摘要： 为了利用基因工程手段改变谷子中氨基酸组成和含量,进而改善谷子营养品质,以谷子栽培品种豫谷1号幼嫩、无病斑的叶片基因组DNA为模板,根据已报道的玉米天冬氨酸转氨酶基因序列,设计1对特异性引物扩增克隆谷子天冬氨酸转氨酶基因部分序列。结果表明,PCR扩增获得约750 bp的目标片段,对该片段进行克隆测序得到一条742 bp的片段,经公共数据库同源搜索发现该序列含有天冬氨酸转氨酶超基因家族特有的保守域,证明所克隆片段为天冬氨酸转氨酶基因。对该基因进行系统进化树分析发现,部分单子叶植物和双子叶植物分别聚类,但是谷子与玉米、甘蔗3个C4作物并没有聚为一类。为进一步揭示谷子天冬氨酸转氨酶的功能奠定了基础。

关键词: 谷子, 天冬氨酸转氨酶, 克隆

Abstract: The genomic DNA extracted from young,no-lesion leaves of foxtail millet cultivar Yugu 1 was as template,and a pair of specific primers was designed according to the reported corn aspartate aminotransferase gene sequence to clone aspartate transaminase partial gene sequence of foxtail millet,so as to provide foundation for altering composition and content of amino acids by genetic engineering,further altering nutritional quality of foxtail millet.The results showed that:PCR amplification obtained a 750 bp target fragment.After cloning and sequencing,a 742 bp fragment was obtained.After Blast search through public database,a specific conserved domain belonging to aspartate aminotransferase supergene family was found in cloned sequence,which proved that the cloned fragment was aspartate aminotransferase gene.Then a phylogenetic tree based on the gene sequences was constructed,which indicated that part of monocots and dicots could cluster respectively,but foxtail millet,maize and sugar cane,three C4 plants,could not cluster together.This study provided foundation for further revealing the function of aspartate aminotransferase gene of foxtail millet.

Key words: Foxtail millet, Aspartate aminotransferase, Cloning

中图分类号: 

• S515

• Q78