不同品种猪背最长肌eIF4E、PPARGC-1、VEGFA和C-Jun mRNA丰度比较研究

doi:10.7668/hbnxb.2010.S1.058

摘要/Abstract

摘要： 为了研究mTOR通路及其相关联途径上与蛋白质合成有关的eIF4E、与能量代谢有关的PPARGC-1、与血管形成有关的VEGFA、与细胞增殖有关的C-Jun4个基因在不同品种猪背最长肌中的表达丰度,选用上市体重的长白猪、地方猪种蓝塘猪和大花白猪各8头作为动物模型,以管家基因GAPDH作为内标,采用Real-time RT-PCR方法,比较这些品种猪的背最长肌中目的基因mRNA表达丰度。结果表明:大花白猪背肌eIF4E mRNA的表达量显著高于蓝塘猪和长白猪(P＜0.05),其在蓝塘猪背肌的表达量高于长白猪但差异不显著(P>0.05);蓝塘猪背肌PPARGC-1mRNA的表达水平显著高于大花白猪(P＜0.05),与长白猪相比差异不显著(P>0.05),长白猪与大花白猪相比无显著差异(P>0.05);VEGFA和C-Jun mRNA在蓝塘、长白、大花白猪背肌中的表达没有差异(P>0.05)。以上结果提示,eIF4E和PPARGC1基因的差异表达可能与不同猪品种间肌肉性状和肉质差异密切相关。

关键词: 猪, 背最长肌, eIF4E, PPARGC-1, VEGFA, C-Jun

Abstract: This study was conducted to compare the mRNA abundant of eIF4E,PPARGC-1,VEGFA and C-Jun gene of longissimus dorsi muscle in different domestic pigs. Those genes,respectively related to protein synthesis,energy metabolism,vascularization and cell proliferation,were selected from mTOR pathway and its related pathways,then real-time RT-PCR was applied to compare the interspecific difference of their mRNA of longissimus dorsi muscle in Lantang,Landrace and Large White-and-Black at the marketing age. It was found that: Large White-and-Black showed significantly highest eIF4E mRNA expression levels of longissimus dorsi muscle,compared with other breeds. The eIF4E mRNA abundant of longissimus dorsi muscle in Lantang was higher than that in Landrace,but the difference was not statistically significant(P > 0. 05) ; the expression level of PPARGC-1 mRNA of longissimus dorsi muscle in Lantang was significantly higher than that in Large White-and-Black(P ＜0. 05), which was also higher than that in Landrace insignificantly(P >0. 05), and the expression level in Landrace was higher than that in Large Whiteand-Black(P >0. 05) ; the expression pattern of VEGFA and C-Jun mRNA were seemed to be not significantly different among these three breeds(P > 0. 05). The comparative results indicated that the differential expression of eIF4E and PPARGC-1 may lead to sarcous trait differences among different domestic pigs.

Key words: Longissimus dorsi muscle, eIF4E, PPARGC-1, VEGFA, C-Jun

中图分类号:

S828

杨舟, 王修启. 不同品种猪背最长肌eIF4E、PPARGC-1、VEGFA和C-Jun mRNA丰度比较研究[J]. 华北农学报, 2010, 25(S1): 258-262. doi: 10.7668/hbnxb.2010.S1.058.

YANG Zhou, WANG Xiu-qi. Comparison of eIF4E,PPARGC-1,VEGFA and C-Jun Gene mRNA Abundance of Longissimus Dorsi Muscle in Different Domestic Pigs(Sus scrofa)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S1): 258-262. doi: 10.7668/hbnxb.2010.S1.058.

http://www.hbnxb.net/CN/Y2010/V25/IS1/258

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