摘要： SIRT1基因对细胞的生存、衰老、凋亡等生理活动起着十分重要的调节作用。为了建立荧光定量PCR技术检测猪SIRT1基因表达量,根据GenBank中猪SIRT1 mRNA序列设计引物,建立基于SYBR Green I染料技术的荧光定量PCR检测方法。结果表明,所建立的荧光定量PCR检测方法具有快速、敏感和特异性强等特点,为猪SIRT1基因定量分析提供了技术平台。

关键词: 猪, SIRT1, 荧光定量PCR

Abstract: SIRT1 gene plays an important regulatory role on cell survival,senescence,apoptosis.This study was to establish a Fluorescent quantitative PCR assay for porcine SIRT1 gene expression.According to the mRNA sequence of SIRT1,primers of porcine SIRT1 gene were designed,and a power SYBR Green Fluorescent quantitative PCR method based on SYBR Green I dye was developed.The results showed that the established Fluorescent quantitative PCR detection method was rapid,sensitive and specific,and so on.The result provided a useful methodological basis for investigating SIRT1 gene.

Key words: Pig SIRT1 gene, Fluorescent quantitative, PCR

中图分类号: 

• Q78