黄淮麦区南片小麦Glu-3位点等位变异分析

doi:10.7668/hbnxb.2015.04.005

华北农学报 ›› 2015, Vol. 30 ›› Issue (4): 25-30. doi: 10.7668/hbnxb.2015.04.005

所属专题： 小麦；

黄淮麦区南片小麦Glu-3位点等位变异分析

陈蕴文, 张莉莉, 余欣欣, 马守才, 郑锦娟, 薛小雁, 郑雅潞, 牛娜, 王军卫, 张改生

西北农林科技大学, 国家杨凌农业生物技术育种中心, 国家小麦改良中心杨凌分中心, 小麦育种教育部工程研究中心, 陕西省作物杂种优势研究与利用重点实验室, 陕西杨凌 712100

收稿日期:2015-04-07 出版日期:2015-08-28
通讯作者: 马守才(1968-),男,陕西大荔人,副教授,博士,硕士生导师,主要从事小麦杂种优势利用与种子工程研究。
作者简介:陈蕴文(1990-),女,河北秦皇岛人,硕士,主要从事小麦杂种优势利用研究。
基金资助:
国家“863”计划项目(2011AA10106);陕西省自然科学基金项目(2012JM3003);公益性行业(农业)科研专项(201303002);西北农林科技大学唐仲英基金项目(A212021204)

Allelic Variation of Glu-3 in Wheat from South Yellow and Huai Valley Facultative Wheat Region in China

CHEN Yun-wen, ZHANG Li-li, YU Xin-xin, MA Shou-cai, ZHENG Jin-juan, XUE Xiao-yan, ZHENG Ya-lu, NIU Na, WANG Jun-wei, ZHANG Gai-sheng

Northwest A&F University, National Yangling Agricultural Biotechnology & Breeding Center, Yangling Branch of State Wheat Improvement Center, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Yangling 712100, China

Received:2015-04-07 Published:2015-08-28

摘要/Abstract

摘要： 小麦的加工品质与低分子量谷蛋白亚基(LMW-GS)组成息息相关。为了尽快改良黄淮麦区小麦加工品质,应用STS分子标记与SDS-PAGE相结合的方法对小麦低分子量谷蛋白亚基Glu-A3、Glu-B3与Glu-D3位点的等位基因变异类型进行检测。结果表明:黄淮麦区南片356份小麦品种(系)中共检测到15种Glu-3位点等位变异,Glu-A3位点含Glu-A3a、Glu-A3b、Glu-A3c和Glu-A3d共4种等位变异,其分布频率分别为12.1%,13.5%,41.0%,33.4%;Glu-B3位点含Glu-B3a、Glu-B3b、Glu-B3d、Glu-B3f、Glu-B3g、Glu-B3h、Glu-B3i和Glu-B3j共8种等位变异,其分布频率分别为8.71%,8.99%,23.0%,5.90%,7.30%,3.65%,0.28%,42.1%;Glu-D3位点含Glu-D3a、Glu-D3b和Glu-D3c共3种等位变异,其分布频率分别为40.2%,29.8%,30.0%。等位变异组合Glu-A3c/Glu-B3j/Glu-D3a分布频率最高(10.7%)。通过优质亚基的转育,多个优质亚基的聚合,将有助于改良我国小麦的品质。

关键词: 小麦, STS分子标记, Glu-A3, Glu-B3, Glu-D3

Abstract: Low-molecular-weight glutenin subunits (LMW-GS) are closely related to processing quality of wheat.The alleles on Glu-A3,Glu-B3 and Glu-D3 loci of low-molecular-weight glutenin subunit in 356 wheat varities(lines) from South Yellow and Huai Valley Facultative Wheat region in China were detected by STS molecular markers and SDS-PAGE to improve wheat quality.It turned out that:There were 15 kinds of allelic variations in 356 detected varieties.The frequencies of the allelic variations of Glu-A3a,Glu-A3b,Glu-A3c and Glu-A3d at Glu-A3 locus were 12.1%,13.5%,41.0%,33.4%,respectively;the alleles of Glu-B3a,Glu-B3b,Glu-B3d,Glu-B3f,Glu-B3g,Glu-B3h,Glu-B3i and Glu-B3j at Glu-B3 locus were 8.71%,8.99%,23.0%,5.90%,7.30%,3.65%,0.28%,42.1%,respectively;and the alleles of Glu-D3a,Glu-D3b and Glu-D3c at Glu-D3 locus were 40.2%,29.8%,30.0%,respectively.The highest frequency of the subunit combination at Glu-3 was Glu-A3c/Glu-B3j/Glu-D3a (10.7%).Transformating and pyramiding superior subunits can be helpful in Chinese wheat quality improvement.

Key words: Ttiticum aestivum L., STS molecular markers, Glu-A3, Glu-B3, Glu-D3

中图分类号:

S512.03

陈蕴文, 张莉莉, 余欣欣, 马守才, 郑锦娟, 薛小雁, 郑雅潞, 牛娜, 王军卫, 张改生. 黄淮麦区南片小麦Glu-3位点等位变异分析[J]. 华北农学报, 2015, 30(4): 25-30. doi: 10.7668/hbnxb.2015.04.005.

