昼夜温差和光照强度对烟草西柏三烯二醇的合成及 <em>CYC-1</em>、<em>CYP71D16</em>基因表达的影响

doi:10.7668/hbnxb.2014.05.026

华北农学报 ›› 2014, Vol. 29 ›› Issue (5): 150-155. doi: 10.7668/hbnxb.2014.05.026

所属专题： 烟草；生物技术；

昼夜温差和光照强度对烟草西柏三烯二醇的合成及 CYC-1、CYP71D16基因表达的影响

王冬¹, 张小全¹, 彭玉富², 张笛³, 李丽华¹, 杨铁钊¹

1. 河南农业大学烟草学院, 河南郑州 450002;
2. 河南中烟工业公司技术中心, 河南郑州 450002;
3. 河南中烟工业公司许昌卷烟厂, 河南许昌 461000

收稿日期:2014-07-13 出版日期:2014-10-28
通讯作者: 杨铁钊(1955-), 男, 河南临颍人, 教授, 博士生导师, 主要从事烟草遗传育种研究。
作者简介:王冬(1985-), 男, 河南封丘人, 在读博士, 主要从事烟草遗传育种与生理研究。
基金资助:
中国烟草总公司科技重点项目(110201002008)

Effects of Temperature Range and Light Intensity on Content of CBT-diols and Expression of CYC-1 and CYP71D16 in Tobacco

WANG Dong¹, ZHANG Xiao-quan¹, PENG Yu-fu², ZHANG Di³, LI Li-hua¹, YANG Tie-zhao¹

1. College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China;
2. Technology Center of China Tobacco Henan Industrial Corporation, Zhengzhou 450002, China;
3. Xuchang Cigarette Factory of China Tobacco Henan Industrial Corporation, Xuchang 461000, China

Received:2014-07-13 Published:2014-10-28

摘要/Abstract

摘要： 为阐明光照和温度对烟草西柏三烯二醇合成的调控机制,以烟草突变品系8306及其原始株12451为材料,采用气相色谱-质谱联用技术(GC-MS)和实时荧光定量PCR技术,研究了不同昼夜温差和不同光照强度条件下烟草西柏三烯二醇的合成差异以及烟草CYC-1和CYP71D16基因的表达差异。结果表明,昼夜温差15 ℃条件下西柏三烯二醇合成量以及CYC-1和CYP71D16基因表达量明显高于昼夜温差0 ℃处理,说明较大的昼夜温差促进CYC-1和CYP71D16基因上调表达,从而使西柏三烯二醇得到充分的积累;光照强度100 000 lx条件下西柏三烯二醇合成量以及CYC-1和CYP71D16基因表达量明显高于光照强度40 000 lx处理,说明光照不足抑制了CYC-1和CYP71D16的表达,较高的光照强度有利于西柏三烯二醇的合成与积累。

关键词: 西柏三烯二醇, 基因表达, 昼夜温差, 光照强度

Abstract: Using 8306 and 12451 tobacco cultivars as the materials,the effects of temperature range between day and night and light intensity on the content of CBT-diols and the expression of CYC-1 and CYP71D16 were studied.When the temperature range was 15 ℃,the content of CBT-diols and the expression levels of CYC-1 and CYP71D16 were significant higher than those in the temperature range of 0 ℃.This suggests that larger temperature range could promote the up-regulated expression of CYC-1 and CYP71D16,so the CBT-diols accumulated more sufficiently.In the condition of 100 000 lx light intensity,the content of CBT-diols and the expression levels of CYC-1 and CYP71D16 were significantly higher than those in the condition of 40 000 lx light intensity.This suggests that shading inhibited the expression of CYC-1 and CYP71D16 and larger light intensity promoted the synthesis of CBT-diols.

Key words: Cembratrien-diol, Gene expression, Temperature range between day and night, Light intensity

中图分类号:

Q78
S572.03

王冬, 张小全, 彭玉富, 张笛, 李丽华, 杨铁钊. 昼夜温差和光照强度对烟草西柏三烯二醇的合成及 CYC-1、CYP71D16基因表达的影响[J]. 华北农学报, 2014, 29(5): 150-155. doi: 10.7668/hbnxb.2014.05.026.

WANG Dong, ZHANG Xiao-quan, PENG Yu-fu, ZHANG Di, LI Li-hua, YANG Tie-zhao. Effects of Temperature Range and Light Intensity on Content of CBT-diols and Expression of CYC-1 and CYP71D16 in Tobacco[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(5): 150-155. doi: 10.7668/hbnxb.2014.05.026.

http://www.hbnxb.net/CN/Y2014/V29/I5/150

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昼夜温差和光照强度对烟草西柏三烯二醇的合成及 CYC-1、CYP71D16基因表达的影响

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昼夜温差和光照强度对烟草西柏三烯二醇的合成及 CYC-1、CYP71D16基因表达的影响

Effects of Temperature Range and Light Intensity on Content of CBT-diols and Expression of CYC-1 and CYP71D16 in Tobacco

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