大豆<em>gma-miR160o</em>启动子的克隆及植物表达载体构建

doi:10.7668/hbnxb.2015.01.004

华北农学报 ›› 2015, Vol. 30 ›› Issue (1): 19-23. doi: 10.7668/hbnxb.2015.01.004

所属专题： 油料作物；

大豆gma-miR160o启动子的克隆及植物表达载体构建

倪志勇, 于月华, 刘超, 任燕萍, 陈全家, 曲延英

新疆农业大学农学院, 新疆农业大学农业生物技术重点实验室, 新疆乌鲁木齐 830052

收稿日期:2014-10-03 出版日期:2015-02-28
通讯作者: 于月华(1981-),女,新疆精河人,讲师,在读博士,主要从事作物抗逆分子育种研究。
作者简介:倪志勇(1981-),男,黑龙江苇河人,助理研究员,博士,主要从事植物逆境分子生物学研究。
基金资助:
新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2014I015);新疆维吾尔自治区自然科学基金资助项目(2014211A025;2013211B19);国家自然基金项目(31360264);新疆农业大学草业科学国家级重点学科项目

Isolation of gma-miR160o Promoter from Glycine max and Construction of Its Plant Expression Vectors

NI Zhi-yong, YU Yue-hua, LIU Chao, REN Yan-ping, CHEN Quan-jia, QU Yan-ying

College of Agronomy, Xinjiang Agricultural University, Key Laboratory of Agricultural Biological Technology, Xinjiang Agricultural University, Urumqi 830052, China

Received:2014-10-03 Published:2015-02-28

摘要/Abstract

摘要： 为了研究miRNA表达的调控机制,以大豆Williams 82基因组DNA为材料,根据预测的 gma-miR160o 启动子序列设计引物,采用PCR方法克隆 gma-miR160o 上游一段长度为1 910 bp的启动子序列。采用PlantCARE和PLACE启动子在线预测工具分析表明, gma-miR160o 启动子序列具有TATA-box、CAAT-box基本的顺式作用元件和一些参与非生物胁迫、光和植物激素应答相关的顺式作用元件。将 gma-miR160o 启动子替代pBI121载体中的CaMV35S启动子,构建了与 GUS 融合的启动子表达载体。

关键词: 大豆, gma-miR160o, 启动子, 植物表达载体

Abstract: In order to investigate the regulation mechanism of miRNA expression, in this study, based on the predicted sequence of gma-miR160o promoter region, primers of promoter sequences were designed.A 1 910 bp fragment was cloned from the genomic DNA of the soybean Williams 82 by PCR method.Promoter sequence analyzed by the database of PlantCARE and PLACE showed that the sequence contained basic elements TATA-box, CAAT-box and some cis -acting elements involved in response to abiotic stresses, light and plant hormones.The genomic DNA fragment in gma-miR160o promoter and GUS fusion expression vector were constructed by replacing CaMV35S promoter of pBI121.

Key words: Soybean, gma-miR160o, Promoter, Plant expression vector

中图分类号:

Q78
S565.03

倪志勇, 于月华, 刘超, 任燕萍, 陈全家, 曲延英. 大豆gma-miR160o启动子的克隆及植物表达载体构建[J]. 华北农学报, 2015, 30(1): 19-23. doi: 10.7668/hbnxb.2015.01.004.

NI Zhi-yong, YU Yue-hua, LIU Chao, REN Yan-ping, CHEN Quan-jia, QU Yan-ying. Isolation of gma-miR160o Promoter from Glycine max and Construction of Its Plant Expression Vectors[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 19-23. doi: 10.7668/hbnxb.2015.01.004.

http://www.hbnxb.net/CN/Y2015/V30/I1/19

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大豆gma-miR160o启动子的克隆及植物表达载体构建

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大豆gma-miR160o启动子的克隆及植物表达载体构建

Isolation of gma-miR160o Promoter from Glycine max and Construction of Its Plant Expression Vectors

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