CHEN Yun-wen, ZHANG Li-li, YU Xin-xin, MA Shou-cai, ZHENG Jin-juan, XUE Xiao-yan, ZHENG Ya-lu, NIU Na, WANG Jun-wei, ZHANG Gai-sheng. Allelic Variation of Glu-3 in Wheat from South Yellow and Huai Valley Facultative Wheat Region in China[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(4): 25-30. doi: 10.7668/hbnxb.2015.04.005.

http://www.hbnxb.net/CN/Y2015/V30/I4/25

参考文献

[1] Bietz J A,Wall J S.Isolation and characterization of gliadin like subunits from glutenin[J].Soil Biology & Biochemistry,1973,50(5):537-547.
[2] Liu L,He Z H,Yan J,et al.Allelic variation at the Glu-1 and Glu-3 loci,presence of the 1B.1R translocation,and their effects on Mixographic properties in Chinese bread wheats[J].Euphytica,2005,142(3):197-204.
[3] Vazquez J F,Ruiz M,Nizto-Taladriz,et al.Effects on gluten strength of low M(r) glutenin subunits coded by alleles at Glu-A3 and Glu-B3 loci in durum wheat[J].Cereal Science,1996,24(2):125-130.
[4] Gupta R B,MacRitchie F.Allelic variation at glutenin subunit and gliadin loci,Glu-1,Glu-3 and Gli-1 of common wheats.II.biochemical basis of the allelic effects on dough properties[J].Cereal Science,1994,19(1):19-29.
[5] Brandlard G,Felix I.Part of the HMW glutenin subunits and omega-gliadin allelic variants in the explanation of the quality parameters[M]//Proceeding International Meeting-Wheat Kernel Proteins:Molecular and Functional Aspects,S.Viterbo,Italy:Martinal al Cimino,1994:249-251.
[6] Zhang W,Gianibelli M C,Rampling L R,et al.Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum L.)[J].Theoretical and applied genetics,2004,108(7):1409-1419.
[7] Wang L H,Zhao X L,He Z H,et al.Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.)[J].Theoretical and Applied Genetics,2009,118(3):525-539.
[8] Wang L H,Li G Y,Pea R J,et al.Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat(Triticum aestivum L.) [J].Cereal Science,2010,51(3):305-312.
[9] 刘建军,何中虎,Pena R J,等.1BL/1RS易位对小麦加工品质的影响[J].作物学报,2004,30(2):149-153.
[10] Froidmont D.A co-dominant marker for the 1BL/1RS wheat-rye translocation via Multiplex PCR[J].Cereal Science,1998,27(3):229-232.
[11] Chai J F,Zhou R H,Jia J Z,et al.Development and application of a new codominant PCR marker for detecting 1BL center dot 1RS wheat-rye chromosome translocations[J].Plant Breeding,2006,125(3):302-304.
[12] 刘丽,周阳,何中虎,等.高、低分子量麦谷蛋白亚基等位变异对小麦加工品质性状的影响[J].中国农业科学,2004,37(1):8-14.
[13] Gupta R B,Shepherd K W.Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutelin:1.Variation and genetic control of the subunits in hexaploid wheats[J].Theoretical and Applied Genetics,1990,80(1):65-74.
[14] Branlard G,Dardevet M,Amiour N,et al.Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat (Triticum aestivum L.) [J].Genetic Resources and Crop Evolution,2003,50(7):669-679.
[15] 刘丽,于亚雄,杨金华,等.小麦Glu-3位点编码亚基的研究进展[J].云南农业大学学报,2004,19(2):138-143.
[16] D'ovidio R,Simeone M,Masci S,et al.Molecular characterization of a LMW-GS gene located on chromosome 1B and the development of primers specific for the Glu-B3 complex locus in durum wheat[J].Theoretical and Applied Genetics,1997,95(7):1119-1126.
[17] 赵惠贤,郭蔼光,胡胜武,等.小麦Glu-D3和Glu-B3位点LMW-GS基因特异引物设计与PCR扩增[J].作物学报,2004,30(2):126-130.
[18] Zhang W,Gianibelli M C,Rampling L,et al.Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum L.) [J].Theoretical and Applied Genetics,2004,108(7):1409-1419.
[19] Wang L H,Zhao X L,He Z H,et al.Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.) [J].Theoretical and Applied Genetics,2009,118(3):525-539.
[20] Wang L H,Li G Y,Peña R J,et al.Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.)[J].Cereal Science,2010,51(3):305-312.
[21] 孙学永,马传喜,司红起,等.中国小麦微核心种质低分子量麦谷蛋白Glu-A3位点等位基因的PCR检测[J].分子植物育种,2006,4(4):477-482.
[22] 吴芳,董惠,韩兆雪,等.中国小麦品种高分子量谷蛋白亚基和低分子量谷蛋白亚基组成分析[J].麦类作物学报,2006,26(3):82-86.
[23] 梁强,王晓龙,张晓科,等.陕西小麦Glu-A3和Glu-B3位点等位变异的检测和分析[J].麦类作物学报,2011,31(5):859-864.
[24] 聂迎彬,穆培源,桑伟,等.新疆小麦品种Glu-A3和Glu-B3位点等位变异的分布[J].麦类作物学报,2011,31(5):853-858.
[25] 刘丽,阎俊,张艳,等.冬播麦区Glu-1和Glu-3位点变异及1B/1R易位与小麦加工品质性状的关系[J].中国农业科学,2005,38(10):1944-1950.
[26] 王晓军,冯国华,刘东涛,等.黄淮麦区部分小麦品种(系)1BL/1RS易位的分子检测[J].麦类作物学报,2008,28(3):381-386.
[27] 陈东升,刘丽,董建力,等.HMW-GS和LMW-GS组成及1BL/1RS易位对春小麦品质性状的影响[J].作物学报,2005,31(4):414-419.
[28] 刘丽,于亚雄,胡银星,等.我国秋播麦区小麦Glu-1和Glu-3位点等位变异分析[J].西南农业学报,2004,17(z1):30-34.

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黄淮麦区南片小麦Glu-3位点等位变异分析

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黄淮麦区南片小麦Glu-3位点等位变异分析

Allelic Variation of Glu-3 in Wheat from South Yellow and Huai Valley Facultative Wheat Region in China

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