seedling establishment:Reduction in irradiance enhances wintergrowth of Eucalyptus pauciflora[J].Ecology,2000,81(5):1437-1449.
[5] 张会慧,田褀,刘关君,等.转 2-Cys Prx 基因烟草抗氧化酶和PSⅡ电子传递对盐和光胁迫的响应[J].作物学报,2013,39(11):2023-2029.
[6] 包卓.低温高光锻炼下烤烟幼苗叶片光合适应性的初步研究[D].哈尔滨:东北林业大学,2010.
[7] 樊传辉,孙广玉,吕清友.光胁迫下烤烟幼苗叶片光抑制及Ca²⁺的缓解作用[J].东北林业大学学报,2007,06(6):34-36.
[8] 招启柏,吕冰,王广志,等.苗期低温对烤烟叶数及现蕾时间的影响[J].中国烟草学报,2008(3):27-31,35.
[9] 宗学凤,王三根.植物生理研究技术[M].重庆:西南师范大学出版社,2011.
[10] 汤绍虎,罗充.植物生理学实验教程[M].重庆:西南师范大学出版社,2012.
[11] 王晶英,敖红,张杰,等.植物生理生化实验技术与原理[M].哈尔滨:东北林业大学出版社,2003.
[12] Hu Y B,Sun G Y,Wang X C.Induction characteristics and response of photosynthetic quantum conversion to changes in irradiance in mulberry plants[J].Journal of Plant Physiology,2007,164(8):959-968.
[13] Apel K,Hirt H.Reactive oxygen species:metabolism,oxidative stress,and signal transduction[J].Annual Review of Plant Biology, 2004,55(1):373-399.
[14] Takahashi M,Asada K.Superoxide production in aprotic interior of chloroplast thylakoids[J].Archives of Biochemistry and Biophysics,1988,267(2):714-722.
[15] 逄好胜,张会慧,田野,等.不同光强下盐胁迫对桑树幼苗叶片活性氧和叶绿素荧光特性的影响[J].中南林业科技大学学报,2014,34(8):42-47.
[16] 孙卫红,王伟青,孟庆伟.植物抗坏血酸过氧化物酶的作用机制、酶学及分子特性[J].植物生理学通讯,2005,41(2):143-147.
[17] 杨春武,李长有,尹红娟,等.小冰麦(Triticum aestivum-Agropyron intermedium)对盐胁迫和碱胁迫的生理响应[J].作物学报,2007,33(8):1255-1261.
[18] 郑春芳,姜东,戴廷波,等.外源一氧化氮供体硝普钠浸种对盐胁迫下小麦幼苗碳氮代谢及抗氧化系统的影响[J].生态学报,2010,30(5):1174-1183.
[19] 江文文,尹燕枰,王振林,等.花后高温胁迫下氮肥追施后移对小麦产量及旗叶生理特性的影响[J].作物学报,2014,40(5):942-949.
[20] 王仁雷,华春,刘友良.叶绿体中的水-水循环[J].植物生理学通讯,2001,37(5):468-470.
[21] Aran M,Ferrero D S,Pagano E,et al.Typical 2-Cys peroxiredoxins-modulation by covalent transformations and noncovalent interactions[J].The FEBS Journal,2009,276(9):2478-2493.
[22] 马凤鸣,王瑞,石振.低温胁迫对玉米幼苗某些生理指标的影响[J].作物杂志,2007(5):41-45.
[23] 郝小琴,姚鹏鹤,高峥荣,等.低温胁迫对微胚乳超甜超高油玉米耐寒性生理生化特性的影响[J].作物学报,2014,40(8):1470-1484.
[24] 田野,郑桂英,张会慧,等.光强和温度对罗勒幼苗叶片叶绿素荧光的影响[J].草业科学,2013,30(10):1561-1568.
[25] 蔡志全,曹坤芳,冯玉龙,等.夜间低温胁迫对两种生长光强下藤黄幼苗叶片荧光特征和活性氧代谢的影响[J].应用生态学报,2003,14(3):326-330.

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