摘要： 探讨禁食状态下孤啡肽及其受体mRNA在脂肪组织中表达的变化.将18只雄性昆明小鼠随机分为正常组、禁食组、再喂食组,采集其脂肪组织,用RT-PCR方法检测孤啡肽及其受体mRNA水平的变化.结果表明,孤啡肽及其受体基因均在小鼠脂肪组织中有表达,与正常组相比,禁食组孤啡肽及其受体的mRNA表达水平明显上调(P＜0.05),而再喂食组表达差异不显著.说明在禁食情况下,孤啡肽及其受体基因对脂肪组织起到一定的调节作用.
关键词:
孤啡肽,
受体,
RT-PCR,
禁食,
脂肪组织
Abstract: To observe effects of fasting on Nociceptin/Orphanin FQ(N/OFQ)and opioid receptor-like 1 mRNA (ORLl)levels of adipose tissue.18 mice were randomly divided into normal group,fasting group and re-feeding group.Adipose tissue was isolatd and analyzed by reverse transcription polymerdse chain reaction(RT-PCR).N/OFQ and ORLl gene were all expressed in adipose tissue.Compared with normal group,the mRNA levels of N/OFQ and ORLl in fasting group increased significantly(P<0.05),but the mRNA levels in re-feeding group has no significant difference.N/OFQ and ORLl may play a role in the regulation of metabolism in adipose tissue.
Key words:
NOFQ,
Receptor,
RT-PCR,
Fasting,
Adipose tissue
中图分类号:
陈福伟, 李文林, 韩立强, 李宏基, 郭豫杰, 王伟杰, 鲁维飞, 谢佳喜, 闫威, 杨国宇. 禁食对小鼠孤啡肽及其受体基因在脂肪组织中表达的影响[J]. 华北农学报, 2011, 26(2): 81-84. doi: 10.7668/hbnxb.2011.02.018.
CHEN Fu-wei, LI Wen-lin, HAN Li-qiang, LI Hong-ji, GUO Yu-jie, WANG Wei-jie, LU Wei-fei, XIE Jia-xi, YAN Wei, YANG Guo-yu. Effects of Fasting on Nociceptin⁄Orphanin FQ and Opioid Receptor-like 1 mRNA Levels of Adipose Tissue in Mice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(2): 81-84. doi: 10.7668/hbnxb.2011.02.018.