基于高代回交分离群体的小麦抗赤霉病QTL <i>Fhb4</i>和<i>Fhb5</i>的遗传互作模式分析

doi:10.7668/hbnxb.2015.05.005

摘要/Abstract

摘要： 为了明确2个小麦抗赤霉病侵入的主效QTL Fhb4 和 Fhb5 的抗性遗传和互作模式,采用土表法并结合扬花期喷洒孢子液接种,以病小穗率(PDS)为鉴定指标,对携带 Fhb4 和 Fhb5 的不同遗传背景的BC₃F₁、BC₃F₂以及抗感对照进行了抗赤霉病侵入的表型鉴定和评价。结果表明, Fhb4 和 Fhb5 在温麦6号和周麦22这2个不同的遗传背景中,PDS表现出杂合基因型和供体亲本望水白基因型的差异不显著,而与轮回亲本基因型之间的差异达到显著水平,抗感分离比经卡方检验符合9:3:3:1的分离比例,其抗性遗传遵循2个独立的遗传因子控制的显性遗传模式;同时, Fhb4 和 Fhb5 抗侵入性效应相当,它们之间存在加性效应,二者累加后的病小穗率显著低于单个 Fhb4 或 Fhb5。因此,在育种实践中对这2个抗赤霉病显性基因的聚合利用将有助于提高育种材料的基础抗性。

关键词: 小麦, 赤霉病, 抗病性, 回交, 病小穗率

Abstract: For characterizing the genetics interactions between Fhb4 and Fhb5,during the BC₃F₁ and BC₃F₂ generations,the wheat lines carried this two QTLs had been identified and evaluated for the resistance to wheat scab under surface of soil inoculation conditions combined with spraying spores compared to CK-R and CK-S,according to percentage of diseased spikelet.The results showed that there was no significant difference between heterozygous genotypes and donor parents in contrast to recurrent parents under Wenmai 6 and Zhoumai 22 genetic background,respectively.Furthermore,the resistance segregation was 9:3:3:1,so Fhb4 and Fhb5 were two independent of each other dominant Mendelian inheritance factor.Moreover,there was a notable additive effect between Fhb4 and Fhb5 when they were accumulated in individual plant,and presented similar resistance effect to penetration.Therefore,pyramiding Fhb4 and Fhb5 would improve the basal resistance to FHB in breeding practice.

Key words: Wheat, Fusarium head blight, Disease resistance, Backcross, Percentage of diseased spikelet

中图分类号:

许峰, 闫素辉, 张从宇, 时侠清, 李文阳, 张子学. 基于高代回交分离群体的小麦抗赤霉病QTL Fhb4和Fhb5的遗传互作模式分析[J]. 华北农学报, 2015, 30(5): 30-35. doi: 10.7668/hbnxb.2015.05.005.

XU Feng, YAN Su-hui, ZHANG Cong-yu, SHI Xia-qing, LI Wen-yang, ZHANG Zi-xue. Resistance to Fusarium Head Blight in Wheat Genetic Model Analysis of Major Effect QTL Fhb4 and Fhb5[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 30-35. doi: 10.7668/hbnxb.2015.05.005.

http://www.hbnxb.net/CN/Y2015/V30/I5/30

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基于高代回交分离群体的小麦抗赤霉病QTL Fhb4和Fhb5的遗传互作模式分析

Resistance to Fusarium Head Blight in Wheat Genetic Model Analysis of Major Effect QTL Fhb4 and Fhb5

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基于高代回交分离群体的小麦抗赤霉病QTL Fhb4和Fhb5的遗传互作模式分析

Resistance to Fusarium Head Blight in Wheat Genetic Model Analysis of Major Effect QTL Fhb4 and Fhb5

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