摘要： 旨在研究不同物种泌乳奶牛牛乳中活性物质免疫球蛋白和乳铁蛋白绝对含量的特性。采用双抗夹心ELISA法对牦牛乳、水牛乳和荷斯坦奶牛乳乳样中主要免疫球蛋白(IgA、IgG、IgM)和乳铁蛋白(Lf)含量进行了定量测定并做出了相关性分析。结果显示,牦牛乳、水牛乳和荷斯坦奶牛乳乳样中免疫球蛋白和乳铁蛋白活性质量浓度与吸光值存在良好的线性关系,各标准曲线方程的R均大于0.99。不同物种牛乳中IgA、IgG、IgM和Lf的绝对含量差异显著(P<0.05)。同一品种牛乳中免疫球蛋白IgA、IgG、IgM和Lf的绝对含量显示出不同的个体性差异。除荷斯坦奶牛乳乳样中IgA与IgM呈现较低程度的负相关外,牛乳中IgA、IgG、IgM和Lf在同一物种内均呈现出不同程度的正相关趋势。结果表明,不同物种牛乳中免疫球蛋白和乳铁蛋白的绝对含量受遗传因素的影响较大且其含量各具特点,根据同一品种牛乳中IgA、IgG、IgM和Lf含量的相关性可对其含量进行相互预测。

关键词: 物种, 牛奶, 免疫球蛋白, 乳铁蛋白, 酶联免疫, 绝对含量, 相关性

Abstract: The objective of the current study was to characterize the concentrations of immunoglobulins(IgA,IgG,IgM) and lactoferrin in different bovine milk. Sandwich enzyme}inked immunosorbent assay (ELISA) was used to quantify the immunoglobulins and lactoferrin(Lf) contents in Yak milk,Buffalo milk and Holstein cow milk samAles,and correlation analysis was completed. The results showed that immunoglobulins and lactoferrin in Yak milk,Buffalo milk and Holstein cow milk samples had a good linear relationsship between concentration and absorbance values,and the standard curve equation R was greater than 0.99. There were significant differences of IgA,IgG IgM,and Lf in different milk ( P<0.05).The contents of IgA,IgG,IgM and Lf of species showed remarkable individual differences. IgA and IgM in Holstein cow milk showed a negative correlation,while the others species had a positive correlation. The results indicated that the content of immunoglobulins and lactoferrin in the milk were influenced by genetic factors and had each characteristic. According to the correlation of immunoglobulins and lactoferrin, forecasting the content of immunoglobulins and lactoferrin within species may be possible.

Key words: Species, Bovine milk, Immunoglobulin, Lactoferrin, ELISA, Concentration, Correlation

中图分类号: 

• S823