夜间低温对不同基因型番茄叶绿素荧光参数的影响

doi:10.7668/hbnxb.2011.04.040

摘要/Abstract

摘要： 为了明确不同基因型番茄叶片光系统Ⅱ(PSⅡ)活性对夜间低温逆境的响应。以耐低温野生番茄(Lycoper-sicon hirstum)LA1777和低温敏感型番茄(Lycopersicon esculentum Mill.)Moneymaker为试材,研究短期夜间15℃(对照)、9℃和6℃对不同基因型番茄叶片的叶绿素含量和叶绿素荧光参数的影响。结果表明:夜间低温下两种类型番茄幼苗叶片的叶绿素a、b含量及叶绿素a/b值均随着温度降低而呈下降趋势,夜间低温处理1 d后,Moneymaker的叶绿素a和b含量均显著低于对照。夜间低温增加了番茄叶片的Fo(初始荧光)和NPQ(非光化学猝灭系数),显著降低了Fv/Fm(原初光能效率)、ΦPSⅡ(实际光化学量子效率)、ETR(电子传递速率)和qP(光化学猝灭系数)。其中,除NPQ在两番茄叶片的变化趋势相似外,Moneymaker叶片的变化幅度均大于LA1777。这表明夜间低温显著影响了低温敏感番茄叶片PSⅡ反应中心和电子传递,显著降低了PSⅡ的活性,而对耐低温野生番茄PSⅡ活性影响较小。

关键词: 番茄, 夜间低温, 叶绿素含量, 叶绿素荧光参数

Abstract: In order to reveal the effects of low night temperature on chlorophyll content and PS Ⅱ activity in different genotypes tomatoes leaves.The changes of chlorophyll content and chlorophyll fluorescence parameters in chilling-tolerant wild tomato LA1777 and chilling-sensitive tomato Moneymaker under short-time 15℃(control),9℃ and 6℃ night temperature were studied.The results showed that the chlorophyll a,b content and chlorophyll a/b in two genotypes tomato leaves were significantly decreased under low night temperature stress,and the chlorophyll a and b content in Moneymaker significantly decreased than control after 1 d low night temperature treated.The Fo and NPQ increased in two tomatoes leaves under low night temperature,however,Fv/Fm,ΦPS Ⅱ,ETR and qP were decreased significantly after low night temperature treated.Only NPQ in two tomatoes had the same trend,other fluorescence parameters in Moneymaker leaves had greater changed scale than LA1777.The results showed that PS Ⅱ activity and electron transfer in cold-sensitive tomato leaves were influenced under low night temperature,and PS Ⅱ activity decreased significantly,but its decline scale was lower in cold-tolerant tomato.

Key words: Tomato, Low night temperature, Chlorophyll content, Chlorophyll fluorescence parameters

中图分类号:

S641.2

齐红岩, 华利静, 赵乐, 汤羽凡. 夜间低温对不同基因型番茄叶绿素荧光参数的影响[J]. 华北农学报, 2011, 26(4): 222-227. doi: 10.7668/hbnxb.2011.04.040.

QI Hong-yan, HUA Li-jing, ZHAO Le, TANG Yu-fan. Effect of Low Night Temperature on Chlorophyll Fluorescence Parameters in Different Genotypes Tomato Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 222-227. doi: 10.7668/hbnxb.2011.04.040.

