摘要： 为了深入探讨油茶杂交子代的遗传多样性,为油茶杂交育种选育提供指导和理论支持。以4个控制授粉家系的子代共52个个体为研究对象,从105对SRAP引物中筛选出9对条带丰富、多态性高、重复性好的引物,采用优化的SRAP-PCR反应体系对供试材料进行了遗传多样性研究。采用POPGENE3.2和NTSYS2.10软件对扩增结果进行分析,数据显示:共检测到191个位点,扩增片段大小分布在100～700 bp,其中多态性位点191个,平均每对引物21.2个,多态性比率为100%;Nei基因多样性指数(H)为0.333 7～0.366 4,Shannon信息指数(I)为0.503 8～0.545 0。分析结果表明,遗传多样性主要存在于家系内,家系间遗传多样性只占总遗传变异的4.78%,4个控制授粉家系内各子代个体间都存在着丰富的遗传多样性。据此推测,在通过杂交育种技术培育油茶新品种工作中,应以杂交后代个体选择为主,优良家系选择对油茶产量提升的潜力较小。
关键词:
油茶,
家系,
SRAP,
遗传多样性
Abstract: Camellia oleifera is important chinese native woody tree species,and well-bred breeding is primary to selection breeding.In order to further explore the genetic diversity of Camellia oleifera for selecting high-quality seed,in this study for a total of 52 varieties with 4 families,SRAP-PCR of Camellia oleifera was applicated in the basis of optimization system.9 pairs of SRAP primers had been selected from 105 pairs of SRAP primers.POPGENE3.2 and NTSYS2.10 were used to detect the genetic diversity among 52 Camellia oleifera cultivars,and total 191 sites were obtained,The size of the sites being between 100 bp and 700 bp,the average bands per SRAP primer pair being 21.2,and the ratio of polymorphism was 100.00%.Nei index number from 0.333 7 to 0.336 4 and Shannon index number was from 0.503 8 to 0.545 0.Genetic diversity index information indicated that plentiful genetic diversity was existed in this 4 families.The study is expected to provide some technical foundations for the study of the genetic diversity of C.oleifera resources.
Key words:
Camellia oleifera,
Family SRAP,
Genetic diversity
中图分类号:
范小宁, 林萍, 张盛周. 油茶控制授粉子代遗传多样性的SRAP分析[J]. 华北农学报, 2011, 26(S1): 11-17. doi: 10.7668/hbnxb.2011.S1.003.
FAN Xiao-ning, LIN Ping, ZHANG Sheng-zhou. Analysis of Genetic Diversity in Camellia oleifera Germplasms Family[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 11-17. doi: 10.7668/hbnxb.2011.S1.003.