http://www.hbnxb.net/CN/Y2011/V26/I4/222

参考文献

[1] AdamsSR,Cockshull K E,CaveC B J. Effect of temperature on the growth and development of tomato fruits[J].Annals of Botany,2001,(88):869-877.
[2] 张子山,张立涛,高辉远. 不同光强与低温交叉胁迫下黄瓜PSⅡ与PSⅡ的光抑制研究[J].中国农业科学,2009,(12):4288-4293.doi:10.3864/j.issn.0578-1752.2009.12.021.
[3] Venema J H,Posthumus F,Vries M de. Differential response of domestic and wild Lycopersicon species to chilling under low light:growth,carbohydrate content,photosynthesis and the xanthophyll cycle[J].Physiologia Plantarum,1999,(1):81-88.doi:10.1034/j.1399-3054.1999.105113.x.
[4] 梁芳,郑成淑,孙宪芝. 低温弱光胁迫及恢复对切花菊光合作用和叶绿素荧光参数的影响[J].应用生态学报,2010,(01):29-35.
[5] 张志刚,尚庆茂. 低温、弱光及盐胁迫下辣椒叶片的光合特性[J].中国农业科学,2010,(01):123-131.doi:10.3864/j.issn.0578-1752.2010.01.015.
[6] 李天来,刘玉凤,宋礼毓. 夜间亚低温处理及其恢复对番茄叶片光抑制的影响[J].园艺学报,2008,(07):1003-1010.doi:10.3321/j.issn:0513-353X.2008.07.010.
[7] 王国莉,郭振飞. 低温对水稻不同耐冷品种幼苗光合速率和叶绿素荧光参数的影响[J].中国水稻科学,2005,(04):381-383.doi:10.3321/j.issn:1001-7216.2005.04.018.
[8] 胡春梅,侯喜林,王旻. 低温胁迫对不结球白菜光合及叶绿素荧光特性的影响[J].西北植物学报,2008,(12):2478-2484.doi:10.3321/j.issn:1000-4025.2008.12.019.
[9] 李合生. 植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.134-137.
[10] 白青华,郭晓冬,王萍. 低温对辣椒幼苗叶片氮及叶绿素含量的影响[J].甘肃农业大学学报,2009,(06):48-51.doi:10.3969/j.issn.1003-4315.2009.06.010.
[11] 胡文海,喻景权. 低温弱光对番茄叶片光合作用和叶绿素荧光参数的影响[J].园艺学报,2001,(01):41-46.doi:10.3321/j.issn:0513-353X.2001.01.008.
[12] Glannakoula A,Ilias I,Papastergiou. The effects of development at chilling temperatures on the function of the photosynthetic apparatus under high and low irradiance in leaves of lettuce (Lactuca sativa L.)[A].Ecosystems and Development,Venice,Italy,2006.396-402.
[13] Garstka M,Venema J H,Rumak I. Contrasting effect of dark-chilling on chloroplast structure and arrangement of chlorophyll-protein complexes in pea and tomato:plants with a different susceptibility to nonfreezing temperature[J].Plants,2007,(05):1165-1181.
[14] 黄伟,任华中,张福墁. 低温弱光对番茄苗期生长的光合作用的影响[J].中国蔬菜,2002,(04):15-17.doi:10.3969/j.issn.1000-6346.2002.04.006.
[15] Bertamini M,Muthuchelian K,Rubinigg M. Low-night temperature increased the photoinhibition of photosynthesis in grapevine (Vitis vinifera L.cv.Riesling) leaves[J].Environmental and Experimental Botany,2006.25-31.doi:10.1016/j.envexpbot.2005.04.002.
[16] Venema H J,Villerius L,Hasselt van R P. Effect of acclimation to suboptimal temperature on chilling-induced photodamage:comparison between a domestic and a high-altitude wild Lycopersicon species[J].Plant Science,2000.153-163.doi:10.1016/S0168-9452(99)00228-9.
[17] 周建,杨立峰,郝峰鸽. 低温胁迫对广玉兰幼苗光合及叶绿素荧光特性的影响[J].西北植物学报,2009,(01):136-142.doi:10.3321/j.issn:1000-4025.2009.01.021.
[18] 刘栓桃,董艳敏,卢亚楠. 低温弱光对两个西葫芦品种幼苗光合速率及叶绿素荧光参数的影响[J].华北农学报,2009,(02):139-143.
[19] 周蕴薇,刘艳萍,戴思兰. 用叶绿素荧光分析技术鉴定植物抗寒性的剖析[J].植物生理学通讯,2006,(05):945-950.
[20] 王晓楠,付连双,李卓夫. 低温驯化及封冻阶段不同冬小麦品种叶绿素荧光参数的比较[J].麦类作物学报,2009,(01):83-88.
[21] 颉建明,郁继华,颉敏华. 耐性不同辣椒幼苗光合和PSⅡ光化学效率对低温弱光的响应[J].中国农学通报,2009,(07):193-197.
[22] 许大全. 光合作用测定及研究中一些值得注意的问题[J].植物生理学通讯,2006,(06):1163-1167.
[23] Krause G H,Weise E. Chlorophyll fluorescence and photosynthesis[J].The Basics Annu Rev Plant Physiol Plant Mol Biol,1991.313-349.
[24] 刘慧英,朱祝军,史庆华. 低温胁迫下嫁接对西瓜光合特性及叶绿素荧光参数影响的研究[J].石河子大学学报（自然科学版）,2007,(02):163-167.doi:10.3969/j.issn.1007-7383.2007.02.008.
[25] 王贺,刘国成,吕德国. 低温对寒富苹果及其亲本叶绿素荧光特性的影响[J].华北农学报,2008,(02):38-40.
[26] 陈青君,张福墁,王永健. 临界低温弱光对黄瓜光合特性及其酶变化的影响[J].华北农学报,2003,(04):31-34.doi:10.3321/j.issn:1000-7091.2003.04.009.

选择文件类型/文献管理软件名称

选择包含的内容